Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

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Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

Specification
Target

Code

CSB-E11718h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P15692

Lead Time

3-5 working days

Abbreviation

VEGFA

Protein Biological Process 1

Cytokine

Target Name

vascular endothelial growth factor A

Alias

RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor

Species

Homo sapiens (Human)

Protein Biological Process 3

Angiogenesis

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

25.297 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

HGNC

12680

RGD

619991

MGI

103178

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	93
	Range %	86-97
1:4	Average %	87
	Range %	82-93
1:8	Average %	96
	Range %	91-105

Recovery

The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.651	2.751	2.701	2.547
1000	1.729	1.829	1.779	1.625
500	1.067	1.087	1.077	0.923
250	0.579	0.589	0.584	0.430
125	0.372	0.382	0.377	0.223
62.5	0.255	0.265	0.260	0.106
31.25	0.207	0.209	0.208	0.054
0	0.153	0.154	0.154

References

Preparation and characterization ofDecellularized tendon slices for tendon tissue engineering /,journal of biomedical materials research,2012 Jun;100(6):1448-56.
Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis Tang YT et al,Leuk Res,2013 Apr;37(4):359-66.
The Association of Serum Vascular Endothelial Growth Factor and Ferritin in Diabetic Microvascular Disease Guo L et al,Diabetes Technol Ther,2013 Nov 26.
The Role of Insulin-Like Growth Factor I and Hypoxia Inducible Factor 1a in Vascular Endothelial Growth Factor Expression in Type 2 Diabetes Jiang F et al,Ann Clin Lab Sci,2013 Winter;43(1):37-44.
The biological effects and mechanisms of calcitonin gene-related peptide on human endothelial cell Tuo Y et al,J Recept Signal Transduct Res.,2013 Apr;33(2):114-23.
MicroRNA-26a suppresses angiogenesis in human hepatocellular carcinoma by targeting HGF-cMet pathway Yang X et al,Hepatology,2013 Nov 21.
Propranolol given orally for proliferating infantile haemangiomas: analysis of efficacy and serological changes in vascular endothelial growth factor and endothelial nitric oxide synthase in 35 patients Yuan WL et al,Br J Oral Maxillofac Surg,2013 Jan 3.pii: S0266-4356(12)00623-7
Epigenetic silencing of miR-126 contributes to tumor invasion and angiogenesis in colorectal cancer Zhang Y et al,Oncol Rep,2013 Jul 23.
Ethyl-p-methoxycinnamate isolated from kaempferia galanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-a, and angiogenesis by blocking endothelial functions. Umar MI et al,Clinics,2014 Feb;69(2):134-44.
Association of gene polymorphism with serum levels of inflammatory and angiogenic factors in Pakistani patients with age-related macular degeneration Ambreen F. et al,Mol Vis,2015 Aug 29;21:985-99. eCollection 2015 .
Balanced oxidative status by nesfatin-1 in intestinal ischemia-reperfusion Ceylan Ayada. et al,Int J Clin Exp Med,2015 Mar 15;8(3):3318-24. eCollection 2015 .
Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model Lin.et al,Molecular Medicine Reports,2015 1070-1076
Angiogenic factors are associated with development of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation Nie DM. et al,J Huazhong Univ Sci Technolog Med Sci,2015 35(5):694-699
Correlation between vascular endothelial growth factor and subclinical atherosclerosis in patients with psoriasis Shahidi-Dadras M. et al,Int J Dermatol.,2015 Jul 1.
Levels of Serum 25(OH)VD3, HIF-1a, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio. Shao Y.et al,J Diabetes Res.,/
Levels of Serum 25(OH)VD3, HIF-1伪, VEGF, vWf, and IGF-1 and Their Correlation in Type 2 Diabetes Patients with Different Urine Albumin Creatinine Ratio Ying Shao.et al,Diabetes Research,2016 ,7
Exercise training with dietary restriction enhances circulating irisin level associated with increasing endothelial progenitor cell number in obese adults: an intervention study Huang J.et al,PeerJ.,Aug 14
Low temperature plasma induces angiogenic growth factor via up-regulating hypoxia-inducible factor 1a in human dermal fibroblasts Hui SongCui.et al,Archives of Biochemistry and Biophysics,Jul 24
Angiogenic effects of low-intensity cathodal direct current on ischemic diabetic foot ulcers: A randomized controlled trial Mohammad Reza Asadi.et al,Diabetes Res Clin Pract.,Mar 21;127:147-155.
Serum vascular endothelial growth factor in Iranian patients with moderate-severe psoriasis before and after treatment: a PASI-75 response as a practical treatment goal Mohammad Shahidi-Dadras.et al,Iranian Journal of Dermatolog,Aug
Vitreous and plasma changes of endothelin-1, adrenomedullin and vascular endothelium growth factor in patients with proliferative diabetic retinopathy Vingolo EM.et al,Eur Rev Med Pharmacol Sci.,Feb;21(4):662-668.
Differing Expression of Cytokines and TumorMarkers in Combined Pulmonary Fibrosis and Emphysema compared to Emphysema and Pulmonary Fibrosis Xu Ling.et al,COPD: Journal of Chronic Obstructive Pulmonary Disease ,Jan 27
IL-17 induces reactive astrocytes and up-regulation of vascular endothelial growth factor (VEGF) through JAK/STAT signaling You T.et al,Sci Rep. ,Mar 10
Correlation of Plasma Vascular Endothelial Growth Factor and Endostatin Levels with Symptomatic Intra-and Extracranial Atherosclerotic Stenosis in a Chinese Han Yu F.et al,J Stroke Cerebrovasc Dis. ,Feb 8
Fibroblast activation protein in osteosarcoma cells promotes angiogenesis via AKT and ERK signaling pathways Chao Zeng.et al,Oncology Letters,Feb 12
Assessment of vascular endothelial growth factor in systemic lupus erythematosus patients with anti-phospholipid syndrome Iman I.El-Gazzar.et al,The Egyptian Rheumatologist,7 April
Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification Qingzhen Zhou.et al,Royal Society of Chemistry,July 19
A randomized controlled trial comparing electroacupuncture with manual acupuncture for motor function recovery after ischemic stroke Chaobo Zhen .et al,European Journal of Integrative Medicine,August 20
Synergistic antiproliferative effects of curcumin and celecoxib in hepatocellular carcinoma HepG2 cells Fatma M.et al,Naunyn-Schmiedeberg's Archives of Pharmacology,August 28
Pro-Angiogenic Role of LncRNA HULC in Microvascular Endothelial Cells via Sequestrating miR-124 Yin D, et al,Cellular Physiology and Biochemistry,November 9
Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes Liu HN, et al,International Journal of Ophthalmology,December 18
Gac fruit extracts ameliorate proliferation and modulate angiogenic markers of human retinal pigment epithelial cells under high glucose conditions Ali Abdulqader, et al,Asian Pacific Journal of Tropical Biomedicine,December 26

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Function	Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Involvement in disease	Microvascular complications of diabetes 1 (MVCD1)
Subcellular Location	Secreted
Protein Families	PDGF/VEGF growth factor family
Tissue Specificity	Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
Database Links	HGNC: 12680 OMIM: 192240 KEGG: hsa:7422 STRING: 9606.ENSP00000361125 UniGene: Hs.73793
Pathway	HIF-1 signaling pathway MAPK signaling pathway PI3K-Akt signaling pathway VEGF signaling pathway Focal adhesion Ras signaling pathway AGE-RAGE signaling pathway in diabetic complications Rap1 signaling pathway

VEGFA Antibody

VEGFA Protein

VEGFA Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA025833GA01HU	VEGFA Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA004762	VEGFA Antibody	Human	WB,ELISA
CSB-PA060064	VEGFA Antibody	Human,Mouse,Rat	IHC,ELISA
CSB-PA379558	VEGFA Antibody	Human	ELISA,WB,IHC
CSB-PA793155	VEGFA Antibody	Human	ELISA,WB
CSB-PA07529D0Rb	VEGFA Antibody, Biotin conjugated	Human	ELISA
CSB-PA07529C0Rb	VEGFA Antibody, FITC conjugated	Human	ELISA
CSB-PA07529B0Rb	VEGFA Antibody, HRP conjugated	Human	ELISA

VEGFA Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA07529A0Rb	VEGFA Antibody	Human	ELISA, WB

VEGFA Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP025833HU CSB-EP025833HU CSB-BP025833HU CSB-MP025833HU	Recombinant Human Vascular endothelial growth factor A(VEGFA)	Yeast E.coli Baculovirus Mammalian cell
CSB-AP002601HU	Recombinant Human Vascular endothelial growth factor A protein(VEGFA) (Active)	E.coli
CSB-AP002591HU	Recombinant Human Vascular endothelial growth factor A protein(VEGFA) (Active)	Yeast

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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