Recombinant Macaca fascicularis CD44 antigen(CD44),partial (Active)

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Code CSB-MP4290MOV
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/ml can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC50 is 64.18-90.91 ng/mL. Biological Activity Assay
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Product Details

Description

This Macaca fascicularis CD44 recombinant protein was produced in Mammalian cell, where the gene sequence encoding Macaca fascicularis CD44 (21-650aa) was expressed with the C-terminal 10xHis tag. The purity of this CD44 protein was greater than 95% by SDS-PAGE. The activity was validated.

The main functions of CD44 protein can be summarized as: mediate the binding of lymphocytes to the high columnar endothelial cells in the post-capillary venules, so that the lymphocytes pass through the vessel wall and return to the lymphoid tissue; participate in the activation process of lymphocytes; interact with the extracellular matrix It binds to matrix molecules such as hyaluronic acid and laminin in the cytoskeleton; it binds to cytoskeletal proteins, participates in the formation of cell pseudopodia, and is related to the migration of cells. Through the detection of various CD44 molecules in malignant tumor tissues, it is found that many types of tumors express CD44 molecules to varying degrees, and are closely related to tumor growth, development, metastasis and prognosis.

 

Some researchers compared the expression of CD44 isoforms containing exons V5 and V6 in human and Macaca fascicularis tissues. Cloning and sequencing of Macaca fascicularis CD44 exons V5 and V6 revealed 97% and 95% homology between human and monkey, respectively. Two monoclonal antibodies directed against the epitope encoded by human exon V5 and the epitope encoded by exon V6 were used to determine the expression of CD44 isoforms in humans and Macaca fascicularis. Immunohistochemical screening of representative features of normal human and Macaca fascicularis tissues revealed nearly identical expression of CD44 isoforms containing exons V5 and V6 in both species. These data suggest that Macaca fascicularis can be used as a system to test the efficacy of anti-mutant CD44 MAbs for antibody-based tumor therapy.

 

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/mL can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC50 is 64.18-90.91 ng/mL.
Target Names CD44
Uniprot No. A0A2K5VJE0
Alternative Names (Extracellular matrix receptor III)(GP90 lymphocyte homing/adhesion receptor)(HUTCH-I)(Hermes antigen)(Hyaluronate receptor)(Phagocytic glycoprotein 1)(Phagocytic glycoprotein I)
Molecular Characterization
Species Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Source Mammalian cell
Expression Region 21-650aa
Target Protein Sequence QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSVGFETCRYGFIEGHVVIPRIQPNSICAANHTGVYILTSNTSQYDTYCFNASAPPKEDCTSVTDLPNAFDGPITITIVNPDGTRYIKKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFHTFSTAHPIPDEDGPWITDSTDRIPATTLMSTSATATETATKRQETQDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRVFDHTKQNKDWTQWNPSHSNPEVLLQTTARITADVDRNGTTAHEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQAIPSSTTEETATQKEQWFGNRWHEGYLQTPREDSHSTTGTAAASALTSHPMQGRTTPSPEDSSWTDFFDPISHPMGRGHQAGRRMDMDSSHSTTLQPTADPNTGLVEDLDRTGPLSMTTKQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPDTKESRTFIPVTSAKTGSFGVTEVTVGDSNSNVNHSLSGDQDAFYPSGGSHTTHGSESAGHSHGSQEGGANTTSGPVRTPQIPE
Mol. Weight 71.6 kDa
Protein Length Partial
Tag Info C-terminal 10xHis-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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