Fish Insulin-like growth factors 1,IGF-1 ELISA Kit

Code CSB-E12122Fh
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q02815
Lead Time 3-5 working days
Abbreviation IGF-1
Target Name Insulin-like growth factors 1,IGF-1
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-2000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 87  
Range % 82-93  
Recovery
The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 88 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.341 0.343 0.342    
750 0.476 0.484 0.480    
375 0.682 0.636 0.659    
125 1.067 1.072 1.070    
25 1.488 1.567 1.528    
0 1.955 1.995 1.975    
References
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Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
Subcellular Location Secreted
Protein Families Insulin family
Database Links

UniGene: Omy.7104

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway

Q&A and Customer Reviews

Q&A
Q:

I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!

A:
Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw cycle.Regarding the later procedures,pls follow the manual.
If any further questions,pls let me know.
Q:

Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!

A:
Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
Q:

In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?

A:
Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.
Customer Reviews

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