Rat hepatocyte growth factor,HGF ELISA kit

Instructions
Code CSB-E07346r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name hepatocyte growth factor (hepapoietin A; scatter factor)
Alternative Names DFNB39, F-TCF, HGFB, HPTA, SF, OTTHUMP00000206710|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor|lung fibroblast-derived mitogen
Abbreviation HGF
Uniprot No. P17945
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 93-103  
1:2 Average % 94  
Range % 91-97  
1:4 Average % 108  
Range % 98-112  
1:8 Average % 87  
Range % 80-92  
Recovery
The recovery of rat HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 86-94  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.185 2.284 2.235 2.061  
5 1.703 1.802 1.753 1.579  
2.5 1.276 1.295 1.286 1.112  
1.25 0.903 0.894 0.899 0.725  
0.625 0.650 0.641 0.646 0.472  
0.312 0.411 0.402 0.407 0.233  
0.156 0.319 0.312 0.316 0.142  
0 0.173 0.174 0.174    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity).
Protein Families Peptidase S1 family, Plasminogen subfamily
Database Links

KEGG: rno:24446

STRING: 10116.ENSRNOP00000009764

UniGene: Rn.10468

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.