Product Details

Purity

Greater than 90% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Dog CD20 at 10 μg/mL can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC₅₀ is 147.3-199.0 ng/mL.

Target Names

MS4A1

Uniprot No.

Q3C2E2

Alternative Names

(Membrane-spanning 4-domains subfamily A member 1)(CD antigen CD20)

Molecular Characterization

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Source

Mammalian cell

Expression Region

1-297aa

Target Protein Sequence

MTTPRNSMSGTLPVDPMKSPTAMYPVQKIIPKRMPSVVGPTQNFFMRESKTLGAVQIMNGLFHIALGSLLMIHTDVCAPICITMWYPLWGGIMFIISGSLLAAADKNPRKSLVKGKMIMNSLSLFAAISGIIFLIMDIFNITISHFFKMENLNLIKAPMPYVDIHNCDPANPSEKNSLSIQYCGSIRSVFLGVFAVMLIFAFFQKLVTAGIVENEWKKLCSKPKSDVVVLLAAEEKKEQPIETTEEMVELTEIASQPKKEEDIEIIPVQEEEGELEINFAEPPQEQESSPIENDSIP

Mol. Weight

34.4 kDa

Protein Length

Full Length

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Dog CD20 (MS4A1) recombinant protein was produced in mammalian cell, where the gene sequence encoding dog MS4A1 (1-297aa) was expressed with the C-terminal 10xHis tag. The activity was measured by its binding ability in a functional ELISA. This dog MS4A1 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.

The canine CD20 (MS4A1) protein is composed of 297 amino acids and is about 84.6% homologous to human CD20 protein. Its structure is also similar to that of human CD20, consisting of four hydrophobic transmembrane domains, one intracellular domain and two extracellular domains. composition, the N- and C-termini located within the cell membrane.
CD20 is an important differentiation antigen of B cells and is expressed in pre-B cells, mature B cells and activated B cells. In addition, CD20 molecules on germinal center B cells also have high expression. Besides being expressed in normal B cells, CD20 is also expressed in tumor cells such as lymphomas and leukemias derived from B cells, as well as in B cells involved in immune diseases and inflammatory diseases. So the CD20 antigen has become a target for the treatment of lymphoma, leukemia, melanoma and some autoimmune diseases. Canine CD20 is expressed in PBMCs and lymph nodes of healthy dogs, B cells of canine lymphoma, but not T-cell lymphoma cells, non-T, and non-B-cell lymphoma cells.

Target Background

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR.

Gene References into Functions

Our findings indicate that CD47 blockade alone and in combination with anti-CD20 may be an effective strategy for treating canine lymphoma PMID: 27856424
Canine CD20 gene was cloned and sequenced, and the expression of CD20 mRNA was investigated in canine peripheral blood mononuclear cells (PBMCs), and lymph nodes from healthy dogs, and canine lymphoma cells. PMID: 16061290
This study examined the immunoreactivity of a monoclonal antibody to MUM1/IRF4 and compared it with expression of CD79a and CD20 in 109 plasmacytomas in 107 dogs. PMID: 18039900

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor.

Protein Families

MS4A family

Tissue Specificity

Expressed in PBMCs and lymph node from healthy dogs, in B-cells of canine lymphoma, but not in T-cell lymphoma cells and non-T and non-B-cell lymphoma cells.

Database Links

KEGG: cfa:485430

STRING: 9615.ENSCAFP00000035044

UniGene: Cfa.23836

Code	CSB-MP661636DO
Abbreviation	Recombinant Dog MS4A1 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$570
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Image	CSB-MP661636DO is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Dog CD20 at 10 μg/ml can bind Anti-CD20 recombinant antibody (CSB-RA015007MA3HU), the EC₅₀ is 147.3-199.0 ng/mL. Biological Activity Assay The purity of VLPs was greater than 90% as determined by SEC-HPLC
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Recombinant Dog B-lymphocyte antigen CD20 (MS4A1)-VLPs (Active)

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