Purity
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.②Recombinant Paguma larvata ACE2 protein (CSB-MP684964PAL) enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 μM.
Alternative Names
ACE-related carboxypeptidase
Molecular Characterization
Species
Paguma larvata (Masked palm civet)
Expression Region
18-740aa
Target Protein Sequence
QSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVT
Tag Info
C-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The ACE2 protein CSB-MP684964PAL is a recombinant Paguma larvata ACE2 produced in mammalian cells, with a C-terminal hFc-tag. Its expression region corresponds to amino acids 18-740 of the Paguma larvata ACE2 protein. This recombinant ACE2 protein is biologically active, low in endotoxin (less than 1.0 EU/µg protein, determined by the LAL method), and high in purity (greater than 90%, SDS-PAGE). In the functional ELISA, SARS-CoV-2 S-RBD can bind to this ACE2 protein binds to the anti-ACE2 antibody with an EC50 constant of 5.056-7.559 ng/ml. Its enzyme activity was determined by cleaving fluorogenic peptide substrate, with the Km of 22.84 nM. It is available now.
ACE2 is a zinc metalloenzyme and carboxypeptidase that negatively regulates the renin-angiotensin system (counterbalancing ACE activity), acts as the key SARS-CoV and SARS-CoV0-2 receptor, and stabilizes the transport of the neutral amino acids.
