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| Code | CSB-E07395r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 83-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 83-95 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 95-107 | |||||
| The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat Soluble Cluster of differentiation 40 ligand (sCD40L) ELISA Kit is a quantitative sandwich assay designed for the accurate detection and measurement of CD40 ligand protein in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat) samples.
CD40 ligand (CD40L)is a proteinthat plays a crucial role in the immune system by providing signals for the activation of antigen-presenting cells, and its dysregulation has been linked to several immune disorders.CD40L protein plays an important role in immune response and is a prime target for inflammation. This ELISA kit provides a reliable tool for the quantitative measurement of sCD40L in Rat samples, with a detection range of 1.87 ng/mL-120 ng/mL and a sensitivity of 0.47 ng/mL.
The assay is easy to use, with a total assay time of 1-5 hours and a sample volume of 50-100ul. The detection wavelength is 450 nm, making it compatible with most ELISA plate readers.
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