ICOS Antibody

Datasheet
Code CSB-PA745834
Size US$167
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  • Western blot analysis of 3T3 lysis using ICOS antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100
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Product Details

Uniprot No. Q9Y6W8
Target Names ICOS
Alternative Names Activation inducible lymphocyte immunomediatory molecule antibody; Activation-inducible lymphocyte immunomediatory molecule antibody; AILIM antibody; CD278 antibody; CD278 antigen antibody; CD28-related Protein-1 antibody; CRP1 antibody; CVID1 antibody; ICOS antibody; ICOS_HUMAN antibody; Inducible costimulator antibody; Inducible T cell co stimulator antibody; Inducible T-cell costimulator antibody; MGC39850 antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Synthesized peptide derived from the Internal region of Human ICOS.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).
Gene References into Functions
  1. ICOS (-693A/G) gene polymorphism is associated with Colon Cancer Risk. PMID: 29580042
  2. The altered soluble (s)PD1 and sICOS serum levels in the different Hepatitis b (HBV) groups may reflect the dysregulation of T cell activation, and may be associated with the HBV pathological process. PMID: 28983583
  3. we have found an association between chronic spontaneous urticaria and polymorphisms in the ICOS gene. To our knowledge, this report is the fi rst to suggest and initially indicate a possible association of the ICOS rs6726035 TT genotype with CSU pathogenesis. PMID: 28940644
  4. Suggest that genetic polymorphisms of ICOS function as sex-dependent risk factors for development of acute rejection in an Iranian kidney transplant population. PMID: 28031007
  5. Binding of NUFIP2 to Roquin promotes recognition and regulation of ICOS mRNA. PMID: 29352114
  6. OX40 and ICOS act in a cooperative, nonredundant manner to maximize and prolong the T follicular helper cell response that is generated after acute virus infection PMID: 27895177
  7. High ICOS expression is associated with colorectal neoplasms. PMID: 27197182
  8. This is the first replication association study of rs4325730 upstream of ICOS with AIH in the Japanese population and rs4325730G is a risk allele. PMID: 27974812
  9. the induction pathway of ICOS(+) Foxp3(+) cells was analyzed PMID: 27756099
  10. ratio of ICOS(+) /PD-1(+) Tfh cells and the frequency of IL-21(+) Tfh cells may be indicators for evaluating the idiopathic membranous nephropathy development PMID: 26845249
  11. Follicular helper T cells differentiation is a multistage process involving BCL6 and other transcription factors, cytokines, and costimulation through ICOS and several other molecules. (Review) PMID: 26120879
  12. novel deletion mutation in Pakistani siblings presents in childhood with a combined Immunodeficiency associated with an enteritis, hepatitis and impaired antiviral immunity PMID: 26399252
  13. findings show Tregs from inflamed lung of sarcoidosis patients characterized by ICOS(high) phenotype; high-level ICOS expression was restricted to Tregs from inflamed lung and was absent in blood Tregs of sarcoidosis patients and in lung and blood Tregs of healthy volunteers; potential implication of ICOS/ICOS-L immune-regulatory axis in in disease activity and resolution PMID: 26415669
  14. findings indicated that circulating memory Tfh cells, especially CCR7+ICOS+ memory Tfh cells, may be associated with the relapse of MS and may serve as a new therapeutic target PMID: 26231034
  15. Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID: 25569260
  16. This study found that ICOS rs1559931 SNP was associated with decreased hepatitis B virus-related hepatocellular carcinoma risk in the studied Chinese Han population, except for patients with natural clearance of hepatitis B virus. PMID: 26074057
  17. Thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS. PMID: 25210803
  18. Data indicate that T cells redirected with an inducible T-cell costimulator ICOS-based chimeric antigen receptors CAR maintained a core molecular signature characteristic of TH17 cells. PMID: 24986688
  19. Increased numbers of circulating ICOS(+) and IL-21(+) Tfh and CD38(+) plasma cells may be exhibited by patients with recent diagnoses of primary biliary cirrhosis. PMID: 25404409
  20. Aberrant sPD1 and sICOS serum levels may reflect the dysregulation of Tcell activation, and are associated with the pathological injury of chronic HCV infection. PMID: 23426717
  21. Data indicate that Tregs and its inducible costimulatory molecule (ICOS+) subsets are decreased in patients with myocardial infarction (MI) and stable angina (SA). PMID: 22426168
  22. Data indicate the expression of programmed cell death protein 1 (PD-1), inducible T-cell Costimulator (ICOS), Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) and T cell immunoglobulin and mucin protein 3 (Tim-3) in T-cells from geriatric and younger subjects. PMID: 24083425
  23. The associations between costimulatory molecule gene polymorphisms including: CTLA4, PD-1, ICOS and CD28 with active cytomegalovirus (CMV) infection were evaluated in hematopoietic stem cell transplant patients. PMID: 24057239
  24. The acquisition of B cell stimulating properties by naive cord blood CD4 T cells required the STAT3-dependent expression of ICOS and IL-21. PMID: 23923047
  25. SNPs of ICOS showed no association with Hashimoto thyroiditis. PMID: 23138463
  26. In a transgenic graft-vs-host disease (GVHD)model, mouse CD4 T cells depend more heavily on ICOS/phosphatidylinositol 3-kinase (PI3K) signaling axis, whereas CD8 T cells are able to induce GVHD utilizing PI3K-independent ICOS signaling mechanisms. PMID: 23729441
  27. A molecular and computational diagnostic approach identifies FOXP3, ICOS, CD52 and CASP1 as the most informative biomarkers in acute graft-versus-host disease. PMID: 22491736
  28. Results suggest that the serum level of sICOS is increased in patients with diffuse cutaneous SSc and correlates with the severity and activity of skin sclerosis and interstitial lung disease. PMID: 23053221
  29. Augmented ICOS signalling may contribute to the pathogenesis of systemic sclerosis during early progressive disease. Soluble ICOS levels may be used as a serum marker for the activity and severity of systemic sclerosis. PMID: 23024058
  30. ICOS-Fc inhibited the adhesion of both dendritic cells to vascular and lymphoid endothelial cells, their migratory activity, and the expression of the Rac-1 activator beta-Pix involved in cell motility. PMID: 23275603
  31. ICOS expression on CD4 + CD45RO + and CD8 + CD4RO + T cells was significantly increased in systemic lupus erythmetaous (SLE) patients. ICOS expression in lupus nephritis patients was higher than in SLE patients without lupus nephritis. PMID: 21479882
  32. Genetic variation in ICOS gene is associated with acute rejection of liver transplantation. PMID: 22579879
  33. ICOS gene polymorphisms may affect the risk of breast cancer and show that some SNPs are associated with breast cancer characteristics in a northern Chinese population. PMID: 21917182
  34. The constellation of specific alleles in CTLA-4, CD28, and ICOS genes contributes to the susceptibility and clinical course of non-small-cell lung cancer. PMID: 21669243
  35. Increased quantities of inducible costimulator-positive Tregs may influence IgG4 production in IgG4-related autoimmune pancreatitis. PMID: 21926547
  36. NPM-ALK induces expression of the growth-promoting receptor ICOS. PMID: 21765024
  37. ICOS polymorphisms was significantly different in pemphigus and suggest that genetically determined abnormal function of costimulatory receptors in T cells may be associated with the pathogenesis of pemphigus PMID: 21084022
  38. circulating neoplastic T cells correspond more often to a CD10-positive subset than to an ICOS-positive subset in Angioimmunoblastic T-cell lymphoma PMID: 21499231
  39. This indicates that the investigated ICOS polymorphisms do not modulate the risk of B-CLL in the Polish population, but are associated with disease dynamics, in particular with the time to Rai stage progression. PMID: 21526489
  40. we found a trend for reduced expression of ICOS after gluten-free diet treatment in the intestine of celia disease children. PMID: 21288140
  41. polymorphisms of three major genes involved in the regulation of immune response, CTLA4, CD28 and ICOS might be involved in development of the clinical variables of IgAN PMID: 21677403
  42. these data suggest that preeclampsia is associated with ICOS, but is not associated with the CTLA-4 or CD28 gene polymorphisms. PMID: 21160481
  43. Report the role of ICOS in allergen-induced T cell activation in allergic asthma and rhinitis. PMID: 21356099
  44. these results suggest that the polymorphisms in the CD28, CTLA4 and ICOS genes at least do not modulate risk of melanoma and nor do those influence the disease prognosis in the investigated population. PMID: 19672595
  45. In active ulcerative colitis CD86 and ICOS were over-expressed in the intestinal epithelial cells and lamina propria mononuclear cells. PMID: 20388394
  46. Increased amount of CD86 or ICOS positive lamina propria mononuclear cells and enterocytes suggests that co-stimulatory molecules may play a role in the pathogenesis of Crohn disease. PMID: 20019769
  47. Results reveal a vital role for ICOS signaling in the generation and maintenance of human T(H)17 cells and suggest that components of this pathway could be therapeutically targeted to treat cancer or chronic infection. PMID: 20980695
  48. High ICOS expression is associated with lymphomas of T follicular helper cell. PMID: 20207847
  49. The rs6726035 in ICOS was significantly associated with RA, Comparing the genotype frequencies in the codominant model (CC vs. CT, CC vs. TT) in the rs6726035 SNP revealed that the C allele appeared to be decreased in patients with RA patients. PMID: 20113255
  50. ICOS polymorphisms were not associated with susceptibility to cervical squamous cell (CSCC) and with histologic grade of CSCC. PMID: 19913589
  51. These data suggest that amongst the three putative ICOS glycosylation sites, N89 is required for proper ICOS protein folding in the ER, intracellular trafficking and ligand binding activity. PMID: 19931508
  52. its polymorphism may contributes to the pathogenesis of spondyloarthropathy PMID: 11771526
  53. Genetic polymorphism of the human ICOS gene. PMID: 11904679
  54. role of ligation in modulating TCR-induced transcriptional profiles PMID: 12195015
  55. ICOS reversely regulates IL-10 on re-activation of human effector T cells with mature dendritic cells. Interaction of ICOS with ICOS-L strongly promoted IL-10 secretion. PMID: 12207353
  56. ICOS provides co-stimulation to memory t-cells [review] PMID: 12456021
  57. Analysis of the upstream region of ICOS revealed an additional eight single nucleotide polymorphism PMID: 12753665
  58. local immune responses may be modulated by H4/ICOS expressed on T cells in the joints of patients with rheumatoid arthritis, and thus it may be involved in the pathogenetic mechanism of rheumatoid arthritis. PMID: 12784384
  59. ICOS is upregulated on human T cells after stimulation and can modulate both Th1 and Th2 cytokine production in the absence and presence of B7-1 (CD80) and B7-2 (CD86)costimulation. PMID: 12864987
  60. Targeting of specific chemokine and chemokine receptor pathways and/or ICOS may have clinical application in the prevention and treatment of chronic allograft nephropathy. PMID: 12919091
  61. AILIM has a role in regulating the signaling cascades for T-cell proliferation and IL-10 production PMID: 14550257
  62. ICOS does not belong to the group of genes that, if mutated, present clinically as the hyper-IgM syndrome. PMID: 14610488
  63. Highly expressed ICOS in activated CD4(+) lamina propria T cells of inflammatory bowel (IBD) contributes to dysregulated immune responses. Hyperexpression limited to inflammatory sites in IBD. Feasible therapeutic target for treatment of IBD. PMID: 14988837
  64. A subtype of regulatory T cells in human blood can be identified by expression of ICOS after activation; these ICOS-expressing cells block the capacity of reciprocal ICOS-negative cells to proliferate and to produce cytokines. PMID: 15100277
  65. ICOS expression on activated Th cells may provide a powerful means to amplify effector T cell responses in peripheral tissues, independently of the polarized state of the Th cells PMID: 15265908
  66. AILIM/ICOS-B7h interactions play an important role in the endothelium in controlling the entry of memory/effector T-cells into inflamed tissues in the periphery. PMID: 15339883
  67. The incidence of ICOS deficiency among patients with Common variable immunodeficiency is less than 5% PMID: 15507387
  68. invariant NKT cell activation is regulated by CD28 and IOCS independently PMID: 15629449
  69. A predominant effect of ICOS-mediated costimulation on T helper type 2 (Th2) cell differentiation in transgenic ICOS-deficient mice may be achieved by the enhancement of IL-4 receptor-mediated signaling. PMID: 15699127
  70. ICOS is an influential costimulatory pathway in atherosclerosis & may play a protective role. It localizes with T cells in human aortic plaque. PMID: 15941568
  71. This study could not confirm association with the CD28/CTLA4/ICOS gene region in multiple sclerosis. PMID: 16005527
  72. Two variants in the ICOS promoter region are significantly associated with allergic sensitization and production of type 2 T helper (Th2) cell cytokines PMID: 16081771
  73. ICOS is involved in abnormal T cell activation in systsemic lupus erythematosus (SLE) and blockade of the interaction between ICOS and its receptor may have therapeutic value in the treatment of this intractable disease. PMID: 16563187
  74. There are no major effects of the CD28/CTLA4/ICOS gene region on susceptibility to primary sclerosing cholangitis but minor contributions cannot be excluded. PMID: 16638702
  75. ICOS activation is associated with T helper type 1 (Th1) cell responses in human intracellular tuberculosis infection and may contribute to the amplification of those responses. PMID: 16670305
  76. This review summarizes the evidence that ICOS expression regulates T-cell-dependent B cell responses and proposes a model for the role of ICOS in diseases characterized by dysregulated humoral immunity. PMID: 16790364
  77. genetic variation in ICOS gene regulates the expression of both CTLA4 and ICOS and not only the splicing of sCTLA4 as suggested earlier. PMID: 16996590
  78. Both the PI3-kinase/Akt and Rho family cascades operate coordinately to induce the forward migration of activated T cells by AILIM/ICOS signaling. PMID: 17077177
  79. With multiethnic DNA panels that represent a wide spectrum of ethnic groups, we demonstrate long-range linkage disequilibrium among CD28, CTLA4, and ICOS costimulatory genes. PMID: 17197413
  80. ICOS may be relevant in inducing an acute immune response and may be critically involved in perpetuating inflammation in chronically immune-mediated disorders of the peripheral nervous system. PMID: 17242332
  81. These results define elevated populations of memory T-lymphocytes expressing B7 molecules in rheumatoid arthritis (RA) synovial fluid that either stimulate T cells through ICOS, or down-regulate RA synovial tissue T-lymphocytes through B7H1 and B7H2. PMID: 17323353
  82. The whole blood gene expression quantities of costimulatory molecules CD154 and ICOS reasonably robustly differentiated rejection patients from control patients. PMID: 17414714
  83. ICOS gene haplotypes correlate with IL10 secretion and multiple sclerosis. PMID: 17481737
  84. definition of a newly discovered pathway that prevents autoimmunity by limiting the levels on T lymphocytes of aco-stimulatory receptor, the inducible T-cell co-stimulator(ICOS) PMID: 18172933
  85. Modulation of immune responses by ICOS-expressing naturally occurring T regulatory cells present in melanoma tissue depends upon costimulatory signals delivered to the CD4-positive CD25-negative responder cells and the cytokine milieu. PMID: 18292519
  86. p50alpha is likely a determining factor in ICOS-mediated PI3K activity in T cells PMID: 18641334
  87. Children with type 1 diabetes show decreased inducrion of ICOS in T cells. PMID: 18973208
  88. The shared CTLA4-ICOS risk locus in celiac disease, IgA deficiency and common variable immunodeficiency. PMID: 19020530
  89. ICOS-induced T cell spreading and filopodia/microspike formation may promote antigen recognition by enhancing a T cell's scanning potential of an adherent APC surface. PMID: 19095735
  90. ICOS variants do not play a major role in the development of atopy in European children. PMID: 19175887
  91. ICOS could enhance the cytotoxic activity of Cytokine-induced killer cells (CIK) cells, in part, by augmenting cytokine secretion and prolonging cell survival both in vitro and in vivo. PMID: 19194830
  92. ICOS plays a crucial role in the induction of peripheral tolerance maintained by tolerogenic dendritic cells. PMID: 19265111
  93. results provide evidence for rheumatoid arthritis and primary biliary cirrhosis association with the CTLA4/ICOS locus and suggest that the risk allele(s) within this region may be common to both diseases PMID: 19333938
  94. study confirms the involvement of the CTLA-4 but not the ICOS gene in susceptibility to type 1 diabetes. PMID: 19376178
  95. These findings confirmed the dysfunction of both CD4(+) and CD8(+) T-cells after ICOS costimulation in lupus patients. Impairment of ERK activation involved in ICOS-mediated IL-2 and T-cell hypoproliferation in active SLE. PMID: 19376499
  96. Extensive T cell dysfunction, decreased memory T cell compartment, and imbalance between effector and regulatory cells in ICOS-deficient patients may underlie their immunodeficiency and/or autoimmunity. PMID: 19380800
  97. mRNA level for CTLA-4, ICOS1, IL-23, IL-27, SMAD3 and GITR were lower in T regulatory cells of children with diabetes compared to the control patients PMID: 19547759

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Involvement in disease Immunodeficiency, common variable, 1 (CVID1)
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Secreted
Tissue Specificity Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral
Database Links

HGNC: 5351

OMIM: 604558

KEGG: hsa:29851

STRING: 9606.ENSP00000319476

UniGene: Hs.56247

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