Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MAPK10 Polyclonal antibody

Uniprot No.

P53779

Target Names

MAPK10

Alternative Names

c Jun kinase 3 antibody; c-Jun N-terminal kinase 3 antibody; cJun N terminal kinase 3 antibody; FLJ12099 antibody; FLJ33785 antibody; JNK3 alpha protein kinase antibody; JNK3 antibody; JNK3A antibody; MAP kinase 10 antibody; MAP kinase antibody; MAP kinase p49 3F12 antibody; MAPK 10 antibody; Mapk10 antibody; MGC50974 antibody; mitogen activated protein kinase 10 antibody; Mitogen-activated protein kinase 10 antibody; MK10_HUMAN antibody; p493F12 antibody; p54bSAPK antibody; PRKM10 antibody; protein kinase mitogen activated 10 antibody; SAPK1b antibody; Stress activated protein kinase 1b antibody; stress activated protein kinase beta antibody; Stress activated protein kinase JNK3 antibody; Stress-activated protein kinase JNK3 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from internal of Human MAPK10.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1.

Gene References into Functions

Mapk10 expression was regulated by miR27a-3p in nasopharyngeal carcinoma.Mapk10 gene was down-regulated in the nasopharyngeal carcinoma cells. PMID: 28393229
Peptide mini-scaffold facilitates JNK3 activation in cells. PMID: 26868142
Study found that JNK3 levels are increased in brain tissue and CSF from patients with Alzheimer disease and CSF levels could reflect the rate of cognitive decline PMID: 25455349
Data indicate that tetra-substituted pyridinylimidazoles were designed as dual inhibitors of c-Jun N-terminal kinase (JNK) 3 and p38alpha mitogen-activated protein (MAP) kinase, and both kinases may be involved in the progression of Huntington's disease. PMID: 25475894
JNK3 is required for the antiapoptotic effects of exendin 4 PMID: 25025079
analysis of the unique mechanisms by which JNK1beta1 is regulated PMID: 25178256
Mitogen-activated protein kinase 10 JNK3 alpha (JNK3apha2)binds to both domains of arrestin-3. PMID: 24412749
miR-29b mRNA, MAPK10 protein expression, and ATG9A protein expression are closely related to chemosensitivity of ovarian carcinoma. PMID: 24767251
Subtle structural mechanisms for allosteric signaling between the peptide-binding site and activation loop of human JNK3. PMID: 23142346
reduced JNK3 activity has potentially deleterious effects on neuronal function via altered regulation of a set of post-synaptic proteins. PMID: 23329067
Silent scaffolds: inhibition OF c-Jun N-terminal kinase 3 activity in cell by dominant-negative arrestin-3 mutant. PMID: 22523077
[review] This review focuses on delineating the role of scaffold proteins, especially that of JNK3 as a target, on the regulation of JNK signaling in neurons. PMID: 21321401
Arrestin-3 acts as a "true" scaffold, facilitating JNK3alpha2 phosphorylation by bringing it and MAP kinase kinase (MKK)4 together. PMID: 22047447
The results suggest the possible involvement of CaMKII and JNK3 in soman-induced long-term neurotoxicity. PMID: 21041242
results suggest that MAPK10 may have a proapoptotic function and could function as a tumor-suppressor gene in chromophobe renal cell carcinoma PMID: 21166945
Shares a promoter region with the tightly linked gene encoding Fas-associated phosphatase-1. PMID: 12436199
interaction of free arrestins with JNK3 and Mdm2 and their ability to regulate subcellular localization of these proteins may play an important role in the survival of photoreceptors and other neurons PMID: 16737965
Arrestin in all conformations binds JNK3 comparably, whereas Mdm2 preferentially binds cone arrestin 'frozen' in the basal state. PMID: 17680991
JNK3 recruits MKK4 to the beta-arrestin-2 scaffold complex by binding to the MAPK docking domain (D-domain) located within the N terminus of MKK4. PMID: 18408005

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Involvement in disease

A chromosomal aberration involving MAPK10 has been found in a single patient with pharmacoresistant epileptic encephalopathy. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.

Subcellular Location

Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion.

Protein Families

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily

Tissue Specificity

Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

Database Links

HGNC: 6872

OMIM: 602897

KEGG: hsa:5602

STRING: 9606.ENSP00000352157

UniGene: Hs.125503

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Code	CSB-PA932566
Size	US$297
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Image	Western blot analysis of extracts from HeLa cells, 293 cells and Jurkat cells, using MAPK10 antibody.
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MAPK10 Antibody

Product Details

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Target Background