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The ATM recombinant monoclonal antibody is developed using DNA recombinant technology and in vitro genetic manipulation. Initially, animals are immunized with a synthesized peptide derived from human ATM, which induces an immune reaction and facilitates the isolation of B cells. Through screening and selection, B cells with the desired specificity are identified. The genes encoding the light and heavy chains of the ATM antibody are amplified via PCR and inserted into a plasmid vector, which is then transfected into host cells for antibody expression. The ATM recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. With a validated high affinity and specificity for human ATM protein, this antibody is well-suited for ELISA, WB, and IP applications.
The ATM protein is a serine/threonine kinase that plays a crucial role in the cellular response to DNA damage. Its main function is to recognize and initiate the repair of double-strand breaks in DNA, which can be caused by exposure to ionizing radiation or other genotoxic agents. ATM also helps to prevent the replication of damaged DNA by triggering cell cycle checkpoints that allow time for repair or, if the damage is too severe, induce apoptosis. In addition to its role in DNA repair, ATM is also involved in other cellular processes, such as telomere maintenance, oxidative stress response, and regulation of gene expression. Mutations in the ATM gene can lead to the development of ataxia-telangiectasia.
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