Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/mL can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC₅₀ is 64.18-90.91 ng/mL.

Target Names

CD44

Uniprot No.

A0A2K5VJE0

Alternative Names

(Extracellular matrix receptor III)(GP90 lymphocyte homing/adhesion receptor)(HUTCH-I)(Hermes antigen)(Hyaluronate receptor)(Phagocytic glycoprotein 1)(Phagocytic glycoprotein I)

Molecular Characterization

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

21-650aa

Target Protein Sequence

QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSVGFETCRYGFIEGHVVIPRIQPNSICAANHTGVYILTSNTSQYDTYCFNASAPPKEDCTSVTDLPNAFDGPITITIVNPDGTRYIKKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFHTFSTAHPIPDEDGPWITDSTDRIPATTLMSTSATATETATKRQETQDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAGWEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPRVFDHTKQNKDWTQWNPSHSNPEVLLQTTARITADVDRNGTTAHEGNWNPEAHPPLIHHEHHEEEETPHSTSTIQAIPSSTTEETATQKEQWFGNRWHEGYLQTPREDSHSTTGTAAASALTSHPMQGRTTPSPEDSSWTDFFDPISHPMGRGHQAGRRMDMDSSHSTTLQPTADPNTGLVEDLDRTGPLSMTTKQSNSQSFSTSHEGLEEDKDHPTTSTLTSSNRNDVTGGRRDPNHSEGSTTLLEGYTSHYPDTKESRTFIPVTSAKTGSFGVTEVTVGDSNSNVNHSLSGDQDAFYPSGGSHTTHGSESAGHSHGSQEGGANTTSGPVRTPQIPE

Mol. Weight

71.6 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CD44 serves as a critical adhesion molecule mediating hyaluronate binding, cell migration, and tumor metastasis signaling, making it a high-value target in oncology and immune cell trafficking research. This cynomolgus monkey CD44 construct (aa 21–650) encompasses the extracellular and transmembrane domains, providing broad functional domain coverage suitable for antibody development, cell adhesion and spreading assays, and integrin-ligand binding studies. Mammalian cell expression preserves native glycosylation and folding, and validated binding activity confirms proper conformation: immobilized at 2 μg/mL, the protein binds an anti-CD44 monoclonal antibody with an EC50 of 64.18–90.91 ng/mL in functional ELISA, supporting use in antibody screening and cross-reactivity validation for preclinical primate models. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, this C-terminal His-tagged protein satisfies the quality criteria typical for cell migration and invasion assays as well as tumor metastasis research.

Code	CSB-MP4290MOV
Abbreviation	Recombinant Cynomolgus monkey CD44 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$190
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CD44 at 2 μg/ml can bind Anti-CD44 monoclonal antibody (CSB-MA004938A0m), the EC₅₀ is 64.18-90.91 ng/mL. Biological Activity Assay
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Recombinant Macaca fascicularis CD44 antigen (CD44), partial (Active)

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