Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit

Instructions
Code CSB-E08854r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name B-cell CLL/lymphoma 2
Alternative Names Bcl2 ELISA kit; Bcl-2Apoptosis regulator Bcl-2 ELISA kit
Abbreviation BCL2
Uniprot No. P49950
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 80-95  
1:2 Average % 95  
Range % 85-99  
1:4 Average % 95  
Range % 86-102  
1:8 Average % 98  
Range % 82-104  
Recovery
The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 85-100  
EDTA plasma (n=4) 95 87-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.745 2.768 2.757 2.572  
10 2.176 2.165 2.171 1.986  
5 1.485 1.463 1.474 1.289  
2.5 0.896 0.864 0.880 0.695  
1.25 0.569 0.543 0.556 0.371  
0.625 0.425 0.439 0.432 0.247  
0.312 0.296 0.284 0.290 0.105  
0 0.185 0.184 0.185    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
4.3 - 3 reviews

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Sample type: Serum

Sample species: Rat

Review: Serum Bcl-2 activity in a different group. N ¼ 8, p<0.05 for the group versus control group, &p <0.05 for the group versus DM group, #p <0.05 for the group versus CI group, $p <0.05 for the group versus DMCI group.

By Anonymous

Sample type: Tissue homogenate(intestine)

Sample species: Rat

Review: Bcl-2/Bax ratio content on the intestinal mitochondrial fraction before and after treatment with the specially formulated chamomile extract (100 and 300 mg/kg, twice daily, po) or 1% methylcellulose (vehicle). Results are represented as mean ± s.e.m (n = 10).

By Anonymous

Sample type: Tissue homogenate(colon tissue)

Sample species: Rat

Review: B-cell lymphoma-2 (BCL-2) in AA-induced UC (ulcerative colitis) in rats. Data are expressed as mean ± SD (n = 4). Statistical analysis was performed using ordinary one-way ANOVA.

By Anonymous

Target Data

Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (By similarity).
Gene References into Functions
  1. The findings suggest that experimental hyperthermia (EH) exposure leads to simultaneous activation of molecular switches of apoptosis (BCL2 and BAD) in cells of the follicular epithelium of the ovaries on days 3 and 4 after EH. PMID: 29658076
  2. miRNA-15b deteriorates cardiomyocyte apoptosis by post-transcriptionally downregulating the expression of Bcl-2 and MAPK3. PMID: 28814571
  3. Melatonin, but not curcumin, significantly increased the Bcl-2 expression of the hippocampal region. There was a significant correlation between SIRT2 and MDA levels. Results suggest that melatonin may increase cell survival in the hippocampus via decreasing oxidative stress and SIRT2 expression and increasing Bcl-2 expression. PMID: 29325994
  4. The expression of Bax and Bcl-2 after ligation of the parotid gland is closely related to the process of the parotid gland atrophy. PMID: 29243771
  5. Data suggest that neuroprotection via ghrelin involves up-regulation of Bcl-2 (B cell lymphoma 2 associated oncogene) and p-62/Sqstm1 (ubiquitin-binding protein p62) plus down-regulation of LC3-II (microtubule-associated protein 1 light chain 3) and Beclin-1. Studies were conducted in adult hippocampal neural stem cells in primary culture. PMID: 29057768
  6. Treatment of ellagic acid resulted in increased neuron viability, cell nuclear integrity, and the ratio of Bcl-2/Bax expression in the primary cultured neuron model. PMID: 28388366
  7. High expression of prosurvival BCL2 proteins and inhibition of apoptosis and autophagy prolongs Sertoli survival upon exposure to stress stimuli. PMID: 28379541
  8. Changes in p53 and Bcl-2 expression are spatially correlated to degenerated hair cells in aging rat cochleae. PMID: 28093932
  9. miR-486 may regulate cardiomyocyte apoptosis via p53-mediated BCL-2 associated mitochondrial apoptotic pathway. PMID: 28486954
  10. These data validated that SRF responded to hypoxia, which subsequently was involved in pulmonary hypertension by abnormally promoting viability of PASMCs via modulating expression of Bcl-2. PMID: 28176371
  11. upon CHIP knockdown, the expression of Bax and caspase-3 mRNAs increased even further, and the expression of Bcl-2 mRNA was further suppressed. The expression of the phosphorylated p65 and p38 proteins (p-p65 and p-p38) was also further enhanced. Thus, CHIP is a potent cardioprotective molecule. PMID: 28257878
  12. These findings indicated for the first time that pretreatment of H9c2 cells with catalpol can be against H2O2-induced apoptosis, and the protective effect of catalpol involves the mitochondrial-dependent caspase pathway and is associated with increased Bcl-2 and decreased Bax expression. PMID: 27166426
  13. Acute ethanol exposure induced autophagy-mediated heart toxicity and injury mainly through the ROS-JNK-Bcl-2 signaling pathway. PMID: 28369910
  14. Bcl2 protein content of skeletal muscle was increased after 8 weeks of treadmill running in aging rats. PMID: 27526155
  15. Sphallerocarpus gracilis polysaccharide protected pancreatic beta-cells from alloxan damage by several possible mechanisms, including: (1) repairing free radical damage; (2) reducing the apoptosis of pancreatic beta-cells by inhibiting the activities of caspase-3 and bax, and enhancing the activity of bcl-2; (3) stimulating insulin secretion and upregulating the pancreatic and duodenal homeobox 1 gene PMID: 27645924
  16. Data suggest that microRNA miR-98 might promote chondrocyte apoptosis and cartilage degradation by down-regulating Bcl-2 expression in the pathogenesis of osteoarthritis (OA), and that miR-98 can be a potential target for the treatment of OA. PMID: 27590063
  17. Morin-5'-sulfonic acid sodium salt prevents changes in the expression of apoptosis related proteins caspase 3, Bax and Bcl 2 due to mercury induced oxidative stress. PMID: 27919736
  18. Chrysin prevents apoptosis by upregulating the Bcl-2 mRNA expression and downregulating the pro-apoptotic (Bax, Bad) mRNAs in 3-nitropropionic acid induced behavioral despair/mitochondrial dysfunction/striatal apoptosis. PMID: 27690136
  19. Morinda citrifolia augmented the expression of Bcl2 in the skeletal muscle of intra-nigrally rotenone-infused Parkinsonian rats PMID: 26697948
  20. Hypoxia response element Bcl2 inhibition can effectively attenuate hypoxia-induced apoptosis resistance in pulmonary microvascular endothelial cells by downregulating Bcl-2 expression and upregulating caspase-3 and P53 expression. PMID: 26456506
  21. VPA protects myocardial cells from hemorrhagic and hypoxic stress through the Akt/BCL-2 survival pathway, indicating a potential use of HDACIs for acute severe hemorrhage treatment. PMID: 26886004
  22. TP53, BCL-2, and VEGFA genes may participate in the proliferation of esophagus cancer cells in a synergistic manner PMID: 26439224
  23. The spinal cord may be protected from spinal cord ischemia reperfusion injury by inhibition of BAX and activation of BCL2. PMID: 26400280
  24. Intermittent hypobaric hypoxia preconditioning activated the expression of neuroglobin and Bcl-2 in the rat hippocampal CA1 area following ischemia-reperfusion, and prevented hippocampal neuronal apoptosis. PMID: 26400308
  25. Pre-ischemic exercise prevented apoptosis in hippocampal CA3 neurons after cerebral ischemia by attenuating Bax/Bcl-2 protein ratio. PMID: 26012958
  26. MicroRNA34a induces apoptosis in PC12 cells by reducing expression of BCL2 and SIRT1. PMID: 26252661
  27. ISO increased NOXs-derived ROS which activated nuclear translocation of calpain-2, subsequently nuclear calpain-2 degraded CaMK II dB which reduced the ratio of Bcl-2 to Bax and mitochondria apoptosis pathway was triggered in rat cardiomyocytes PMID: 25820554
  28. The present study aimed to study the changes in P53, Bcl-2 and CD68 expression in response to amethopterin-induced lung injury and ameliorating the role of l-carnitine. PMID: 24986327
  29. Compared with the control group, the model and treatment groups showed significantly upregulated caspase-3, Bcl-2, and Bax mRNA and protein levels in brain tissues, but remarkably downregulated Bcl-2 mRNA and protein levels PMID: 26125843
  30. Bcl-2 expression is increased during neuronal differentiation of neural stem cells. PMID: 24986006
  31. Findings indicate that Bcl-2 phosphorylation and thereby Bcl-2/Beclin1 complex disruption play a crucial role in triggering autophagy and reducing mitochondrial damage in RIC rats after cerebral ischemia and require the involvement of the AKT activation. PMID: 25744447
  32. ROS/Bcl-2/Bax and the ROS/microcirculation/ starving pathway control the effect of scutellarin on type II diabetes mellitus-induced testicular damages PMID: 25861655
  33. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1alpha activity. PMID: 26319553
  34. DSW drinking reduced ulcer area as well as apoptotic signaling in acetic acid-induced duodenal ulcers. DSW, which contains selenium, provides intestinal protection against duodenal ulcers through the upregulation of Bcl-2 and thioredoxin reductase PMID: 24984066
  35. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis;tissues with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. PMID: 26003800
  36. Bcl-xL and Bcl-2, in a PI3K-dependent manner, which is upstream of NF-kappaB activation. PMID: 25220584
  37. The level of Bax mRNA and protein increased, while the content of Bcl2 mRNA and protein decreased 30 min after tetanization of the CA1 field of rat hippocampal slices. PMID: 25403402
  38. It has been found that NaBut reduces proliferation rate of ERasBcl (E1A, cHa-ras and bcl-2 ) cells significantly weaker than of ERas transformed cells PMID: 26035971
  39. up-regulated SSTR1 contributed to neuronal apoptosis after intracerebral hemorrhage, which was accompanied with reduced expression of bcl-2. PMID: 25035058
  40. oxymatrine can attenuate the development of UC by regulating the DOR-beta-arrestin1-Bcl-2 signal transduction pathway. PMID: 25480575
  41. the effects of mobile phones on the Bcl-2 gene and p53 proteins in rat brains PMID: 24763879
  42. Overexpression of Bcl-2 in the transplanted NESCs enhanced differentiation. PMID: 23324128
  43. Which might result in the disassociation of Bcl-2 from the Bad/Bcl-2 complex. PMID: 24957688
  44. Studied the effect of acute renal ischemia reperfusion on brain tissue and results showed decreased Bcl-2 expression and upregulated expression of NF-kappaB. PMID: 25063066
  45. Treatment with 6-OHDA reduced PC12 Bcl-2 mRNA levels by about fourfold. CDNF pretreatment attenuated this. PMID: 24633814
  46. Hepatocyte apoptosis could promote NAFLD progression; Fas, FasL, and Caspase-8 mRNA activation were important contributing factors to NAFLD. The upregulation of Bax and Bcl-2 expression might be one important mechanism of the apoptosis in NAFLD. PMID: 24938610
  47. results indicated that after TBI in rats CXCL12 combing CXCR4 receptors could inhibit the caspase-3 pathway by upregulating Bcl-2:Bax ratio, which protect neurons from apoptosis PMID: 23984821
  48. These results suggest the involvement of Bcl-2 and BDNF in processes of adaptation to SH and compensation of its damaging effects. PMID: 25282819
  49. Suggest that miR-21 promotes the development of heart failure with preserved left ventricular ejection fraction by up-regulating the expression of anti-apoptotic gene Bcl-2 and thereby promoting cardiac fibrosis. PMID: 24551276
  50. A critical role of NF-kappaB-mediated miR-30b modulation in Ang II-stimulated cardiomyocytes targeting Bcl-2. PMID: 24178239

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Subcellular Location Mitochondrion outer membrane, Single-pass membrane protein, Nucleus membrane, Single-pass membrane protein, Endoplasmic reticulum membrane, Single-pass membrane protein
Protein Families Bcl-2 family
Tissue Specificity Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, an
Database Links

KEGG: rno:24224

STRING: 10116.ENSRNOP00000003768

UniGene: Rn.9996

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