Rat hepatocyte growth factor,HGF ELISA kit

Code CSB-E07346r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P17945
Lead Time 3-5 working days
Abbreviation HGF
Target Name hepatocyte growth factor (hepapoietin A; scatter factor)
Alias DFNB39, F-TCF, HGFB, HPTA, SF, OTTHUMP00000206710|fibroblast-derived tumor cytotoxic factor|hepatocyte growth factor|lung fibroblast-derived mitogen
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms.
HGNC 4893
RGD 2794
MGI 96079
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 93-103  
1:2 Average % 94  
Range % 91-97  
1:4 Average % 108  
Range % 98-112  
1:8 Average % 87  
Range % 80-92  
Recovery
The recovery of rat HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 86-94  
EDTA plasma (n=4) 95 91-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.185 2.284 2.235 2.061  
5 1.703 1.802 1.753 1.579  
2.5 1.276 1.295 1.286 1.112  
1.25 0.903 0.894 0.899 0.725  
0.625 0.650 0.641 0.646 0.472  
0.312 0.411 0.402 0.407 0.233  
0.156 0.319 0.312 0.316 0.142  
0 0.173 0.174 0.174    
References
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Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (By similarity).
Protein Families Peptidase S1 family, Plasminogen subfamily
Database Links

KEGG: rno:24446

STRING: 10116.ENSRNOP00000009764

UniGene: Rn.10468

Pathway MAPK signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Rap1 signaling pathway

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