Code | CSB-E08854r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 87 | ||||
Range % | 80-95 | |||||
1:2 | Average % | 95 | ||||
Range % | 85-99 | |||||
1:4 | Average % | 95 | ||||
Range % | 86-102 | |||||
1:8 | Average % | 98 | ||||
Range % | 82-104 |
The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 85-100 | ||||
EDTA plasma (n=4) | 95 | 87-102 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Rat BCL2 ELISA Kit was designed for the quantitative measurement of Rat BCL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
Sample type: Serum
Sample species: Rat
Review: Serum Bcl-2 activity in a different group. N ¼ 8, p<0.05 for the group versus control group, &p <0.05 for the group versus DM group, #p <0.05 for the group versus CI group, $p <0.05 for the group versus DMCI group.
By Anonymous
Sample type: Tissue homogenate(intestine)
Sample species: Rat
Review: Bcl-2/Bax ratio content on the intestinal mitochondrial fraction before and after treatment with the specially formulated chamomile extract (100 and 300 mg/kg, twice daily, po) or 1% methylcellulose (vehicle). Results are represented as mean ± s.e.m (n = 10).
By Anonymous
Sample type: Tissue homogenate(colon tissue)
Sample species: Rat
Review: B-cell lymphoma-2 (BCL-2) in AA-induced UC (ulcerative colitis) in rats. Data are expressed as mean ± SD (n = 4). Statistical analysis was performed using ordinary one-way ANOVA.
By Anonymous