|Have Questions?||Leave a Message or Start an on-line Chat|
CUSABIO got the DNA sequence of the pY15-CDK2 monoclonal antibody that was produced from the splenocytes generated by the human CDK2 synthesized phosphopeptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the phospho-CDK2 (Y15) recombinant monoclonal antibody. It is a rabbit IgG antibody purified using the affinity-chromatography method. This anti-pY15-CDK2 antibody is recommended for ELISA WB, IHC, and IP applications and detects the human CDK2 phosphorylated at Tyr 15 residue.
CDK2, a small serine/threonine kinase, regulates the initiation and progression of the S phase of the cell cycle, and the regulation of CDK2 involves cyclin binding and phosphorylation. Several mechanisms, including phosphorylation and dephosphorylation processes, regulate CDK2 activity. Cables increases Wee1-mediated CDK2 tyrosine 15 phosphorylation, thus decreasing CDK2 kinase activity and inhibiting cell growth. CDK2 is inactivated by phosphorylation of T14 and Y15, and activation of CDK2 needs dephosphorylation of both T14 and Y15 by Cdc25, as well as phosphorylation of T160 by CDK activating kinase (CAK).
Cdc2 related protein kinase antibody; cdc2-related protein kinase antibody; CDC28 antibody; CDC2A antibody; Cdk 2 antibody; CDK1 antibody; CDK2 antibody; CDK2_HUMAN antibody; CDKN2 antibody; Cell devision kinase 2 antibody; Cell division protein kinase 2 antibody; Cyclin dependent kinase 2 antibody; cyclin dependent kinase 2-alpha antibody; Cyclin-dependent kinase 2 antibody; kinase Cdc2 antibody; MPF antibody; p33 protein kinase antibody; p33(CDK2) antibody
|Immunogen||A synthesized peptide derived from Human Phospho-CDK2 (Y15)|
|Immunogen Species||Homo sapiens (Human)|
|Concentration||It differs from different batches. Please contact us to confirm it.|
|Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Tested Applications||ELISA, WB, IHC, IP|
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
|Troubleshooting and FAQs||Antibody FAQs|
|Storage||Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.|
|Lead Time||Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.|
There are currently no reviews for this product.
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1. Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
|Gene References into Functions||
|Subcellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).|
|Protein Families||Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily|