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| Code | CSB-E11718h |
| Size | 96T,5×96T,10×96T |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-106 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-97 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-105 | |||||
| The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 92 | 90-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The human VEGF ELISA Kit is based on the quantitative sandwich ELISA technology. Capture antibody and detection antibody sandwich the analyte to form an immune complex, HRP of which catalyzes the TMB to generate a colored product. The density of produced color is proportional to the human VEGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. It can be used to detect human VEGF in serum, plasma, cell culture supernates, tissue homogenates, cell lysates, or urine.
VEGFA, commonly referred to as VEGF, is an essential growth factor for vascular endothelial cells and regulates endothelial cell permeability, proliferation, migration, and fenestration formation. It is also a central proangiogenic factor in the development, wound healing, bone formation, hematopoiesis, and pathogenic processes, such as carcinogenesis, psoriasis, and rheumatoid arthritis. VEGF binds to VEGFRs and activates multiple signaling pathways involving cell proliferation, survival, migration, and tissue permeability. High VEGF expression has been detected in tumor cells and tumor-related stromal cells, allowing tumor neoangiogenesis and facilitating tumoral immune tolerance.
Sample type: Cell culture supernatant
Sample species: Human
Review: The concentrations of VEGF-A in fibroblast cell culture supernatants 6 h and 24 h after LTP treatment for 30 s, 1 min, 3 min, and 5 min were measured by ELISA. Each sample was assayed in duplicate, and the analysis was performed three times independently. Data are the mean ± S.E. *p < 0.05 vs. the untreated control group.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: Expression levels of VEGF in the conditioned medium of the indicated cells (U87 and U251) treated with hypoxia for 24 h, as determined by an ELISA.
By Anonymous
Sample type: Serum
Sample species: Human
Review: There was a significant tendency for an increase in serum VEGF during the course from day 3 POR to day 6 POR in the control group (P=0.015). In the cetrorelix group, no significant increase or decline in VEGF from day 3 POR to day 6 POR was observed.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: The decrease in VEGF-A subsequent to FAP silencing was also confirmed by ELISA. *P<0.05 vs. control.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: Effects of extracts from Gac fruit parts (peel, pulp, seed, and aril) on VEGF level of ARPE-19 cells in HG condition treated with different concentrations (50, 100, and 250 μg/mL) for 48 h. Results are expressed as mean ± SD for three measurements. Bars not having the same letters are significantly different at P < 0.05. LG: low glucose (5.5 mmol/L); HG: high glucose (30 mmol/L).
By Anonymous
Sample type: Serum
Sample species: Human
Review: Expression of serum VEGF in HC, NDR, NPDR and PDR groups a P<0.05 versus HC group; b P<0.05 versus NDR group; c P<0.05 versus NPDR group.
By Anonymous
