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| Code | CSB-E07346r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-103 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:4 | Average % | 108 | ||||
| Range % | 98-112 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 80-92 | |||||
| The recovery of rat HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-94 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This rat hepatocyte growth factor (HGF) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat HGF in multiple samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of HGF bound in the initial step.
HGF is synthesized by stromal and mesenchymal cells and functions as a pleiotropic cytokine on cells of mainly epithelial origin. It regulates cell motility and morphogenesis, induces angiogenesis, and promotes cell proliferation and migration by activating a tyrosine kinase signaling cascade after engagement to the proto-oncogenic c-Met receptor. The HGF-Met signaling pathway contributes to the development of epithelial organs in a paracrine manner, exerts regenerative effects on the epithelium, and facilitates the regression of fibrosis in numerous organs. The signaling of HGF through Met is associated with the biology of cancer types, neurons, and immunity. HGF is known to promote tumor progression and inhibit the development of fibrosis after tissue injury.
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