| Code |
CSB-E11718h |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
P15692
|
| Lead Time |
3-5 working days |
| Abbreviation |
VEGFA |
| Protein Biological Process 1 |
Cytokine |
| Target Name |
vascular endothelial growth factor A |
| Alias |
RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor |
| Species |
Homo sapiens (Human) |
| Protein Biological Process 3 |
Angiogenesis |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
25.297 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Target Details |
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. |
| HGNC |
12680 |
| RGD |
619991 |
| MGI |
103178 |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
98 |
|
| Range % |
94-106 |
|
| 1:2 |
Average % |
93 |
|
| Range % |
86-97 |
|
| 1:4 |
Average % |
87 |
|
| Range % |
82-93 |
|
| 1:8 |
Average % |
96 |
|
| Range % |
91-105 |
|
|
| Recovery |
| The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-93 |
|
| EDTA plasma (n=4) |
92 |
90-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2000 |
2.651 |
2.751 |
2.701 |
2.547 |
|
| 1000 |
1.729 |
1.829 |
1.779 |
1.625 |
|
| 500 |
1.067 |
1.087 |
1.077 |
0.923 |
|
| 250 |
0.579 |
0.589 |
0.584 |
0.430 |
|
| 125 |
0.372 |
0.382 |
0.377 |
0.223 |
|
| 62.5 |
0.255 |
0.265 |
0.260 |
0.106 |
|
| 31.25 |
0.207 |
0.209 |
0.208 |
0.054 |
|
| 0 |
0.153 |
0.154 |
0.154 |
|
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|
| References |
-
Inhibiting tumour metastasis by DQA modified paclitaxel plus ligustrazine micelles in treatment of non-small-cell lung cancer Xie HJ, et al,Artificial Cells Nanomedicine and Biotechnology,2019
-
A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro Wang L,Molecular Medicine Reports,2019
-
Three kinds of corneal host cells contribute differently to corneal neovascularization Yu H,EBioMedicine,2019
-
The effect of luteal GnRH antagonist on moderate and severe early ovarian hyperstimulation syndrome during in vitro fertilization treatment: a prospective cohort study Zeng C, et al,Archives of Gynecology and Obstetrics,2019
-
MicroRNA‑576‑3p inhibits the migration and proangiogenic abilities of hypoxia‑treated glioma cells through hypoxia‑inducible factor‑1α Qing Hu, et al,International Journal of Molecular Medicine,2019
-
Expression of platelet-derived growth factor-C in aqueous humor of patients with neovascular glaucoma and its correlation with vascular endothelial growth factor Li Y, et al,European journal of ophthalmology,2019
-
Esculetin enhances the inhibitory effect of 5-Fluorouracil on the proliferation, migration and epithelial-mesenchymal transition of colorectal cancer Yan L, et al,Cancer Biomark,2019
-
Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes Liu HN, et al,International Journal of Ophthalmology,2018
-
Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions Ali Abdulqader, et al,Asian Pacific Journal of Tropical Biomedicine,2018
-
Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124 Yin D, et al,Cellular Physiology and Biochemistry,2018
-
Synergistic antiproliferative effects of curcumin and celecoxib in hepatocellular carcinoma HepG2 cells Fatma M.et al,Naunyn-Schmiedeberg's Archives of Pharmacology,2018
-
A randomized controlled trial comparing electroacupuncture with manual acupuncture for motor function recovery after ischemic stroke Chaobo Zhen .et al,European Journal of Integrative Medicine,2018
-
Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification Qingzhen Zhou.et al,Royal Society of Chemistry,2018
-
Assessment of vascular endothelial growth factor in systemic lupus erythematosus patients with anti-phospholipid syndrome Iman I.El-Gazzar.et al,The Egyptian Rheumatologist,2018
-
Fibroblast activation protein in osteosarcoma cells promotes angiogenesis via AKT and ERK signaling pathways Chao Zeng.et al,Oncology Letters,2018
-
Correlation of Plasma Vascular Endothelial Growth Factor and Endostatin Levels with Symptomatic Intra-and Extracranial Atherosclerotic Stenosis in a Chinese Han Yu F.et al,J Stroke Cerebrovasc Dis. ,2017
-
IL-17 induces reactive astrocytes and up-regulation of vascular endothelial growth factor (VEGF) through JAK/STAT signaling You T.et al,Sci Rep. ,2017
-
Differing Expression of Cytokines and TumorMarkers in Combined Pulmonary Fibrosis and Emphysema compared to Emphysema and Pulmonary Fibrosis Xu Ling.et al,COPD: Journal of Chronic Obstructive Pulmonary Disease ,2017
-
Vitreous and plasma changes of endothelin-1, adrenomedullin and vascular endothelium growth factor in patients with proliferative diabetic retinopathy Vingolo EM.et al,Eur Rev Med Pharmacol Sci.,2017
-
Serum vascular endothelial growth factor in Iranian patients with moderate-severe psoriasis before and after treatment: a PASI-75 response as a practical treatment goal Mohammad Shahidi-Dadras.et al,Iranian Journal of Dermatolog,2017
-
Angiogenic effects of low-intensity cathodal direct current on ischemic diabetic foot ulcers: A randomized controlled trial Mohammad Reza Asadi.et al,Diabetes Res Clin Pract.,2017
-
Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia-inducible factor 1a in human dermal fibroblasts Hui SongCui.et al,Archives of Biochemistry and Biophysics,2017
-
Exercise training with dietary restriction enhances circulating irisin level associated with increasing endothelial progenitor cell number in obese adults: an intervention study Huang J.et al,PeerJ.,2017
-
Levels of Serum 25(OH)VD3, HIF-1伪, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio Ying Shao.et al,Diabetes Research,2016
-
Levels of Serum 25(OH)VD3, HIF-1a, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio. Shao Y.et al,J Diabetes Res.,2016
-
Correlation between vascular endothelial growth factor and subclinical atherosclerosis in patients with psoriasis Shahidi-Dadras M. et al,Int J Dermatol.,2015
-
Angiogenic factors are associated with development of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015
-
Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model Lin.et al,Molecular Medicine Reports,2015
-
Balanced oxidative status by nesfatin-1 in intestinal ischemia-reperfusion Ceylan Ayada. et al,Int J Clin Exp Med,2015
-
Association of gene polymorphism with serum levels of inflammatory and angiogenic factors in Pakistani patients with age-related macular degeneration Ambreen F. et al,Mol Vis,2015
-
Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-a, and angiogenesis by blocking endothelial functions. Umar MI et al,Clinics,2014
-
Epigenetic silencing of miR-126 contributes to tumor invasion and angiogenesis in colorectal cancer Zhang Y et al,Oncol Rep,2013
-
Propranolol given orally for proliferating infantile haemangiomas: analysis of efficacy and serological changes in vascular endothelial growth factor and endothelial nitric oxide synthase in 35 patients Yuan WL et al,Br J Oral Maxillofac Surg,2013
-
MicroRNA-26a suppresses angiogenesis in human hepatocellular carcinoma by targeting HGF-cMet pathway Yang X et al,Hepatology,2013
-
The biological effects and mechanisms of calcitonin gene-related peptide on human endothelial cell Tuo Y et al,J Recept Signal Transduct Res.,2013
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The Role of Insulin-Like Growth Factor I and Hypoxia Inducible Factor 1a in Vascular Endothelial Growth Factor Expression in Type 2 Diabetes Jiang F et al,Ann Clin Lab Sci,2013
-
The Association of Serum Vascular Endothelial Growth Factor and Ferritin in Diabetic Microvascular Disease Guo L et al,Diabetes Technol Ther,2013
-
Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis Tang YT et al,Leuk Res,2012
-
Preparation and characterization ofDecellularized tendon slices for tendon tissue engineering /,journal of biomedical materials research,2012
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