The CYBB gene encodes the β-subunit of cytochrome b-245 (commonly known as gp91phox), a critical component of the phagocyte NADPH oxidase complex. This transmembrane protein, also designated NOX2, facilitates superoxide generation by transferring electrons from cytosolic NADPH to molecular oxygen during the "respiratory burst" in innate immunity. Functionally, it partners with p22phox to form flavocytochrome b558, which interacts with regulatory subunits (p47phox, p67phox, p40phox, and Rac) upon phagocyte activation. CYBB-mediated reactive oxygen species (ROS) production is essential for microbial killing through the NOX2/ROS signaling axis, intersecting with inflammatory pathways like NF-κB and NLRP3 inflammasome activation. Mutations in CYBB cause X-linked chronic granulomatous disease (X-CGD), characterized by recurrent bacterial/fungal infections and granuloma formation. Current therapeutic strategies include interferon-γ immunotherapy and hematopoietic stem cell transplantation, with emerging lentiviral gene therapy trials (NCT01855685, NCT02757911) demonstrating preliminary efficacy in restoring NADPH oxidase function.
Recombinant Human Cytochrome b-245 heavy chain (CYBB), partial (CSB-EP006325HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CYBB Recombinant Monoclonal Antibody (CSB-RA006325MA1HU)
Validated Data
Immunofluorescence staining of hela cell with CSB-RA006325MA1HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The following CYBB reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CYBB Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006325KA01HU | CYBB Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA006325LA01HU | CYBB Antibody | Human | ELISA, IHC, IF |
CSB-PA006325LC01HU | CYBB Antibody, FITC conjugated | Human | |
CSB-PA002737 | CYBB Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA005619 | CYBB Antibody | Human | WB, ELISA |
CSB-PA176464 | CYBB Antibody | Human,Mouse | ELISA,IHC |
CSB-PA140004 | CYBB Antibody | Human,Mouse | ELISA,IHC |
CSB-RA006325MA1HU | CYBB Recombinant Monoclonal Antibody | Human | ELISA, IF |
CYBB Antibodies for Shigella flexneri
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006306XA01SZB | cybB Antibody | Shigella flexneri | ELISA, WB (ensure identification of antigen) |
CYBB Antibodies for Escherichia coli (strain K12)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA006306XA01ENV | cybB Antibody | Escherichia coli (strain K12) | ELISA, WB (ensure identification of antigen) |
CYBB Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-EP006325HU1 | Recombinant Human Cytochrome b-245 heavy chain (CYBB), partial | E.coli |
CSB-YP006325HU1 | RecombinantHumanCytochromeb-245heavychain (CYBB), partial | Yeast |
CSB-CF006325HU | Recombinant Human Cytochrome b-245 heavy chain (CYBB) | in vitro E.coli expression system |
CSB-BP006325HU1 CSB-MP006325HU1 CSB-EP006325HU1-B |
Recombinant human Cytochrome b-245 heavy chain, partial | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP006325HU1a2 | Recombinant Human Cytochrome b-245 heavy chain (CYBB), partial | E.coli |
CSB-YP006325HU CSB-EP006325HU CSB-BP006325HU CSB-MP006325HU CSB-EP006325HU-B |
Recombinant Human Cytochrome b-245 heavy chain (CYBB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Yersinia pestis
Code | Product Name | Source |
---|---|---|
CSB-CF893085YAS | Recombinant Probable cytochrome b561 (cybB) | in vitro E.coli expression system |
CSB-YP893085YAS1 CSB-EP893085YAS1 CSB-BP893085YAS1 CSB-MP893085YAS1 CSB-EP893085YAS1-B |
Recombinant Probable cytochrome b561 (cybB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Escherichia coli (strain K12)
Code | Product Name | Source |
---|---|---|
CSB-CF006306ENV | Recombinant Escherichia coli Cytochrome b561 (cybB) | in vitro E.coli expression system |
CSB-YP006306ENV1 CSB-EP006306ENV1 CSB-BP006306ENV1 CSB-MP006306ENV1 CSB-EP006306ENV1-B |
Recombinant Escherichia coli Cytochrome b561 (cybB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Shigella flexneri
Code | Product Name | Source |
---|---|---|
CSB-CF006306SZB | Recombinant Cytochrome b561 (cybB) | in vitro E.coli expression system |
CSB-YP006306SZB1 CSB-EP006306SZB1 CSB-BP006306SZB1 CSB-MP006306SZB1 CSB-EP006306SZB1-B |
Recombinant Cytochrome b561 (cybB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-CF006325PI | Recombinant Pig Cytochrome b-245 heavy chain (CYBB) | in vitro E.coli expression system |
CSB-YP006325PI1 CSB-EP006325PI1 CSB-BP006325PI1 CSB-MP006325PI1 CSB-EP006325PI1-B |
Recombinant Pig Cytochrome b-245 heavy chain (CYBB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF733771MO | Recombinant Mouse Cytochrome b-245 heavy chain (Cybb) | in vitro E.coli expression system |
CSB-YP733771MO1 CSB-EP733771MO1 CSB-BP733771MO1 CSB-MP733771MO1 CSB-EP733771MO1-B |
Recombinant Mouse Cytochrome b-245 heavy chain (Cybb), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Bison bison (American bison) (Bos bison)
Code | Product Name | Source |
---|---|---|
CSB-CF836099BTE | Recombinant Bison bison Cytochrome b-245 heavy chain (CYBB) | in vitro E.coli expression system |
CSB-YP836099BTE1 CSB-EP836099BTE1 CSB-BP836099BTE1 CSB-MP836099BTE1 CSB-EP836099BTE1-B |
Recombinant Bison bison Cytochrome b-245 heavy chain (CYBB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CYBB Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF006325BO | Recombinant Bovine Cytochrome b-245 heavy chain (CYBB) | in vitro E.coli expression system |
CSB-YP006325BO1 CSB-EP006325BO1 CSB-BP006325BO1 CSB-MP006325BO1 CSB-EP006325BO1-B |
Recombinant Bovine Cytochrome b-245 heavy chain (CYBB), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |