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Monkey Tumor necrosis factor α,TNF-α ELISA KIT

Protocol
Code CSB-E10039Mo
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Alternative Names
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Abbreviation
TNF
Uniprot No.
P48094
Species
Monkey
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.69 pg/mL-300 pg/mL
Sensitivity
2.345 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of monkey TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:1

Average %

96

Range %

89-101

1:2

Average %

93

Range %

89-99

1:4

Average %

88

Range %

82-96

1:8

Average %

92

Range %

85-95

Recovery

The recovery of monkey TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

91

83-95

EDTA plasma (n=4)

97

91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

300

2.574

2.637

2.606

2.452

150

2.221

2.223

2.222

2.069

75

1.501

1.528

1.515

1.361

37.5

0.923

0.955

0.939

0.786

18.75

0.512

0.519

0.515

0.362

9.38

0.325

0.322

0.324

0.170

4.69

0.244

0.240

0.242

0.089

0

0.150

0.157

0.154

　

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Monkey TNF ELISA Kit was designed for the quantitative measurement of Monkey TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Related Products

TNF Proteins

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 4 reviews

Submit a Review here

Sample type: Cell culture supernatant

Sample species: Monkey

Sample dilution: 1:3,1:5 1:10

Review: CSB-E10039Mo was used to detect the culture supernatant of cynomolgus monkey cells. The OD value of standard curve ranged from 3.1274 to 0.2045, and the OD value of sample ranged from 1.112 to 0.109.

By Anonymous

Sample type: Serum

Sample species: Monkey

Sample dilution: No dilution

Review: I used the monkey Tumor Necrosis Factor α (TNF-α) enzyme-linked immunosorbent assay kit with product number CSB-E10039Mo to detect monkey serum. The standard curve fitting was good, and the accuracy of the back-calculated concentration for each point on the standard curve was less than 10%.

By Anonymous

Sample type: Serum

Sample species: Monkey

Sample dilution: No dilution

Review: I used the monkey Tumor Necrosis Factor α (TNF-α) enzyme-linked immunosorbent assay kit with product number CSB-E10039Mo to detect monkey serum. The standard curve fitting was good, and the accuracy of the back-calculated concentration at each point of the standard curve was less than 10%.

By Anonymous

Sample type: Serum

Sample species: Monkey

Sample dilution: No dilution

Review: I used the monkey TNFa ELISA kit to detect monkey serum, and the results were good. The standard curve was fitted with a four-parameter model, the coefficient of determination was greater than 0.99, the accuracy of each point on the standard curve was between 80-120%, and the difference between duplicate wells was less than 15%.

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. These interactions result in the induction of the TNF signaling pathway, activation of apoptosis, and DNA-damage stress response. PMID: 24743303
  2. Findings showed that both mucosal compartments harbor similar percentages of memory CD4(+) T cells and displayed comparable cytokine TNF-alpha responses to mitogenic stimulations prior to infection. PMID: 24610016
  3. Primary role for IL-1beta and TNF-alpha in the triggering of preterm labor associated with inflammation or infection. PMID: 17132473
Subcellular Location
Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families
Tumor necrosis factor family
Database Links
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Reward For Reviews
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MAPK signaling pathway mTOR signaling pathway TGF-beta signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Antigen processing and presentation Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications Inflammatory Pain
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