Code | CSB-RA005061A15phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
CUSABIO got the DNA sequence of the pY15-CDK2 monoclonal antibody that was produced from the splenocytes generated by the human CDK2 synthesized phosphopeptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the phospho-CDK2 (Y15) recombinant monoclonal antibody. It is a rabbit IgG antibody purified using the affinity-chromatography method. This anti-pY15-CDK2 antibody is recommended for ELISA WB, IHC, and IP applications and detects the human CDK2 phosphorylated at Tyr 15 residue.
CDK2, a small serine/threonine kinase, regulates the initiation and progression of the S phase of the cell cycle, and the regulation of CDK2 involves cyclin binding and phosphorylation. Several mechanisms, including phosphorylation and dephosphorylation processes, regulate CDK2 activity. Cables increases Wee1-mediated CDK2 tyrosine 15 phosphorylation, thus decreasing CDK2 kinase activity and inhibiting cell growth. CDK2 is inactivated by phosphorylation of T14 and Y15, and activation of CDK2 needs dephosphorylation of both T14 and Y15 by Cdc25, as well as phosphorylation of T160 by CDK activating kinase (CAK).
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