Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Hgf

Uniprot No.

P17945

Research Area

Others

Alternative Names

HgfHepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

477-728aa

Target Protein Sequence

TIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

55.0kDa

Protein Length

Partial

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant rat hepatocyte growth factor (HGF) is a high-purity protein with purity levels greater than 90%, as verified by SDS-PAGE. This protein, derived from rat species and expressed in E. coli, includes the partial expression region 477-728aa. It features an N-terminal GST-tag for ease of purification and detection. It is available in both liquid and lyophilized powder forms.

Rat HGF is a crucial cytokine involved in liver regeneration and hepatocyte proliferation. HGF is produced by non-parenchymal cells in the liver, such as Kupffer cells and hepatic stellate cells, and its expression is significantly upregulated during liver regeneration, particularly following partial hepatectomy [1]. When stimulated by c-Met, HGF activates multiple downstream signaling cascades, including the PI3K/Akt and MAPK pathways, essential for hepatocyte proliferation and survival [2][3].

In vitro studies have demonstrated that HGF promotes hepatocyte proliferation and enhances their survival by exerting anti-apoptotic effects [4]. HGF also influences the differentiation of stem cells into hepatocyte lineages, indicating its pivotal role in liver development and repair processes [5].

References:
[1] M. Li, X. Zhou, J. Mei, X. Geng, Y. Zhou, W. Zhang, et al., Study on the activity of the signaling pathways regulating hepatocytes from g0 phase into g1 phase during rat liver regeneration, Cellular & Molecular Biology Letters, vol. 19, no. 2, 2014. https://doi.org/10.2478/s11658-014-0188-2
[2] A. Coutant, C. Rescan, D. Gilot, P. Loyer, C. Guguen‐Guillouzo, & G. Baffet, Pi3k-frap/mtor pathway is critical for hepatocyte proliferation whereas mek/erk supports both proliferation and survival, Hepatology, vol. 36, no. 5, p. 1079-1088, 2002. https://doi.org/10.1053/jhep.2002.36160
[3] H. Moteki, M. Kimura, & M. Ogihara, Activation of extracellular-signal regulated kinase by epidermal growth factor is potentiated by camp-elevating agents in primary cultures of adult rat hepatocytes, Biological and Pharmaceutical Bulletin, vol. 34, no. 10, p. 1542-1552, 2011. https://doi.org/10.1248/bpb.34.1542
[4] Z. Li, S. Mizuno, & T. Nakamura, Antinecrotic and antiapoptotic effects of hepatocyte growth factor on cholestatic hepatitis in a mouse model of bile-obstructive diseases, Ajp Gastrointestinal and Liver Physiology, vol. 292, no. 2, p. G639-G646, 2007. https://doi.org/10.1152/ajpgi.00292.2006
[5] S. Oh, M. Miyazaki, H. Kouchi, Y. Inoue, M. Sakaguchi, T. Tsujiet al., Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro, Biochemical and Biophysical Research Communications, vol. 279, no. 2, p. 500-504, 2000. https://doi.org/10.1006/bbrc.2000.3985

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I am interested in purchasing all of your in-stock quantity. Are you able to perform endotoxin-removal on this protein if you resuspend it? If so, what would be the additional lead time? Also, what is the expected yield of the protein after QC-testing and (potentially) endotoxin removal?

Very nice to receive your inquiry. If you request endotoxin removal, we can meet your demand, pls remark your requirement when placing the order.
And we recommend you to choose liquid form when requesting endotoxin removal, and it's better to choose aseptic processing along with endotoxin removal, this is based on the standpoint of the client.
If only choosing Endotoxin removal without aseptic processing, if bacteria are contained, and when bacterial autolysis encountered, endotoxins will surely be produced again,
so we suggest that the removal of endotoxin should be required with aseptic treatment at the same time.
However, if you insist and can be responsible for the result without aseptic processing, , it is also possible to remove the endotoxin without aseptic operation.
What is the expected yield of the protein after QC-testing and (potentially) endotoxin removal?
Different proteins may have different degrees of loss, There is no conclusive, but in general, the final amount is definitely above 1mg. If you order 1mg or less, the lead time is still 3-7 working days;
If you need 5mg the lead time will be longer.

Target Background

Function

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.

Gene References into Functions

HGF significantly mitigated the severity of pulmonary artery hypertension (PAH) and inhibited inflammation by attenuating the NF-kB signaling in the lungs of PAH rats. PMID: 29442198
Our results suggest that hUCMSCs can promote ovarian expression of HGF, VEGF, and IGF-1 through secreting those cytokines, resulting in improving ovarian reserve function and withstanding ovarian senescence. PMID: 28279229
eIf2alpga expression was upregulated during endoplasmic reticulum stress and interfering eIf2alpha expression proportionately down-regulated HGF expression. PMID: 28844859
VEGF treatment increases expression of HGF in vitro and in vivo and that, based on a series of integrative studies, we suggest that the effects of VEGF are mediated through downstream HGF signaling. PMID: 27036872
Hepatocyte growth factor (Hgf) plays a crucial role in the formation of the muscular diaphragmatic components by regulating the migration of myogenic progenitor cells into the primordial diaphragm. PMID: 27480986
Mesenchymal stem cells restore lung vascular permeability and alleviate lung injury in part by maintaining HGF levels. PMID: 27129877
The mechanism of antifibrogenic action of OBE is hypothesized to proceed via scavenging free radicals and activating liver regeneration by induction of HGF. PMID: 26213907
Data show that secretion of hepatocyte growth factor (HGF) was elevated, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta1 (TGF-beta1) levels were decreased after infusion of mesenchymal stem cells. PMID: 25736907
HGF induction is a crucial contributor to the acceleration of cell dedifferentiation and growth. PMID: 25793303
HGF was hardly detectable in normal tympanic membrane; however, a significant increase was noted in its expression starting from the third day after injury throughout the follow-up period, with the highest level on day 05. PMID: 25920966
p27 haploinsufficiency protects against heart injury by VEGF/HGF mediated cardioprotection and increased angiogenesis through promoting IKK activation. PMID: 25099287
spleen seemed to exhibit an inhibitory effect on liver regeneration by downregulating HGF and its receptor c-Met in the liver PMID: 25862490
Mesenchymal stromal cells increased serum and tissue HGF levels, Met tubular expression and prevented the suppression of tubular MSP/RON expression. PMID: 25277788
Hepatocyte growth factor upregulates nexilin gene expression in cardiomyocytes via JNK pathway. PMID: 25062485
Studied the preventive and control effect of matrine on transforming growth factor (TGF-beta1) and hepatocyte growth factor (HGF) of liver fibrosis tissue in rats. PMID: 25063067
Transplanted mesenchymal stem cells expressing human HGF combined with G-CSF potentially offer synergistic therapeutic benefit for the treatment of pulmonary arterial hypertension. PMID: 23933910
cytoskeletal rearrangement and cell-cell adhesion, such as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of HGF on the blood-brain barrier PMID: 24370951
these results indicate that HGF activates the cMetAktTGIF signaling pathway, inhibiting CF proliferation and transformation in response to TGFb1 stimulation. PMID: 24840640
Fibroblasts activated by hepatoma cell supernatant express COX-2 and HGF at higher levels. PMID: 24815169
HGF/c-Met signaling pathway plays an essential role in the homing of MSCs towards injured liver triggered by intestinal ischemia-reperfusion, and then mediates MSC-induced liver repair. PMID: 24782653
results indicated that HGF, MMP-9 and TGF-beta1 may participate in the formation and prognosis of hydrocephalus. PMID: 23270462
the results provide evidence that HGF can accelerate reepithelialization in skin wound healing by dedifferentiation of epidermal cells in a manner related to the b1-integrin/ILK pathway. PMID: 24490163
This data indicates that hypoxic modulation of satellite cell-mediated angiogenesis involves a reduction in satellite cell HGF expression. PMID: 24122166
These results strongly suggest that the combined stimulation of IL-6 and glucocorticoids induces the activation of both Reg and HGF genes. PMID: 23954444
HGF is important in the repair of the dentine-pulp complex potentially participating in several aspects of wound healing. PMID: 23273597
HGF is a growth factor playing a key role in islet mass increase and hyperinsulinemia in diet-induced obesity rats; the HGF-Met axis may have a role on insulin signaling in the liver PMID: 23024263
HGF, bFGF and VEGF significantly increased the growth of vascular endothelial cells. PMID: 22361334
The renal fibrosis of CAAN could be protected by isogenic MSC transplantation, probably via upregulation of HGF and downregulation of TGF-beta1. PMID: 22661380
The antifibrotic effects of Astragalus mongholicus may be associated with up-regulation of HGF expression and down-regulation of TGF-beta1 expression in rats with unilateral ureteral obstruction. PMID: 19069655
HGF is a biomarker and mediator in cardioprotection and cardiovascular regeneration [review] PMID: 22318499
The data showed that the activation of hepatocyte growth factor/c-Met signaling is a major pathway involved type II cell proliferation induced by ultrafine carbon black. PMID: 22245998
Upregulation of miRNA-29 by HGF and downregulation by TGF-beta take part in the anti- or profibrogenic response of HSC, respectively PMID: 21931759
in the same stages, hepatocyte growth factor reduces the amount of actin present in the blood testis barrier region, in which occludin levels are highest and modifies the morphology of the actin cytoskeleton network PMID: 21843593
Hepatocyte growth factor-induced amelioration in renal interstitial fibrosis is associated with reduced expression of alpha-smooth muscle actin and transforming growth factor-beta1 PMID: 22165288
HGF and VEGF participate in the pathological injury and repair of cerebral tissue in rats with chronic hydrocephalus after subarachnoid hemorrhage. PMID: 21643626
Both HGF and glial cell line-derived neurotrophic factor (GDNF) have significant neurotrophic effects, but only HGF can promote neurogenesis, angiogenesis, and synaptogenesis and inhibit fibrosis in brains after transient middle cerebral artery occlusion. PMID: 20963849
Ezrin, HGF and C-met may have positive effects on the carcinogenesis of rat pancreas PMID: 21134835
VEGF and TGFbeta might modulate HGF signaling to influence route selection during cancer progression PMID: 21075102
HGF expression following sciatic nerve ligation was found decreased in ipsilateral dorsal root ganglions from day 3 to day 14. AND no significant change in HGF expression was detected in the lumbar spinal cords. PMID: 20814222
protective effects of GDNF and HGF on brain ischemia were associated with both antiapoptotic and antiautophagic effects; maybe two types of cell death can occur in the same cell at the same time, and GDNF and HGF are capable of ameliorating both pathways PMID: 20175208
HGF influences the metabolic activity of isolated Leydig cells (ie, uPA and MMP 2 secretion) and increases TGF-beta activity PMID: 19834131
HGF counteracted the effect of high glucose on protein kinase A and protein kinase G activity. Thus, inhibition of PKA and activation of PKG are involved in the antioxidant role of HGF. PMID: 20466058
Valsartan alleviates BLM-induced pulmonary fibrosis in rats, possibly by inhibiting the expression of TGF-beta and stimulating the expression of HGF in lung tissues. PMID: 16115399
HGF may play an important role in the early stages of dental pulp repair. PMID: 17294746
HGF-induced EPC proliferation is mediated partly via activation of STIM1 PMID: 20404049
HGF expression was significantly increased in control rats treated with Astragalus mongholicus compared to rats with unilateral ureteral obstruction. PMID: 19292056
Over-expression of HGF in vascular smooth muscle cells can be helpful for promoting endothelial stem cells differentiation, increasing their migration and proliferation PMID: 19095317
HGF is expressed in primary sensory neurons from dorsal root ganglia and spinal cord, and may be involved in sensory transmission. PMID: 11744165
Differential mitogenic effects of single chain hepatocyte growth factor (HGF)/scatter factor and HGF/NK1 following cleavage by factor Xa PMID: 11832492
results suggest that hepatocellular hypoxia causes inhibition of HGF-mediated proliferation PMID: 11839582

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Protein Families

Peptidase S1 family, Plasminogen subfamily

Database Links

KEGG: rno:24446

STRING: 10116.ENSRNOP00000009764

UniGene: Rn.10468

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