Code | CSB-RA981267A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
GRB2 is a positive regulator of Ras signaling downstream of many growth factor receptors. It is constitutively expressed in T cells and associates with LAT or the CD3 complex via Shc upon TCR stimulation. In addition to the involvement in basic cellular events such as cell growth, cell proliferation, and metabolism, GRB2 plays an important function in embryogenesis and malignant transformation. GRB2 signaling is critical for cell cycle progression and actin-based cell motility, and consequently, more complex processes such as epithelial morphogenesis, angiogenesis, and vasculogenesis.
The production of the recombinant GRB2 antibody includes extracting RNA from spleen cells that are derived from immunized animals, reversely transcribing the RNA into DNA, sequencing and screening antibody genes, amplifying the heavy chain and light chain genes of the antibody using PCR technology, linking and cloning the genes into a plasma vector, and introducing the vector clone into a mammalian cell for functional antibody expression. The recombinant GRB2 antibody was purified using Affinity-chromatography. It can be used to detect the GRB2 antibody from Human, Mouse, Rat in the ELISA, WB, IHC.There are currently no reviews for this product.