Code | CSB-RA177985A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
To develop the CDC42 recombinant monoclonal antibody, several steps are involved. The process begins with the harvesting of the CDC42 monoclonal antibody, followed by genetic sequencing of its code. A vector for the CDC42 monoclonal antibody gene is then created and transfected into a host cell line for culture. A synthesized peptide derived from human CDC42 is used to stimulate the CDC42 monoclonal antibody production. The resulting CDC42 recombinant monoclonal antibody is purified using affinity chromatography to ensure high purity. Finally, ELISA, WB, and IHC assays are carried out to validate the antibody's ability to recognize CDC42. It reacts with human and mouse CDC42 proteins.
The CDC42 plays critical roles in regulating cytoskeletal organization, cell polarity, signal transduction, and vesicle trafficking. CDC42 is involved in the regulation of actin cytoskeletal dynamics, which are essential for cell shape changes, cell movement, and cell division. CDC42 activation leads to the formation of filopodia and lamellipodia, which are important for cell migration. CDC42 is involved in the establishment and maintenance of cell polarity, which is critical for various cellular processes, including cell migration, differentiation, and tissue organization. Dysregulation of CDC42 activity has been implicated in various diseases, including cancer, developmental disorders, and neurodegenerative diseases.
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