Recombinant Macaca mulatta Tumor necrosis factor protein (TNF), partial, (Active)

TNF-α is a master regulator of NF-κB and MAPK signaling cascades, making precise functional characterization in non-human primate models essential for translational oncology and inflammation research. This tag-free recombinant rhesus macaque TNF protein (aa 77–233) demonstrates exceptional signaling potency, with an ED50 below 0.05 ng/ml in the murine L929 cytotoxicity assay—corresponding to a specific activity exceeding 2.0 × 10⁷ IU/mg—providing a reliable basis for NF-κB pathway activation studies, apoptosis assays, and dose-response benchmarking as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that confound immune cell activation and differentiation experiments, while purity greater than 95% by SDS-PAGE supports use in antibody development workflows including immunization, ELISA coating, and positive control applications. The mature sequence fidelity of this E. coli-expressed protein aligns with standards expected in preclinical tumor biology models requiring species-matched cytokine stimulation.