Recombinant Human Serum amyloid P-component(APCS)

Code CSB-EP001898HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names APCS
Uniprot No. P02743
Research Area Neuroscience
Alternative Names 9.5S alpha 1 glycoprotein; 9.5S alpha-1-glycoprotein; Amyloid P component serum; APCS; MGC88159; Pentaxin related; Pentraxin 2; PTX 2; PTX2; SAMP_HUMAN; SAP; Serum Amyloid P; Serum amyloid P component; Serum amyloid P-component(1-203)
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-223aa
Target Protein Sequence HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 50.3kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin.
Gene References into Functions
  1. The findings suggest that the F57I mutation affects the aggregation process of lysozyme resulting in the formation of cytotoxic species and that SAP is able to prevent cell death in the F57I flies by preventing accumulation of toxic F57I structures. PMID: 27428539
  2. Data suggest that SAP (serum amyloid P-component) interacts with at least 33 proteins/ligands in plasma containing physiological calcium levels; these ligands can be categorized to include apolipoproteins, complement system proteins, coagulation proteins, and components that regulate proteolysis. PMID: 28098450
  3. Data suggest that serum haptoglobin, fetuin-A, platelet factor-4, hs-CRP (high sensitive-C-reactive protein), SAP (serum amyloid P), AGP (alpha1-acid glycoprotein) levels of adolescents with metabolic syndrome are significantly higher than those of controls subjects. PMID: 27754964
  4. SNPs in APCS were associated with late age at onset in familial amyloid polyneuropathy. PMID: 26286643
  5. Data suggest that serum amyloid P (SAP) activates CD209 DC-SIGN to regulate the innate immune system differently from C-reactive protein (CRP), and that DC-SIGN is a target for antifibrotics. PMID: 26106150
  6. Here, it is reported that X-ray analysis of the SAP complex with CPHPC and cadmium ions provides higher resolution detail of the interaction than is observed with calcium ions. PMID: 25084341
  7. Compared to wildtype C57BL/6 mice, we find that in Apcs-/- "SAP knock-out" mice, bleomycin induces a more persistent inflammatory response and increased fibrosis. PMID: 24695531
  8. The effects of SAP and Xiapex (Collagenase Clostridium histolyticum) on fibrocytes derived from Dupuytren's disease, was evaluated. PMID: 24933153
  9. plasma protein serum amyloid P binds to FcgammaRI on monocytes to inhibit fibrocyte differentiation, and binds to FcgammaRIIa on neutrophils to reduce neutrophil adhesion. PMID: 25024390
  10. In the late phase post-myocardial infarct there is a coordinated decrease in immune response-inflammation proteins, except for SAP which showed an increase related to the specific activation of the classical complement pathway. PMID: 23992930
  11. 9 different proteins (haptoglobin, transthyretin, apolipoprotein A-1, serum amyloid P component, apolipoprotein E, complement factor H, fibrinogen gamma, thrombin, complement C3) were identified as a potential diagnostic pattern of Parkinson's disease. PMID: 23385359
  12. Human SAP binds phosphoethanolamine, while rabbits seem to have low levels of SAP that bind to a lesser extent. PMID: 23600950
  13. These results indicate that SAP functions as a host defense factor, similar to other peptidoglycan recognition proteins and nucleotide-binding oligomerization domain-like receptors. PMID: 23966633
  14. SAP may act as an effective receptor mimic to limit influenza A virus infection of airway epithelial cells. PMID: 23544079
  15. Persons who develop non-affective psychoses have lower levels of certain acute phase proteins, including SAP, at the time of birth. PMID: 23423137
  16. [review] On the basis of structure, serum amyloid P component is a prototype of the short pentraxin family. PMID: 23527487
  17. SAP has a new role in reducing the toxicity of early amyloidogenic aggregates in transthyretin amiloidosis. PMID: 23390551
  18. observations suggest that serum amyloid P, at least in part, uses FcgammaRI and FcRgamma to inhibit fibrocyte differentiation PMID: 22493081
  19. Low levels of serum amyloid P mark the brains of individuals who escape dementia despite the presence of beta amyloid plaques and tangles in Alzheimer's disease neuropathology. PMID: 22205573
  20. Studies indicate that pharmaceutical grade serum amyloid P component and C-reactive protein were isolated and purified. PMID: 22867744
  21. The START domain in GPBP is important for this interaction. SAP and GPBP form complexes in blood and partly colocalize in amyloid plaques from Alzheimer disease patients. PMID: 22396542
  22. Serum amyloid P level increased by approximately 5-fold in Parkinson's disease samples;a potential feasibility of plasma amyloid P as a marker to approach Parkinson's disease PMID: 21223953
  23. Under physiological conditions, phosphoethanolamine is bound with higher affinity by human SAP than by human CRP. PMID: 21360619
  24. TGF-beta driven lung fibrosis is macrophage dependent and blocked by Serum amyloid P. PMID: 21044893
  25. these data suggest that local production of serum amyloid P and c-reactive protein in the alzheimer disease brain does not substantially contribute to the CSF levels. PMID: 20930309
  26. Human SAP inhibits DNA-mediated innate immune activation in vitro and may limit innate and adaptive immune responses after DNA vaccination of transgenic mice. PMID: 21278351
  27. serum SAP levels may be an easy detected predictor for the healing of burn wounds PMID: 20932823
  28. Interaction between MBL and PTX3 led to communication between the lectin and classical complement pathways via recruitment of C1q, whereas SAP-enhanced complement activation occurs via a hitherto unknown mechanism PMID: 21106539
  29. SAP has an effect on macrophages in fibrotic lung disease PMID: 20300636
  30. Functional analysis demonstrated that SAP associated with HDL promotes SR-BI-dependent cholesterol efflux and lipid-free SAP enhances ABCA1-dependent cholesterol efflux PMID: 20189569
  31. A possible role of SAP in either host resistance or viral virulence was investigated during influenza infection in vivo. Influenza virus infection is not affected by serum amyloid P component.Human SAP binds much more avidly than mouse SAP to the virus. PMID: 11984001
  32. Targeted pharmacological depletion of serum amyloid P component for treatment of human amyloidosis. PMID: 12015594
  33. serum amyloid P component does not circulate in complex with C4-binding protein, fibronectin or any other major protein ligand PMID: 12100475
  34. structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose PMID: 12126626
  35. Serum amyloid p component binds to late apoptotic cells and is involved in the phagocytosis of these cells by human monocyte-derived macrophages. PMID: 12528126
  36. Serum amyloid P component binding to Shiga toxin 2 requires both a subunit and B pentamer. PMID: 14500533
  37. Purified SAP inhibits fibrocyte differentiation at levels similar to those found in plasma, while depleting SAP reduces the ability of plasma to inhibit fibrocyte differentiation. PMID: 14607961
  38. This protein, a non-fibrillar component, causes soluble fibrils to condense into localized fibrillar aggregates with a greatly enhanced local density of fibril entanglements. PMID: 15031287
  39. Genetic variation in APCS is associated with amyloid polyneuropathy PMID: 15649951
  40. Amyloid-associated SAP significantly increases fibrillar prion protein-related peptide-induced release of interleukin-6 and TNF-alpha from human microglia. PMID: 15837583
  41. Review discusses clinical significance of different levels of SAP, its role in induction or protection from autoimmunity, and the presence of specific SAP autoantibodies in different autoimmune diseases. PMID: 16380821
  42. the shared specificity as well as their shared capability to activate complement, suggest that IgM and the pentraxins CRP and SAP exert similar functions in the removal of apoptotic cells PMID: 16643876
  43. Monocytes bound biotinylated serum amyloid P component with avidity in a dose-dependent and saturable manner; speculated that binding of SAP by monocytes could be of physiological relevance at extravascular sites by influencing complement regulation PMID: 16784490
  44. Serum amyloid P (SAP) and C-reactive protein (CRP) may represent different facets of inflammation. The association of SAP with cardiovascular disease in these older adults further supports the role of innate immunity in atherosclerosis. PMID: 17138933
  45. No reduced circulating concentrations of serum amyloid P component (SAP) in patients with systemic sclerosis, nor any evidence of an association between SAP levels and the extent or severity of fibrosis were observed. PMID: 17530641
  46. SAP may modulate the inflammatory response to amyloid fibrils in atherosclerosis PMID: 17630380
  47. SAP was quantitated using PVDF affinity probes and MALDI-MS. PMID: 17676666
  48. The complex structure between human SAP and FcgammaRIIa reveals a diagonally bound receptor on each SAP pentamer with both D1 and D2 domains of the receptor contacting the ridge helices from two SAP subunits. PMID: 19011614
  49. Our data suggest that measurement of cerebrospinal fluid SAP levels can aid in the identification of incipient Alzheimer's disease among mild cognitive impairment patients PMID: 19052452
  50. Serum amyloid P binds to cells in the early stage of apoptosis, presumably via phosphatidylethanolamine exposed in flip-flopped membranes, suggesting a role for serum amyloid P in the clearance of these cells in vivo. PMID: 11441067

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Involvement in disease SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
Subcellular Location Secreted
Protein Families Pentraxin family
Tissue Specificity Found in serum and urine.
Database Links

HGNC: 584

OMIM: 104770

KEGG: hsa:325

STRING: 9606.ENSP00000255040

UniGene: Hs.507080

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