Fish Insulin-like growth factors 1,IGF-1 ELISA Kit

Fish Insulin-like growth factors 1,IGF-1 ELISA Kit

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igf1 Proteins

igf1 Proteins

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Fish Insulin-like growth factors 1,IGF-1 ELISA Kit

Specification
Target

Code

CSB-E12122Fh

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q02815

Lead Time

3-5 working days

Abbreviation

IGF-1

Target Name

Insulin-like growth factors 1,IGF-1

Species

Fish

Sample Types

serum, plasma, tissue homogenates

Detection Range

25 pg/mL-2000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	95
	Range %	91-99
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	82-93

Recovery

The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	88	82-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.341	0.343	0.342
750	0.476	0.484	0.480
375	0.682	0.636	0.659
125	1.067	1.072	1.070
25	1.488	1.567	1.528
0	1.955	1.995	1.975

References

A temperature shift on the migratory route similarly impairs hypo-osmoregulatory capacities in two strains of Atlantic salmon (Salmo salar L.) smolts Bernard B,Fish Physiology and Biochemistry,2019
Enteric, hepatic and muscle tissue development of juvenile dourado Salminus brasiliensis fed with lyophilized bovine colostrum Wiolene Montanari Nordi. et al,Fisheries Science,2016
THE EFFECTS OF PESTICIDE NEEMAZAL ON THE GROWTH HORMONE (GH) AND INSULIN-LIKE GROWTH HORMONE (IGF-1) OF CARP FISH (Cyprinus Tihomir Dovramadjiev.et al,researchgate,2016
Growth response, body composition, plasma metabolites, digestive and antioxidant enzymes activities of Siberian sturgeon (Acipenser baerii, Brandt, 1869) fed different dietary protein and carbohydrate: lipid ratio Babaei S.et al,Aquaculture Research,2016
Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacu Piaractus mesopotamicus (Holmberg 1887) fed with lyophilized bovine colostrum Wiolene Montanari Nordi. et al,Aquaculture Research ,2015
Effects of recombinant growth hormone on growth factor and immune component levels in the cinnamon clownfish, Amphiprion melanopus Na Na Kim et al,Marine Biology Research,2014
Molecular cloning, expression analysis of insulin-like growth factor I (IGF-I) gene and IGF-I serum concentration in female and male Tongue sole (Cynoglossus semilaevis) /,Brain Research,2011

Still Have Questions?

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Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
Subcellular Location	Secreted
Protein Families	Insulin family
Database Links	UniGene: Omy.7104
Pathway	HIF-1 signaling pathway MAPK signaling pathway mTOR signaling pathway p53 signaling pathway PI3K-Akt signaling pathway Focal adhesion Ras signaling pathway Signaling pathways regulating pluripotency of stem cells AMPK signaling FoxO signaling pathway Rap1 signaling pathway

Q&A

I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!

Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw cycle.Regarding the later procedures,pls follow the manual.
If any further questions,pls let me know.

Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!

Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.

In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?

Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.

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Check Code：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.