Code | CSB-RA240216A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
The generation of the MAPK10 recombinant monoclonal antibody is a carefully executed process, designed to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal, with the a synthesized peptide derived from human MAPK10 used as the immunogen. Subsequently, total RNA is extracted from the isolated B cells and converted into cDNA through reverse transcription. The MAPK10 antibody genes are then amplified using PCR with specific primers that target the antibody constant regions, followed by their insertion into an expression vector. The expression vector is introduced into host cells, allowing for the production of the MAPK10 recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and subjected to affinity chromatography for purification, resulting in a highly purified form. To confirm its specificity and functionality, the antibody undergoes extensive characterization assays, including ELISA, WB, IHC, IF, and FC analysis, ensuring its precise recognition of human and mouse MAPK10 proteins.
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