Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Vim

Uniprot No.

P20152

Research Area

Cancer

Alternative Names

VimVimentin

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-466aa

Target Protein Sequence

STRSVSSSSYRRMFGGSGTSSRPSSNRSYVTTSTRTYSLGSALRPSTSRSLYSSSPGGAYVTRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPTFSSLNLRETNLESLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

61.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Just like other recombinant proteins, the production of this recombinant Mouse Vim protein began with appropriate cDNA and PCR methods, and then the Vim expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Mouse Vim protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 85%+.

Vim is a gene encoding a protein named vimentin in mouse. Other specieses, such as human, also have vimentin protein. It has been reported that vimentin, a cytoskeleton filament that is expressed only in mesenchymal cells after birth. It is re-expressed in epithelial cells in vivo under pathological conditions and in vitro in primary culture. This protein is involved in many biological processes, such as astrocyte development, regulation of mRNA stability, regulation of mRNA stability, etc. Recombinant vim A can be used to researching the interaction of proteins, such as plectin and vim.

Target Background

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene References into Functions

The Listeria monocytogenes virulence factor InlF was found to bind vimentin and was necessary for optimal bacterial colonization of the brain. PMID: 29487235
MAGE-G1 interacted with fascin 1 or vimentin in P19 cells after a 6-day retinoic acid-induced neuronal differentiation. PMID: 28374796
Results found that the absence of vimentin impairs spontaneous endothelial differentiation in vitro and have furthering the understanding of the regulators of differentiation. PMID: 27480130
Protein phosphatase 1 is a key protein serine/threonine phosphatase that controls vimentin Ser-56 dephosphorylation in smooth muscle. PMID: 27457922
These findings identify vimentin as a positive regulator of stemness in the developing mouse mammary gland and in breast cancer cells. PMID: 28947532
This study is the first to show that vimentin has an important role in tumor metastasis in vivo in the setting of pre-diabetes and endogenous hyperinsulinemia. PMID: 27568979
These findings identify two specific sites on vimentin that are phosphorylated by Cadmium. PMID: 28450285
The expression level of vimentinin liver cirrhotic tissues were significantly higher than that in chronic hepatitis tissues. PMID: 27538444
both arthritis-susceptible and -resistant mice can generate cellular and humoral immunity to Vim. PMID: 27602574
vimentin knockout neurons were insensitive to the axonotrophic effects of Clostridium botulinum C3 exoenzyme PMID: 27419376
These findings suggest that Plk1 regulates smooth muscle contraction by modulating vimentin phosphorylation at Ser-56. PMID: 27662907
findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition PMID: 26251181
these findings identify a hereto-unappreciated role for serine-38 phosphorylated vimentin as an important determinant of myofibroblast sensitivity to Withaferin A. PMID: 26186445
Vimentin expression increased after traumatic brain injury and was positively correlated with edema and neurological impairments. PMID: 26039099
Annexin, lamin, and vimentin were identified as universal dystrophic markers PMID: 26102067
Astrocytes deficient of GFAP and vimentin showed decreased Notch signal sending competence and altered expression of Notch signaling pathway-related genes PMID: 26118771
Absence of GFAP, or both GFAP and vimentin, alters Alzheimer's disease-induced changes in gene expression profile of astrocytes, showing a compensation of the decrease of neuronal support genes and a trend for a higher inflammatory expression profile PMID: 25731615
Vimentin is not only a major organizing element of the intermediate filament network but is also involved in both binding and uptake of C3 exoenzyme. PMID: 24967582
Cytokinetic Failure-induced Tetraploidy Develops into Aneuploidy, Triggering Skin Aging in Phosphovimentin-deficient Mice. PMID: 25847236
Vimentin has a role in regulating activation of the NLRP3 inflammasome PMID: 25762200
protein(s) that associated with RPTPbeta in response to IGF-I and IGFBP-2 in vascular smooth muscle cells PMID: 25787077
Data suggest MAP kinase-interacting kinases (Mnk1, Mnk2) regulate cell migration/wound healing, expression of Vim, stability of Vim, and binding of eIF4E (eukaryotic translation initiation factor 4E)/Cyfip1 (cytoplasmic FMR1 interacting protein 1). PMID: 25588502
This review discusses various novel functions which are now known to be mediated by vimentin, summarizing structure, regulation and roles of vimentin in cell adhesion, migration, angiogenesis, neurite extension, and cancer. PMID: 24387004
Double-immunostaining experiments with antibodies against Stk33 and vimentin showed a striking colocalization of Stk33 and vimentin in the hypothalamus. PMID: 24057876
Keratocyte activation and differentiation play a key role in fibrosis, and vimentin, a major structural type III intermediate filament, is a required component of this process. PMID: 24854859
absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics PMID: 24223940
We demonstrate, using long-term 4D imaging, that the vimentin intermediate filament establishes mitotic polarity in mammalian cell lines and mediates the asymmetric partitioning of damaged proteins. PMID: 24843142
defect of mitotic vimentin phosphorylation causes microophthalmia and cataract PMID: 24142690
Vimentin synthesis initiates during a differentiation process of trophoblast giant cells and continues throughout the stage of vascular TGC. PMID: 23664004
These data suggest that vimentin is required for the minute virus of mice life cycle, presenting possibly a dual role: (1) following virus escape from endosomes and (2) during endosomal trafficking PMID: 23838001
Data indicate interaction of PPARgamma with vimentin in the cytosolic compartment, in which vimentin regulating the turnover rate of PPARgamma, which further regulate genomic or non-genomic activities through the regulation of PPARgamma protein degradation. PMID: 23297177
involvement of vimentin in migration processes at different stages of development and try to resolve current contradictions concerning the role of vimentin in various events of cell migration PMID: 23885566
we propose a novel regulatory mechanism of IP3R1 activity by type III intermediate filament vimentin PMID: 22929228
Data indicate that vimentin intermediate filaments must coalesce at mature focal adhesions for efficient endoplasmic spreading. PMID: 23115305
These data show for the first time that decorin has an impact on the biology of alpha2beta1 integrin and the vimentin intermediate filament system. PMID: 23226541
overexpression of mesangial integrin alpha1 and podocyte vimentin and integrin alpha3 may be important features of glomerular Alport disease PMID: 23236390
expression detected in the mylohyoid muscle and surrounding tissues at embryonic day 12 PMID: 22476899
TGFbeta stimulates vimentin production via PI3K-Akt-mTOR signaling, which leads to suppression of ATF4-dependent Ocn transcription and osteoblast differentiation. PMID: 22952236
vimentin ablation results in defective steroidogenesis PMID: 22535769
vimentin is a regulator of NRG1 type III function and peripheral nerve myelination PMID: 22357929
Endothelial cell surface vimentin binding peptide induces angiogenesis under hypoxic/ischemic conditions. PMID: 21803052
absence of Zmpste24 profoundly alters the processing of the cytoskeletal protein vimentin PMID: 21828285
Corneal antifibrotic switch identified in genetic and pharmacological deficiency of vimentin. PMID: 22117063
Data demonstrated that Abeta toxic species cross the plasma membrane, accumulate in cells and bind to a variety of internal proteins, vimentin (cytoskeleton), cathepsin D (lysosomes), GRP-78 (endoplasmic reticulum and associated membranes). PMID: 21966382
study reports that vimentin filaments associate with collagen mRNAs in a 5'stem-loop sequence and LARP6-dependent manner and stabilize collagen mRNAs PMID: 21746880
When vimentin organization is disrupted by a dominant-negative mutant or by silencing, there is a loss of polarity. These findings demonstrate an antagonistic relationship between vimentin intermediate filaments (VIF) and the formation of lamellipodia. PMID: 21346197
Mature focal adhesions and their derivative fibronectin fibril-aligned fibrillar adhesions (FbAs) serve as docking sites for vimentin intermediate filaments (IFs) in a plectin isoform 1f (P1f)-dependent manner. PMID: 20702585
Increases in post-traumatic vimentin mRNA levels in the cortex and in the hippocampus appear together with vimentin immunoreactivity in astrocytes starting one day after severe trauma. PMID: 20479526
The interaction of hormone-sensitive lipase with vimentin, and its hormonal dependence, was confirmed by coimmunoprecipitation. PMID: 20143880
Sol-gel polymerization of tetraethoxysilane proceeded preferentially on the surface of intermediate filaments assembled from vimentin protein in vitro, resulting in silica-coated fibres. PMID: 19656809

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

Protein Families

Intermediate filament family

Tissue Specificity

Detected in eye lens fiber cells (at protein level). Expressed in retinal lens epithelial cells (at protein level).

Database Links

KEGG: mmu:22352

STRING: 10090.ENSMUSP00000028062

UniGene: Mm.268000

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Code	CSB-EP025857MO
MSDS	MSDS
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Vimentin (Vim)

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