Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

NLRP3

Uniprot No.

Q96P20

Research Area

Others

Alternative Names

(Angiotensin/vasopressin receptor AII/AVP-like)(Caterpiller protein 1.1)(CLR1.1)(Cold-induced autoinflammatory syndrome 1 protein)(Cryopyrin)(PYRIN-containing APAF1-like protein 1)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

733-1036aa

Target Protein Sequence

LFSVLSTSQSLTELDLSDNSLGDPGMRVLCETLQHPGCNIRRLWLGRCGLSHECCFDISLVLSSNQKLVELDLSDNALGDFGIRLLCVGLKHLLCNLKKLWLVSCCLTSACCQDLASVLSTSHSLTRLYVGENALGDSGVAILCEKAKNPQCNLQKLGLVNSGLTSVCCSALSSVLSTNQNLTHLYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

40.7 kDa

Protein Length

partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant human NLRP3 production in E. coli is a multi-step process. First, co-inserting the gene of interest into an expression vector with an N-terminal 10xHis-tag and C-terminal Myc-tag gene. The target gene encodes the 733-1036aa of human NLRP3. And then, transfecting the recombinant vector into E. coli cells. The cells are cultured to produce the NLRP3 protein, which is extracted by cell lysis. Purification of the collected recombinant human NLRP3 protein is done using affinity chromatography. Its purity is assessed using SDS-PAGE, 85%.

The NLRP3 is a key component of the innate immune system involved in inflammatory responses. It is a multi-domain protein comprising an amino-terminal pyrin domain (PYD), a central NACHT domain, and a carboxy-terminal leucine-rich repeat domain (LRR domain) [1][2]. Activation of NLRP3 results in the assembly of the NLRP3-ASC-pro-caspase-1 inflammasome, which activates caspase-1, leading to the release of proinflammatory cytokines IL-1β and IL-18 in response to various stimuli like microbial infections and cellular damage [3][4][5].

NLRP3 functions as a sensor for a broad range of PAMPs and DAMPs, enabling the detection of cellular stress signals induced by infection and sterile inflammation [6][7]. Aberrant NLRP3 activity can cause excessive inflammation, contributing to the development of various chronic diseases [8].

References:
[1] K. Swanson, M. Deng, & J. Ting, The nlrp3 inflammasome: molecular activation and regulation to therapeutics, Nature Reviews Immunology, vol. 19, no. 8, p. 477-489, 2019. https://doi.org/10.1038/s41577-019-0165-0
[2] I. Hochheiser, M. Pilsl, G. Hagelueken, J. Moecking, M. Marleaux, R. Brinkschulteet al., Structure of the nlrp3 decamer bound to the cytokine release inhibitor crid3, Nature, vol. 604, no. 7904, p. 184-189, 2022. https://doi.org/10.1038/s41586-022-04467-w
[3] X. Shi, S. Tan, & S. Tan, Nlrp3 inflammasome in sepsis (review), Molecular Medicine Reports, vol. 24, no. 1, 2021. https://doi.org/10.3892/mmr.2021.12153
[4] N. Kelley, D. Jeltema, Y. Duan, & Y. He, The nlrp3 inflammasome: an overview of mechanisms of activation and regulation, International Journal of Molecular Sciences, vol. 20, no. 13, p. 3328, 2019. https://doi.org/10.3390/ijms20133328
[5] L. El-Sharkawy, D. Brough, & S. Freeman, Inhibiting the nlrp3 inflammasome, Molecules, vol. 25, no. 23, p. 5533, 2020. https://doi.org/10.3390/molecules25235533
[6] N. Song and T. Li, Regulation of nlrp3 inflammasome by phosphorylation, Frontiers in Immunology, vol. 9, 2018. https://doi.org/10.3389/fimmu.2018.02305
[7] A. Akbal, A. Dernst, M. Lovotti, M. Mangan, R. McManus, & E. Latz, How location and cellular signaling combine to activate the nlrp3 inflammasome, Cellular and Molecular Immunology, vol. 19, no. 11, p. 1201-1214, 2022. https://doi.org/10.1038/s41423-022-00922-w
[8] Z. Zhong, S. Liang, E. Sánchez-López, F. He, S. Shalapour, X. Linet al., New mitochondrial dna synthesis enables nlrp3 inflammasome activation, Nature, vol. 560, no. 7717, p. 198-203, 2018. https://doi.org/10.1038/s41586-018-0372-z

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Microscale thermophoresis (MST) technology-based assay

Review: In the assay of the dye-labelled PPM1A inter_x0002_action with LRR domain of NLRP3 (LRR-NLRP3), a concentration series of LRR-NLRP3 were prepared using a 1:1 serial dilution of PPM1A in PBS with 0.1% Tween 20.

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Target Background

Function

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF.

Gene References into Functions

In conclusion, IAPP/amylin directly interacts with NLRP3 to activate NLRP3 inflammasome, and this interaction could be an attractive drug target to avoid inflammation and beta-cell death during therapy for diabetes, although there are mechanisms in NLRP3 inflammasome and diabetes pathology in human tissues that still require elucidation. PMID: 30014749
this study strongly highlights the importance of renal epithelium in lipid handling and recognizes the role of NLRP3 as a central hub in metainflammation and immunometabolism in parenchymal non-immune cells. PMID: 28588189
S100A12 activates NLPR3 inflammasomes to induce MUC5AC production in airway epithelial cells. ATP induces MUC5AC production in a mechanistically similar mode to S100A12. PMID: 29906464
Our study indicates that the NLRP3 rs4612666 polymorphism may be involved in the development of recurrent aphthous stomatitis in the Czech population PMID: 29430721
sumoylation of NLRP3 restrains inflammasome activation, and identify SUMO proteases as potential drug targets for the treatment of inflammatory diseases. PMID: 30069026
Keratoendotheliitis fugax hereditaria is an autoinflammatory cryopyrin-associated periodic syndrome caused by a missense mutation c.61G>C in exon 1 of NLRP3 in Finnish patients. It is additionally expected to occur in other populations of European descent. PMID: 29366613
Twelve out of 15 Chinese children with cryopyrin-associated periodic syndrome (CAPS) have mutation in NLR Family, Pyrin Domain-Containing 3 Protein (NLRP3). Five new mutation sites on NLRP3 are identified. The association between gene mutation and clinical manifestation shows that D305N is highly associated with severe organs involvements. PMID: 29285715
our results reveal that the overexpressed NLRP3 contributes to the proliferation and metastasis of OSCC cells. PMID: 29716544
NLRP3 inflammasome was activated during the clearance of the aged erythrocytes through unopsonized and opsonized pathways PMID: 29680907
NLR family pyrin domain containing 3 (NLRP3) may be a novel player in testicular immune regulation PMID: 29907661
Study indicates that NLRP3 affects human glioma progression and metastasis through multiple pathways, including epithelial-mesenchymal transition and PTEN/AKT signaling pathway regulation, enhanced inflammasome activation, and undefined inflammasome-independent mechanisms. PMID: 30015880
increased mRNA levels of NLRP3 inflammasomes in bronchial tissues were positively correlated with the load of the six common pathogens in the lower respiratory tract PMID: 29656319
gain-of-function variant rs10754558 significantly associated with obesity PMID: 28634744
Obese antral muscle cells showed an oxidative-inflammatory imbalance with overexpression of NLRP3 inflammasome, increased IL-1beta secretion and caspase1-activation, and reduced antioxidant capacity associated with a myogenic motor impairment of VIP-induced relaxation. PMID: 29625905
The data supports the assumption that C9 gene expression may stimulate the expression of inflammatory (NLRP3) and angiogenic growth factors (VEGF) in retinal pigment epithelial cells. PMID: 30090015
these results identified endothelial cells as a target of heme-mediated NLRP3 activation that can contribute to the inflammation triggered by sterile hemolysis PMID: 29743981
Using keratinocyteinduced HaCaT cells as a model for psoriasis, silencing of miR155 was confirmed to significantly decrease inflammation and NLRP3/caspase1 signaling. PMID: 29767259
Anaplastic lymphoma kinase (ALK) is a novel regulator of NLRP3 inflammasome activation in macrophages. Mechanically, ALK-mediated NF-kappaB activation was required for the priming step of NLRP3 upregulation, whereas ALK-mediated lipid peroxidation contributed to the sensing step of NLRP3-NEK7 complex formation. PMID: 29723525
this review presents the current knowledge regarding the function of NLRP3 in vascular disease, ischemic heart disease, and nonischemic heart disease PMID: 29850631
sing in vitro and in vivo models, we found that canonical NLRP3 and non-canonical caspase-11 inflammasomes were activated in bone-associated MSCs (BA-MSCs) to promote the inflammatory response PMID: 29807832
The glucometabolic state is of great importance on both the circulating level of IL-18 and the genetic expression of IL-18 and NLRP3 in adipose tissue. PMID: 29972041
eight single nucleotide polymorphisms, four from NLRP1 (rs8079034, rs11651270, rs11657747, and rs878329) and NLRP3 (rs7512998, rs3806265, rs10754557, and rs10733113) each in 540 patients with Psoriasis Vulgaris and 612 healthy controls in the Chinese Han population, were genotyped. PMID: 29850521
Studied association between nucleotide-binding oligomerization domain leucine rich repeat and pyrin domain containing 3 (NLRP3) gene polymorphisms and susceptibility for large artery atherosclerosis (LAA), ischemic stroke (IS) and microembolic signals (MES) in the Chinese Han population. PMID: 29850543
Data indicate that by inhibiting adenine nucleotide translocase 1 (ANT1) and mitochondrial dysfunction, tyrosine phosphatase SHP2 orchestrates an intrinsic regulatory loop to limit excessive NLR family, pyrin domain-containing 3 protein (NLRP3) inflammasome activation. PMID: 29255148
our results indicate that NLRP3 inflammasome is a promising target for blocking or retarding EMT-mediated fibrosis in pulmonary silicosis. On basis of this mechanism, pirfenidone might be a potential drug for the treatment of silicosis PMID: 29258746
This study demonstrated that Hcy activates adipose NLRP3 inflammasomes in an adipocyte lyso-PC-dependent manner and highlights the importance of the adipocyte NLRP3 inflammasome in insulin resistance. PMID: 29735414
this study identifies a new role of ILF2 in the regulation of the NLRP3 inflammasome. PMID: 29655789
SeV V protein inhibited the assembly of NLRP3 inflammasomes, including NLRP3-dependent ASC oligomerization, NLRP3-ASC association, NLRP3 self-oligomerization, and intermolecular interactions between NLRP3 molecules. PMID: 30021903
this study shows that NLRP3 regulates inflammation-induced pro-labor mediators in human myometrial cells PMID: 29430761
The increased expression of NLRP3 and caspase-1 in fetal membrane and placental tissues may be associated with the development of premature rupture of membrane. PMID: 29545514
Oxidative stress activates NLRP3 through upregulating CTSB activity. PMID: 29196167
Suggest inflammasome protein NLRP3 appears to have a specific role in the development of abdominal aortic aneurysms and aortic occlusive disease. PMID: 29528779
Our results showed that rs10754558 NLRP3 and rs2043211 CARD8 polymorphisms are associated with rheumatoid arthritis development (p value = 0.044, OR = 1.77, statistical power = 0.999) and severity measured by Health Assessment Questionnaire (HAQ) (p value = 0.03), respectively. PMID: 29230505
Based on the data obtained from patients and in vitro cells, we concluded that both NLRP1 and NLRP3 inflammasomes are highly involved in the FLS inflammation and pyroptosis. PMID: 29393464
miR-132 negatively regulates palmitate induced NLRP3 inflammasome activation through FOXO3 down-regulation in THP-1 cells. PMID: 29258239
25-hydroxycholesterol contributes to cerebral inflammation of X-linked adrenoleukodystrophy through activation of the NLRP3 inflammasome. PMID: 27779191
NLRP3 inflammasomes are upregulated in adults with diabetic retinopathy (DR) and may play a key role in the pathogenesis and progression of DR. PMID: 29450537
The GG genotype of rs10754558 and the CGA haplotype of rs4612666-C, rs10754558-G, and rs1539019-A are both independent risk factors for primary gouty arthritis development. The rs10754558 polymorphism might participate in regulating immune and inflammation responses in patients with gouty arthritis by influencing the expression of components of the NLRP3 inflammasome. PMID: 29214547
Data show that Pr2x7 gene deletion protects from HFD-induced NASH, possibly through blunted activation of NLRP3 inflammasome. PMID: 29270247
This study revealed that the P2X7R/NLRP3 pathway plays important roles in IL-1beta secretion and inhibition of Toxoplasma gondii proliferation in small intestinal epithelial cells. PMID: 29291748
Therefore, the common allergen Der f1 was not only found to induce allergy, but also led to pyroptosis and IL1beta secretion via the NLRP3caspase1 inflammasome in human bronchial epithelial cells PMID: 29207030
NLRP3 mRNA expression was decreased significantly in chronic myeloid leukemia patients PMID: 29097263
miR-223 deficiency can lead to the sustained activation of NLRP3-IL-1beta. PMID: 29144508
Review. NLR Family, Pyrin Domain-Containing 3 Protein (NLRP3)inflammasome activation upon sensing influenza is essential for host defense but can increase disease. Virally produced influenza proteins can either inhibit or enhance NLRP3 inflammasome activation. Inhibition of the NLRP3 inflammasome to reduce influenza disease must be critically timed to be of benefit. PMID: 29097227
Data show that in HK-2 cells and unilateral nephrectomy model, NLRP3 and NLRP3 inflammasome member ASC were significantly augmented after treatment with contrast media and provide evidence that NLRP3 inflammasome mediated contrast-induced-acute kidney injury through modulating the apoptotic pathway. PMID: 27721494
BCL6 overexpression in SHR reduced blood pressure, NLRP3 expression and inflammation in the renal cortex of SHR PMID: 29072703
we have shown that a mutation of the NLRP3 gene can cause hearing loss in the absence of the other clinical signs and symptoms of CAPS that are associated with other mutations of NLRP3. we show that a missense mutation, p.Arg918Gln (c.2753G > A), of NLRP3 causes autosomal-dominant sensorineural hearing loss in two unrelated families PMID: 28847925
Results demonstrated that in patients with ulcerative colitis (UC), expression of the NLRP3 protein was markedly enhanced in the inflamed colonic mucosa; expression levels of NLRP3 and its colocalization with cleaved caspase-1 positively correlated with disease severity. Also, further data suggested that the NLRP3 inflammasome might play a protective role in the pathogenesis of UC. PMID: 27966619
P2X7 receptor inhibition attenuated sympathetic nerve sprouting after myocardial infarction via the NLRP3/IL1B pathway. PMID: 28470940
Review/Meta-analysis: NLRP3 p.Q705K snp not associated with the susceptibility to rheumatoid arthritis. PMID: 28185410

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Involvement in disease

Familial cold autoinflammatory syndrome 1 (FCAS1); Muckle-Wells syndrome (MWS); Chronic infantile neurologic cutaneous and articular syndrome (CINCA)

Subcellular Location

Cytoplasm, cytosol. Inflammasome. Endoplasmic reticulum. Secreted. Nucleus.; Golgi apparatus membrane.

Protein Families

NLRP family

Tissue Specificity

Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level). Expression in monocytes is very weak (at protein level). Expr

Database Links

HGNC: 16400

OMIM: 120100

KEGG: hsa:114548

STRING: 9606.ENSP00000337383

UniGene: Hs.159483

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Code	CSB-EP822275HU2
MSDS	MSDS
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human NACHT, LRR and PYD domains-containing protein 3 (NLRP3), partial

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