Rat Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit

Code CSB-E07395r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This Rat CD40LG ELISA Kit was designed for the quantitative measurement of Rat CD40LG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 ng/mL-120 ng/mL and the sensitivity is 0.47 ng/mL.

Target Name CD40 ligand
Alternative Names Cd40lg ELISA Kit; Cd40l ELISA Kit; Tnfsf5CD40 ligand ELISA Kit; CD40-L ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit; CD antigen CD154) [Cleaved into: CD40 ligand ELISA Kit; membrane form; CD40 ligand ELISA Kit; soluble form ELISA Kit; sCD40L)] ELISA Kit
Abbreviation CD40LG
Uniprot No. Q9Z2V2
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.87 ng/mL-120 ng/mL
Sensitivity 0.47 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %95
Range %83-98
1:2Average %93
Range %87-98
1:4Average %91
Range %83-95
1:8Average %103
Range %95-107
Recovery
The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8882-92
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1202.487 2.688 2.588 2.437
601.734 1.643 1.689 1.538
301.049 1.106 1.078 0.927
150.674 0.685 0.680 0.529
7.50.442 0.457 0.450 0.299
3.750.314 0.327 0.321 0.170
1.870.236 0.245 0.241 0.090
00.150 0.151 0.151
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Cytokine that acts as a ligand to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B. Induces the activation of kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.; Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Gene References into Functions
  1. Transient middle cerebral artery occlusion (MCAO) rats yielded an acceleration of thrombus formation that was accompanied by increased CD40 levels in serum. The brain infarction was significantly decreased in CD40 antagonist treatment group compared to MCAO model group. The mTOR/S6K signaling was activated in MACO model than that of CD40 antagonist treatment group. PMID: 29843579
  2. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in focal segmental glomerulosclerosis. PMID: 29155846
  3. Suggest that CD154-CD40 T-cell co-stimulation pathway is a key mechanism in kidney ischemia-reperfusion injury. PMID: 25993320
  4. These data suggest that placental ischemia-induced T-cell communication via the CD40L is one important mechanism leading to much of the pathophysiology of preeclampsia. PMID: 26310940
  5. PSB33 functions in the maintenance of photosystem II-light-harvesting complex II.[PSB33] PMID: 25511433
  6. Data suggest that astrocytes activated by CD40-CD40L in co-culture induce inflammatory cytokine production, and secreted cytokines re-activate astrocytes via Jak/STAT1701 pathways, and release more cytokines that contribute to the development of EAE. PMID: 21410936
  7. Monoclonal antibodies are immunosuppressive in pig to rat islet xenograft. PMID: 15665763
  8. B cells play a cooperative role via CD40L-CD40 interaction in T cell-mediated experimental autoimmune neuritis of Lewis rats PMID: 17188497
  9. HO-1 mitigated CD40L transcription in the xenograft and recipient spleen. PMID: 17976119
  10. Generated and characterized a novel anti-rat CD40L antibody with inhibitory activities. PMID: 18384807
  11. Adenovirus-mediated Cd40lg gene therapy induced both humoral and cellular immunity against rat model of hepatocellular carcinoma. PMID: 19016771
  12. Primary tumor cells obtained from MNU-induced mammary carcinomas show immune heterogeneity which can be modulated by low-efficiency transfection of CD40L gene. PMID: 19164932

Show More

Hide All

Subcellular Location Cell membrane; Single-pass type II membrane protein. Cell surface.; [CD40 ligand, soluble form]: Secreted.
Protein Families Tumor necrosis factor family
Database Links

KEGG: rno:84349

STRING: 10116.ENSRNOP00000001162

UniGene: Rn.44218

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1