Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit

Instructions
Code CSB-E08854r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name B-cell CLL/lymphoma 2
Alternative Names Bcl2 ELISA kit; Bcl-2Apoptosis regulator Bcl-2 ELISA kit
Abbreviation BCL2
Uniprot No. P49950
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-95  
1:2 Average % 95  
Range % 85-99  
1:4 Average % 95  
Range % 86-102  
1:8 Average % 98  
Range % 82-104  
Recovery
The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-100  
EDTA plasma (n=4) 95 87-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.745 2.768 2.757 2.572  
10 2.176 2.165 2.171 1.986  
5 1.485 1.463 1.474 1.289  
2.5 0.896 0.864 0.880 0.695  
1.25 0.569 0.543 0.556 0.371  
0.625 0.425 0.439 0.432 0.247  
0.312 0.296 0.284 0.290 0.105  
0 0.185 0.184 0.185    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Sample type: Tissue homogenates
    Sample species: Rat
    Review: Bcl-2/Bax ratio content on the intestinal mitochondrial fraction before and after treatment with the specially formulated chamomile extract (100 and 300 mg/kg, twice daily, po) or 1% methylcellulose (vehicle). Results are represented as mean ± s.e.m (n = 10)
    PMID: 30632234
  • Sample type: Tissue homogenates
    Sample species: Rat
    Review: B-cell lymphoma-2 (BCL-2) in AAinduced UC in rats. Data are expressed as mean ± SD (n = 4). Statistical analysis was performed using ordinary one-way ANOVA.
    PMID: 30594690
  • Sample type: Blood samples
    Sample species: Rat
    Review: Serum Bcl-2 activity in different group. N ¼ 8, p<0.05 for the group versus control group, &p <0.05 for the group versus DM group, #p <0.05 for the group versus CI group, $p <0.05 for the group versus DMCI group.
    PMID: 31038022

Target Data

Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (By similarity).
Gene References into Functions
  1. The findings suggest that experimental hyperthermia (EH) exposure leads to simultaneous activation of molecular switches of apoptosis (BCL2 and BAD) in cells of the follicular epithelium of the ovaries on days 3 and 4 after EH. PMID: 29658076
  2. miRNA-15b deteriorates cardiomyocyte apoptosis by post-transcriptionally downregulating the expression of Bcl-2 and MAPK3. PMID: 28814571
  3. Melatonin, but not curcumin, significantly increased the Bcl-2 expression of the hippocampal region. There was a significant correlation between SIRT2 and MDA levels. Results suggest that melatonin may increase cell survival in the hippocampus via decreasing oxidative stress and SIRT2 expression and increasing Bcl-2 expression. PMID: 29325994
  4. The expression of Bax and Bcl-2 after ligation of the parotid gland is closely related to the process of the parotid gland atrophy. PMID: 29243771
  5. Data suggest that neuroprotection via ghrelin involves up-regulation of Bcl-2 (B cell lymphoma 2 associated oncogene) and p-62/Sqstm1 (ubiquitin-binding protein p62) plus down-regulation of LC3-II (microtubule-associated protein 1 light chain 3) and Beclin-1. Studies were conducted in adult hippocampal neural stem cells in primary culture. PMID: 29057768
  6. Treatment of ellagic acid resulted in increased neuron viability, cell nuclear integrity, and the ratio of Bcl-2/Bax expression in the primary cultured neuron model. PMID: 28388366
  7. High expression of prosurvival BCL2 proteins and inhibition of apoptosis and autophagy prolongs Sertoli survival upon exposure to stress stimuli. PMID: 28379541
  8. Changes in p53 and Bcl-2 expression are spatially correlated to degenerated hair cells in aging rat cochleae. PMID: 28093932
  9. miR-486 may regulate cardiomyocyte apoptosis via p53-mediated BCL-2 associated mitochondrial apoptotic pathway. PMID: 28486954
  10. These data validated that SRF responded to hypoxia, which subsequently was involved in pulmonary hypertension by abnormally promoting viability of PASMCs via modulating expression of Bcl-2. PMID: 28176371
  11. upon CHIP knockdown, the expression of Bax and caspase-3 mRNAs increased even further, and the expression of Bcl-2 mRNA was further suppressed. The expression of the phosphorylated p65 and p38 proteins (p-p65 and p-p38) was also further enhanced. Thus, CHIP is a potent cardioprotective molecule. PMID: 28257878
  12. These findings indicated for the first time that pretreatment of H9c2 cells with catalpol can be against H2O2-induced apoptosis, and the protective effect of catalpol involves the mitochondrial-dependent caspase pathway and is associated with increased Bcl-2 and decreased Bax expression. PMID: 27166426
  13. Acute ethanol exposure induced autophagy-mediated heart toxicity and injury mainly through the ROS-JNK-Bcl-2 signaling pathway. PMID: 28369910
  14. Bcl2 protein content of skeletal muscle was increased after 8 weeks of treadmill running in aging rats. PMID: 27526155
  15. Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene PMID: 27645924
  16. Data suggest that microRNA miR-98 might promote chondrocyte apoptosis and cartilage degradation by down-regulating Bcl-2 expression in the pathogenesis of osteoarthritis (OA), and that miR-98 can be a potential target for the treatment of OA. PMID: 27590063
  17. Morin-5'-sulfonic acid sodium salt prevents changes in the expression of apoptosis related proteins caspase 3, Bax and Bcl 2 due to mercury induced oxidative stress. PMID: 27919736
  18. Chrysin prevents apoptosis by upregulating the Bcl-2 mRNA expression and downregulating the pro-apoptotic (Bax, Bad) mRNAs in 3-nitropropionic acid induced behavioral despair/mitochondrial dysfunction/striatal apoptosis. PMID: 27690136
  19. Morinda citrifolia augmented the expression of Bcl2 in the skeletal muscle of intra-nigrally rotenone-infused Parkinsonian rats PMID: 26697948
  20. Hypoxia response element Bcl2 inhibition can effectively attenuate hypoxia-induced apoptosis resistance in pulmonary microvascular endothelial cells by downregulating Bcl-2 expression and upregulating caspase-3 and P53 expression. PMID: 26456506
  21. VPA protects myocardial cells from hemorrhagic and hypoxic stress through the Akt/BCL-2 survival pathway, indicating a potential use of HDACIs for acute severe hemorrhage treatment. PMID: 26886004
  22. TP53, BCL-2, and VEGFA genes may participate in the proliferation of esophagus cancer cells in a synergistic manner PMID: 26439224
  23. The spinal cord may be protected from spinal cord ischemia reperfusion injury by inhibition of BAX and activation of BCL2. PMID: 26400280
  24. Intermittent hypobaric hypoxia preconditioning activated the expression of neuroglobin and Bcl-2 in the rat hippocampal CA1 area following ischemia-reperfusion, and prevented hippocampal neuronal apoptosis. PMID: 26400308
  25. Pre-ischemic exercise prevented apoptosis in hippocampal CA3 neurons after cerebral ischemia by attenuating Bax/Bcl-2 protein ratio. PMID: 26012958
  26. MicroRNA34a induces apoptosis in PC12 cells by reducing expression of BCL2 and SIRT1. PMID: 26252661
  27. ISO increased NOXs-derived ROS which activated nuclear translocation of calpain-2, subsequently nuclear calpain-2 degraded CaMK II dB which reduced the ratio of Bcl-2 to Bax and mitochondria apoptosis pathway was triggered in rat cardiomyocytes PMID: 25820554
  28. The present study aimed to study the changes in P53, Bcl-2 and CD68 expression in response to amethopterin-induced lung injury and ameliorating the role of l-carnitine. PMID: 24986327
  29. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels PMID: 26125843
  30. Bcl-2 expression is increased during neuronal differentiation of neural stem cells. PMID: 24986006
  31. Findings indicate that Bcl-2 phosphorylation and thereby Bcl-2/Beclin1 complex disruption play a crucial role in triggering autophagy and reducing mitochondrial damage in RIC rats after cerebral ischemia and require the involvement of the AKT activation. PMID: 25744447
  32. ROS/Bcl-2/Bax and the ROS/microcirculation/ starving pathway control the effect of scutellarin on type II diabetes mellitus-induced testicular damages PMID: 25861655
  33. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1alpha activity. PMID: 26319553
  34. DSW drinking reduced ulcer area as well as apoptotic signaling in acetic acid-induced duodenal ulcers. DSW, which contains selenium, provides intestinal protection against duodenal ulcers through the upregulation of Bcl-2 and thioredoxin reductase PMID: 24984066
  35. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis;tissues with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. PMID: 26003800
  36. Bcl-xL and Bcl-2, in a PI3K-dependent manner, which is upstream of NF-kappaB activation. PMID: 25220584
  37. The level of Bax mRNA and protein increased, while the content of Bcl2 mRNA and protein decreased 30 min after tetanization of the CA1 field of rat hippocampal slices. PMID: 25403402
  38. It has been found that NaBut reduces proliferation rate of ERasBcl (E1A, cHa-ras and bcl-2 ) cells significantly weaker than of ERas transformed cells PMID: 26035971
  39. up-regulated SSTR1 contributed to neuronal apoptosis after intracerebral hemorrhage, which was accompanied with reduced expression of bcl-2. PMID: 25035058
  40. oxymatrine can attenuate the development of UC by regulating the DOR-beta-arrestin1-Bcl-2 signal transduction pathway. PMID: 25480575
  41. the effects of mobile phones on the Bcl-2 gene and p53 proteins in rat brains PMID: 24763879
  42. Overexpression of Bcl-2 in the transplanted NESCs enhanced differentiation. PMID: 23324128
  43. Which might result in the disassociation of Bcl-2 from the Bad/Bcl-2 complex. PMID: 24957688
  44. Studied the effect of acute renal ischemia reperfusion on brain tissue and results showed decreased Bcl-2 expression and upregulated expression of NF-kappaB. PMID: 25063066
  45. Treatment with 6-OHDA reduced PC12 Bcl-2 mRNA levels by about fourfold. CDNF pretreatment attenuated this. PMID: 24633814
  46. Hepatocyte apoptosis could promote NAFLD progression; Fas, FasL, and Caspase-8 mRNA activation were important contributing factors to NAFLD. The upregulation of Bax and Bcl-2 expression might be one important mechanism of the apoptosis in NAFLD. PMID: 24938610
  47. results indicated that after TBI in rats CXCL12 combing CXCR4 receptors could inhibit the caspase-3 pathway by upregulating Bcl-2:Bax ratio, which protect neurons from apoptosis PMID: 23984821
  48. These results suggest the involvement of Bcl-2 and BDNF in processes of adaptation to SH and compensation of its damaging effects. PMID: 25282819
  49. Suggest that miR-21 promotes the development of heart failure with preserved left ventricular ejection fraction by up-regulating the expression of anti-apoptotic gene Bcl-2 and thereby promoting cardiac fibrosis. PMID: 24551276
  50. A critical role of NF-kappaB-mediated miR-30b modulation in Ang II-stimulated cardiomyocytes targeting Bcl-2. PMID: 24178239
  51. Enhanced espression of Bcl2 is required to sustain oxoglutarate carrier-dependent glutathione transport into the mitochondria. PMID: 24606213
  52. Studied curcumin effects on the activities and gene expression in diabetic rats. Curcumin treated rats showed significant increase in gene expression of IGF-1, Bcl2, SOD and GST compare to non diabetic and diabetic untreated rats. PMID: 24364912
  53. we found that Bcl-2 overexpression in the brain enhanced the generation of newborn striatonigral projection neurons PMID: 23225311
  54. The Bax/Bcl-2 apoptotic pathway is not responsible for the increase in apoptosis in the RU486-treated rat uterus during early pregnancy. PMID: 24287037
  55. Prohibitin inhibits apoptosis in granulosa cells through the ERK1/2 pathway and the Bcl2 and Bcl-xL. PMID: 24096434
  56. identification of the 3'-UTR of Bcl-2 as the target for miR-15b PMID: 23469855
  57. Bcl-2 protein is increased in cardiac tissue following exercise training in experimental diabetes. PMID: 22341695
  58. These results support the regulatory role of Bcl-2 in autophagy and define GABARAP as a novel interaction partner involved in this intricate connection. PMID: 24240096
  59. Upregulation of Bcl-2 in cerebellar granule cells contributes to the neuroprotective effect of mood stabilizer lamotrigine. PMID: 22564541
  60. RCAN1 induced the expression of the CREB target gene, Bcl-2. PMID: 23150431
  61. Folic acid supplementation can prevents Bcl-2 hypomethylation in rats with hyperhomocysteinemia, resulting in a decreased Bcl-2 expression. PMID: 22611939
  62. Lactuside B played a role in resisting cerebral ischemia by upregulating the expression of bcl-2 and downregulating bax expression. PMID: 22260021
  63. Lung injury induced by intestinal ischemia reperfusion is correlated with abnormal expression levels of Bcl-2 and Bax. Sodium aescinate treatment improves the ratio of Bcl-2/Bax which prevents lung injury. PMID: 22650024
  64. Fas and TNFR1 are expressed in the CA1 and CA3 regions after global ischemia/reperfusion in rats. Bcl-2 overexpression decreases the expression of Fas and TNFR1 and cell apoptosis after global IR. PMID: 21868309
  65. Lycopene counteracts the hepatic response to DMBA by altering the hepatic expression of bcl-2. PMID: 22978712
  66. An inverse correlation between Bcl-2 DNA methylation and Bcl-2 mRNA levels was observed in the tumors. PMID: 23354397
  67. Our results suggest that melatonin in these doses may provide modulation of bax and bcl-2 expression as well as bax/bcl-2 ratio to protect rat peripheral blood lymphocytes from gamma irradiation-induced apoptosis. PMID: 22982225
  68. Data suggest that expression of Bcl2 in distal colonic mucosa can be up-regulated by dietary fiber (here, by a grape antioxidant dietary fiber preparation). PMID: 22424444
  69. Breviscapin injection reduced delayed neuronal death, and reduced apoptosis of neuron after severe brain injury by improving the expression of Bcl-2 protein and inhibiting expression of Bax. PMID: 21845871
  70. Intermittent high glucose can lower Bcl-2/Bax ratio in INS-1 cells, and HO-1 may protect INS-1 cells against apoptosis possibly by up-regulating the Bcl-2/Bax ratio. PMID: 20965838
  71. Yikunning could increase the expressions of Bcl-2 and Bax in rat ovaries during perimenopause. PMID: 20684272
  72. Rb1 and Rg1 have protective effects on apoptosis of HK-2 cells by increasing the Bcl2/Bax ratio. PMID: 21158042
  73. Vitamin E can regulate Bcl2 and Bax expression in ovarian granulosa cells from aged rats. PMID: 21158059
  74. Bcl-2 and Bax protein expression may play an important role in the apoptosis of neuronal cells induced by benzo(a)pyrene. PMID: 22357517
  75. study suggests that low frequency and intensity ultrasound (LFU) can induce apoptosis in vitro and in vivo, and that three signaling proteins, caspase-3, Bcl-2, and survivin, might be involved in LFU-induced apoptosis PMID: 22819929
  76. findings highlight a new regulatory mechanism that Bcl-2 elevates ischemia-induced striatal neurogenesis by down-regulating expression of BMP-4 via activation of the Wnt/beta-catenin signaling pathway in adult rat brains PMID: 22521772
  77. FK506 has dramatically neuroprotective effects against the development of acrylamide neuropathy, which may be related to up-regulating the expression of HSP70 and Bcl-2, and down-regulating the expression of Bax. PMID: 20465949
  78. At low dosage, fipronil increases the apoptotic rates of PC12 cells possibly by decreasing the expression of Bcl-2 protein. PMID: 20465951
  79. Estrogen treatment up regulated the expression Bcl-x(L) and Bcl-2. PMID: 21710144
  80. beta-asarone can increase Bcl-2 level, finally interfere with the functions of Beclin 1 during the execution of autophagy. PMID: 22306244
  81. Subacute melamine treatment could induce a significant alteration of apoptosis Bcl-2, Bax and Caspase-3 mRNA expression in rat kidney tissue. PMID: 21126425
  82. Lymphostatic encephalopathy following the blockade of cervical lymphatics result in changes in bcl-2 and bax expression in the hippocampus, and that apoptosis is the main form of neuron death. PMID: 15719136
  83. Transcription of bcl-2 is possibly regulated by calmodulin I hypertrophic rat hearts, and CREB phosphorylation seems to be a minor factor in bcl-2 transcriptional regulation. PMID: 16344898
  84. IC-IGF-1 induces Bcl-2 expression in epithelial cells via IGF-1R and epidermal growth factor receptor pathways, and targeting IC-IGF-1 could be beneficial to treat chronic airway diseases. PMID: 22461702
  85. A combination of NGF and insulin local application can enhance the angiogenesis of the burn wound in diabetic rats and accelerate wound healing by increasing the expression of Bcl-2 and decreasing the expression of Bax. PMID: 21500593
  86. regulation of mitochondrial glutathione transport is a principal mechanism by which Bcl-2 suppresses mitochondrial oxidative stress PMID: 22115789
  87. In isolated rat hearts the expression of Bcl-2 increased significantly while that of Bax decreased in the sevoflurane postconditioning group versus the ischemia/reperfusion group. PMID: 22094093
  88. Vitexin exhibits apoptosis of cardiac muscle cell via up-regulating protein expression of Bcl-2 as well as down-regulating Bax and NF-kappaBp65. PMID: 22001368
  89. The decrease in DA content in the striatum may correlate with the abnormal behavior in elderly rats, which could be ascribed to the variations in Bad and Bcl-2. PMID: 21818658
  90. Melatonin can protect nerve cell against deltamethrin induced brain injury by inhibiting nerve cell apoptosis, downregulating expression of cytochrome C and upregulating expression of bcl-2. PMID: 18724894
  91. This study evaluated whether overexpressed Bcl-2 influences neurite outgrowth in immortalized hippocampal progenitor cells. PMID: 21916846
  92. up-regulated expression of bax and down-regulated expression of bcl-2 might play a key role in the increased apoptosis in the OB of the CUMS rats. PMID: 21903151
  93. Data suggest that it is possible that activation of p38/MAPK as well as reduction in Bcl2 lead to caspase activity and cell death. PMID: 21858143
  94. Scutellarin benzyl ester has a protective effect on acute myocardial hypoxic injury by downregulating Bcl-2 and Bax expression. PMID: 21672380
  95. Obtained data testify an active role of p53 and Bcl-2 peptides in regulation of neuronal activity in hypothalamus at change of a cycle wakefulness-sleep. PMID: 22145321
  96. The fish oil-treated group exhibited a reduction of Bcl-2 immunopositivity (p<0.05). PMID: 18614344
  97. The role of P53 and Bcl-2 proteins in 7, 12-dimethylbenz-(a)-anthracene-induced tumor growth. PMID: 22023760
  98. The BNP preconditioning can decrease the myocardial apoptosis induced by ischemia-reperfusion injury, which may be associated with an elevated expression of Bcl-2, an increased ratio of Bcl-2/Bax and a lowered expression of Bax. PMID: 21223820
  99. These results indicated that aucubin can obstruct H(2)O(2)-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation. PMID: 21120620
  100. results suggest that neuroprotection effects of hypothermia on injured developing rat brain may associate with enhanced generation of neuronal cells and Bcl-2-mediated reduction of apoptosis of these cells PMID: 21300124

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Subcellular Location Mitochondrion outer membrane, Single-pass membrane protein, Nucleus membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein
Protein Families Bcl-2 family
Tissue Specificity Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, an
Database Links

KEGG: rno:24224

STRING: 10116.ENSRNOP00000003768

UniGene: Rn.9996

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