Mouse Leptin,LEP ELISA kit

Instructions
Code CSB-E04650m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names Lep ELISA Kit; Ob ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation LEP
Uniprot No. P41160
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision   
Linearity    
Recovery   
Typical Data   
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

Please could you tell us if you have a Mouse Leptin ELISA Kit that can
be used on only 5 ul of serum?

A:
Thanks for your inquiry.
5ul sample is really too little and at least 1:20 dilution is needed if you dilute the sample.
However, for this kit CSB-E04650m, usually there is no need to dilute serum and plasma sample.
So we hope you could try to collect more samples and make the experiment according our manual.

Target Data

Function Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways
Gene References into Functions
  1. In C57bl/6 model, glycaemic index of maternal dietary carbohydrates differentially alters Fto and Lep expression in offspring. PMID: 30241328
  2. A potent, selective, and orally bioavailable inhibitor of the protein-tyrosine phosphatase PTP1B improves insulin and leptin signaling in animal models PMID: 29217773
  3. Catalase overexpression modulates metabolic parameters in a new 'stress-less' leptin-deficient mouse model. PMID: 28645653
  4. Since upregulation of ANGPTL6 is induced on metabolic stress, we investigated the hepatic expression of ANGPTL6 by leptin, a representative adipokine of obesity. Mice on a high-fat diet showed increased serum leptin levels and hepatic Angptl6 expression. PMID: 29852166
  5. This study shows that prolonged exposure to high-fat diets in adulthood is associated with a gradually increasing DNA methylation level at the Leptin and Pparg2 promoters in a depot-specific manner. PMID: 28256596
  6. the study reports a novel mechanistic pathway of leptin-mediated renal inflammation that is dependent on NOX-2-miR21 axis in ectopic manifestations underlying non-alcoholic fatty liver disease-induced co-morbidities PMID: 29660503
  7. These results demonstrated a novel molecular mechanism by which leptin sustained oligodendrocyte precursor cell proliferation and remyelination in a pathological central nervous system. PMID: 28091609
  8. These data demonstrate that FAAH activity is required for leptin's hypophagic effects PMID: 29967158
  9. These data uncouple the mechanisms conferring qualitative and quantitative expression of the leptin gene and further suggest that factor(s) that bind to LepRE1 quantitatively control leptin expression and might be components of a lipid-sensing system in adipocytes. PMID: 29891714
  10. These results indicate that leptin plays a role in nociception induced by acute inflammation PMID: 29204732
  11. results suggest that STAT transcriptional activity is downregulated by high levels of leptin, leading to reduced cAMP-dependent steroidogenic genes (Star and Cyp11a1) expressions in MA-10 Leydig cells. PMID: 28343310
  12. Data suggest that lnc-leptin is among the most prominent lncRNAs expressed in adipocytes; lnc-leptin expression from an enhancer region upstream of leptin is sensitive to insulin and correlates to leptin expression across diverse pathophysiological conditions; induction of lnc-leptin is essential for adipogenesis; its presence is required for maintenance of leptin expression in adipocytes. (lnc = long non-coding RNA) PMID: 29519872
  13. Data (including data from studies using mutant, transgenic, and knockout mice) suggest that gene targets of leptin/leptin-receptor (Lep/Lepr) signaling in hypothalamic neurons regulate energy metabolism; Lep/Lepr signaling appears to up-regulate expression of Atf3 (activating transcription factor-3) in hypothalamic neurons. PMID: 29535089
  14. Study shows that compared to the other mouse lines, db/db mice with dysfunctional leptin receptors had a significantly longer tail flick latency after saline and buprenorphine. The results provide novel support for the interpretation that acute thermal nociception is associated with altered leptin signaling. PMID: 28893589
  15. The studies established a potential link between leptin and adipocyte insulin responsiveness in an NOS2 dependent manner. PMID: 28739528
  16. leptin regulates the expression of osteocalcin in growth plate chondrocytes via the ERK1/2 signaling pathway, while there is no effect on the phosphorylation of either p38 or AKT PMID: 27564111
  17. Hyperleptinemia exacerbates high fat diet-mediated atrial fibrosis and atrial fibrillation. PMID: 28257569
  18. Taken together, these results suggest that leptin, in concert with other stimuli in the micro milieu, may contribute to the development of psoriasis. PMID: 27488462
  19. Thus, Mc4r in the PVH appears to be required for early-life programming of hypertension arising from either maternal obesity or neonatal hyperleptinemia. Early-life exposure of the PVH to maternal obesity through postnatal elevation of leptin may have long-term consequences for cardiovascular health. PMID: 27791019
  20. t the cellular level, leptin promoted effector T-cell responses and facilitated the presentation of self-antigens to T cells, whereas it inhibited the activity of regulatory CD4 T cells. The understanding of the role of leptin in modulating autoimmune responses in SLE can open possibilities of leptin-targeted therapeutic intervention in the disease. PMID: 27588900
  21. in congenitally leptin deficient animals, leptin is not required for compensatory reduction in energy expenditure accompanying weight loss, but suggest that the hyperphagia of the weight-reduced state is leptin-dependent. PMID: 29261744
  22. Our data provide evidence that the interplay of these two hormones could help improve the understanding of the pathogenesis of atherosclerosis and NAFLD. PMID: 29158088
  23. Data suggest that Htr2c and leptin in hypothalamic nuclei may be involved in the effects of corticosterone on food intake/appetite regulation. (Htr2c = 5-hydroxytryptamine type 2C receptor) PMID: 28186389
  24. Further experiments indicated that miR-122 inhibited leptin-induced liver fibrosis in leptin-deficient mouse model. PMID: 29054053
  25. Endoplasmic reticulum stress-induced CHOP activation in the brain is a mechanistic link in the palmitate-induced negative regulation of leptin and IGF1. PMID: 27555288
  26. neuronal Rap1 critically regulates leptin sensitivity PMID: 27626668
  27. Upregulation of leptin levels in both the vessel wall and the circulation after vessel injury promoted the migration of Sca-1(+) progenitor cells via leptin receptor-dependent signal transducer and activator of transcription 3- Rac1/Cdc42-ERK (extracellular signal-regulated kinase)-FAK pathways, which enhanced neointimal formation. PMID: 28935755
  28. Depletion of CLPABP disturbed the normal subcellular localization of HuR to stress granules, and overexpression of CLPABP induced instability of leptin mRNA by inhibiting HuR function. PMID: 27616329
  29. These results confirm and extend the previous evidence that LEP has a general and important role in the response of mammalian cells to irradiation. PMID: 28593811
  30. leptin is involved in the proliferation and activation of microglia, which in turn enhances the development of neuropathic pain after avulsion of the cervical roots. PMID: 28822786
  31. Suggest that intact endothelial leptin signaling limits neointima formation and that obesity represents a state of endothelial leptin resistance. PMID: 28705795
  32. these studies demonstrate marked differences in the acute insulin-independent effects by which leptin reverses fasting hyperglycemia and ketoacidosis in a rodent model of DKA versus the chronic pleotropic effects by which leptin reverses hyperglycemia in a non-DKA rodent model of T1D. PMID: 28112679
  33. High fat diet attenuates leptin signaling throughout the brain, but some brain regions maintain their ability to sense leptin. Weight loss restores leptin sensing to some degree in most (but not all) brain regions, while other brain regions display hypersensitivity to leptin following weight loss PMID: 28107353
  34. CNS-specific Tak1 deletion prevented ER-stress-induced hypothalamic leptin resistance and hyperphagic obesity under a high-fat diet (HFD). Thus, TAK1 is a crucial regulator of ER stress in vivo, which could be a target for alleviation of ER stress and its associated disease conditions. PMID: 26985063
  35. these data unmasked a role for mitochondrial fission in leptin sensitivity and glucose sensing of POMC neurons. PMID: 28190775
  36. Obesity and leptin deficiency substantially decreased morphine metabolism and clearance. PMID: 27782940
  37. These findings suggest that by impairing the testicular LEP-JAK-STAT pathway, early-stage obesity inhibits the biosynthesis of testosterone and sexual development and reduces male reproductive potential. Long-term moderate and high-volume exercise can effectively reduce body fat and improve obesity-induced abnormalities in testicular leptin signal transduction, whereas only moderate-volume exercise can reverse the negativ PMID: 28100475
  38. genetic and pharmacological ablation of adult NG2-glia (also known as oligodendrocyte precursors), but not microglia, leads to primary leptin resistance and obesity in mice. PMID: 27166944
  39. a novel mechanism of leptin-induced fatty acid oxidation in muscle tissue; namely, this process is dependent on the activation of AMPK to induce the translocation of FAT/CD36 to the plasma membrane, thereby stimulating fatty acid uptake. PMID: 27827305
  40. Results demonstrate that in white adipose tissue, leptin expression and secretion is promoted by Epac1, hence regulating energy balance and glucose homeostasis. PMID: 27381457
  41. may regulate growth hormone expression in somatotropes through the stimulation of POU1F1 PMID: 27571135
  42. This study found that the expression of leptin and its receptor OB-R in mouse models of Sjogren's syndrome are elevated both locally and systemically during Sjogren's syndrome progression. PMID: 27889607
  43. obesity-associated elevation of leptin contributes to the increased susceptibility of asthma via modulation of pro-allergic lymphocyte responses. PMID: 27566543
  44. Leptin stimulation led to TNFalpha production under both in vitro and in vivo conditions, and diurnal fluctuation of leptin concentrations in the cerebrospinal fluid predicted the circadian changes of TNFalpha gene expression in the hypothalamus. PMID: 27226618
  45. geniposide may regulate tau phosphorylation through leptin signaling PMID: 26496899
  46. Activated FXR inhibits leptin signaling and counteracts tumor-promoting activities of cancer-associated fibroblasts in breast malignancy. PMID: 26899873
  47. this study shows that knockdown of leptin in hepatoma cells caused higher Treg activity and lower CD8+ T-cell response in the mouse hepatocellular carcinoma allograft model. PMID: 26639061
  48. Hexim1 selectively modulates leptin-mediated signal transduction pathways in the hypothalamus. PMID: 26859361
  49. The weight-reducing hypophagic effects of leptin are not augmented through its thermogenic effect. PMID: 26876182
  50. ime-specific changes in promoter associated DNA methyltransferases may be associated with the regulation of leptin expression. PMID: 25433105
  51. These data suggest that the heavy infiltration of adipocytes and the presence of leptin in the thymuses of tumor bearers may affect normal thymopoiesis, leading to the impairment of T cell development observed during tumor-induced thymic atrophy. PMID: 26530443
  52. Leptin resistance-induced obesity and diabetes accelerates the development of tau pathology PMID: 26701291
  53. Obese leptin-deficient (ob/ob) mice demonstrate defects in upper airway structural and neuromuscular control PMID: 26472867
  54. recent data showing that diet-induced obesity mice may conserve a cellular and physiological response to endogenous leptin, highlights the need to redefine the concept of "leptin resistance"--{REVIEW] PMID: 26410445
  55. Leptin-deficient mice had lower cancellous bone volume and higher bone marrow adiposity. PMID: 26370912
  56. FABP4 reduced the expression of leptin, CPT-1 and AOX1 in mice adipocytes PMID: 26310911
  57. leptin deficiency results in sustained orexigenic stimulation, leading to increased water diffusion parameters PMID: 25968479
  58. These results suggest the possibility for a direct role for the PDE3B pathway in mediating leptin action in the hypothalamus. PMID: 26297880
  59. leptin activates the cGH gene in the presence of chPit-1alpha via several leptin-activated kinases PMID: 26403688
  60. Data suggest that both adipocyte and somatotrope leptin contribute to the metabolic homeostasis. PMID: 26859333
  61. Tau effects upon feeding behavior control are complex and involve interactions with a vast gene network, preventing hypothalamic leptin resistance. PMID: 26133737
  62. These data suggest that exogenous leptin may promote protection during sepsis, and downregulation of the insulin levels and upregulation of corticosterone may be important mechanisms in the amelioration of LPS-induced ALI. PMID: 24685581
  63. Leptin induces expression of the neuropeptide cocaine- and amphetamine-regulated transcript (CART) in the granulosa cells (GCs) of ovarian follicles both in vitro and in vivo. PMID: 26730935
  64. The adipokine leptin mediates muscle- and liver-derived IGF-1 in aged mice. PMID: 26220769
  65. Leptin upregulated Hedgehog signaling pathway in the process of adipocyte differentiation. PMID: 26648290
  66. These findings provide novel insights into the regulation of leptin production by adipose tissue and open new avenues for examining the influence of variation in leptin levels on adiposity and metabolic health. PMID: 26833098
  67. Circadian disruption induces Leptin resistance independent of other risk factors. PMID: 26166747
  68. Lack of adipocyte leptin regulation and fasting-induced immunosuppression in obese immunodeficient mice strongly suggests that lymphocytes are involved in the modulation of adipocyte leptin expression. PMID: 25670497
  69. Central arachidonic acid administration reduces central leptin sensitivity. PMID: 25986657
  70. Results indicate that leptin is involved in the regulation of dorsal root ganglion development and oxaliplatin-induced neuropathic pain PMID: 26302162
  71. Suggest that unidentified leptin-induced circulating factors that control white fat mass. PMID: 26468261
  72. blocking the leptin-deficient increase in neuropeptide Y (NPY) expression reverses the cortical bone loss in ob/ob mice. PMID: 23044938
  73. These findings demonstrate that hyperleptinemic DIO mice retain leptin suppression of feeding comparable to lean mice PMID: 25980347
  74. Hcrt neuronal activity and Hcrt-mediated stress responses were inhibited by the satiety hormone leptin. PMID: 25695914
  75. Upregulation of miR21 and repression of Grhl3 by leptin mediates sinusoidal endothelial injury in experimental nonalcoholic steatohepatitis. PMID: 25658689
  76. Data show that maternal hyperleptinemia during pregnancy did not predispose offspring to obesity, and in fact, reduced weight gain. PMID: 26196541
  77. leptin signaling is required for adaptive changes in both energy intake and thermal conductance. PMID: 25756181
  78. Study finds that the lipolytic effect of leptin is mediated through the action of sympathetic nerve fibers that innervate the adipose tissue. Using intravital two-photon microscopy, sympathetic nerve fibers establish neuro-adipose junctions, directly "enveloping" adipocytes were observed. PMID: 26406372
  79. These findings indicate that levels of dietary iron play an important role in regulation of appetite and metabolism through CREB-dependent modulation of leptin expression. PMID: 26301810
  80. 1,25-Dihydroxyvitamin D regulates expression of the tryptophan hydroxylase 2 and leptin genes: implication for behavioral influences of vitamin D. PMID: 26071405
  81. leptin of dermal white adipose tissue might regulate hair growth and, therefore, hair cycle progression via leptin receptor on the hair follicle epithelium PMID: 25313970
  82. Data indicate that the regulation of leptin transcriptional expression by n-3 polyunsaturated fatty acids (n-3 PUFAs) is mediated, at least in part, by epigenetic targets. PMID: 24923522
  83. These data suggest that the appetite suppression in WT mice after parabiosis to diabetic mutants is the result of induced hyperleptinemia combined with the stress or other aspect(s) of the parabiosis procedure. PMID: 26150485
  84. Abeta accumulation and apoE4 genotype result in a transient enhancement of leptin signalling that might lead to a leptin resistance state over time. PMID: 25453257
  85. Report leptin-mediated endothelial cell differentiation and angiogenesis in murine embryonic stem cells. PMID: 25250519
  86. CB1 activation reverses leptin-induced ROS formation and hence possibly some of the ROS-mediated effects of the hormone by preventing PPAR-gamma inhibition by leptin, with subsequent increase of catalase activity PMID: 25869131
  87. Leptin inhibit the differentiation of RAW264.7 cells into osteoclasts via inhibiting the expression of PPARgamma PMID: 25652850
  88. Absence of Mecp2 disrupted body weight balance by altering post-translational modifications in leptin-signalling components that regulate Pomc and Agrp expression. PMID: 24996410
  89. These data demonstrate that leptin can modulate DC function and suggest that leptin may dampen T-cell responsiveness in the physiological setting. PMID: 24966213
  90. data suggest that both adipose and placenta-derived leptin system are involved in the regulation of energy metabolism during fetal growth. PMID: 24694605
  91. Leptin analogs can be developed through chemical modification of the native leptin with P85 to overcome leptin resistance at the level of the BBB, thus improving the potential for the treatment of obesity. PMID: 24881856
  92. Suggest that under conditions of sustained increases in hexosamine biosynthetic pathway activity there is an increase in basal STAT3(Y705) phosphorylation that cannot be further increased by leptin. PMID: 25568075
  93. We propose that as yet unidentified humoral factors released from intestinal epithelial cells are involved in regulating circulating leptin and adiponectin levels. PMID: 25656573
  94. Data suggest FoxO1 (forkhead box O1) 140-160 (Gln145, Arg147, Lys148, Arg153, Arg154) is critical for FoxO1-Stat3 (signal transducer/activator of transcription 3) interaction/down-regulation of Lep-induced Pomc (pro-opiomelanocortin) transcription. PMID: 25510553
  95. Our data demonstrate that activation of cardiac fibroblast was inhibited by MSCs in a manner that was leptin-dependent. PMID: 25116394
  96. leptin stimulates synaptogenesis via activation of the MAPK pathways and induction of CREB dependent transcription. PMID: 24877561
  97. Suggest novel role of leptin-NADPH oxidase induction of miR21 as a key regulator of TGF-beta signaling and fibrogenesis in experimental and human NASH. PMID: 25501551
  98. Studies identify a mechanism in which proopiomelanocortin neurons integrate insulin and leptin feedback to drive white adipose tissue browning and maintain energy homeostasis. PMID: 25594176
  99. PLD1 acts as an important regulator of leptin-induced expression of TNF-alpha. PMID: 25047119
  100. Hypothalamic leptin and insulin signals relay information about energy status and are reported to promote thermogenesis, raising the possibility that they interact to direct an appropriate response to nutritional and thermal challenges. PMID: 25125486

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Involvement in disease Defects in Lep are the cause of profound obesity and type II diabetes.
Subcellular Location Secreted
Protein Families Leptin family
Database Links

KEGG: mmu:16846

STRING: 10090.ENSMUSP00000067046

UniGene: Mm.277072

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