Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

Code CSB-E11718h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P15692
Lead Time 3-5 working days
Abbreviation VEGFA
Protein Biological Process 1 Cytokine
Target Name vascular endothelial growth factor A
Alias RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Species Homo sapiens (Human)
Protein Biological Process 3 Angiogenesis
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates, urine
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 25.297 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
HGNC 12680
RGD 619991
MGI 103178
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 94-106  
1:2 Average % 93  
Range % 86-97  
1:4 Average % 87  
Range % 82-93  
1:8 Average % 96  
Range % 91-105  
Recovery
The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 92 90-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.651 2.751 2.701 2.547  
1000 1.729 1.829 1.779 1.625  
500 1.067 1.087 1.077 0.923  
250 0.579 0.589 0.584 0.430  
125 0.372 0.382 0.377 0.223  
62.5 0.255 0.265 0.260 0.106  
31.25 0.207 0.209 0.208 0.054  
0 0.153 0.154 0.154    
References
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Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Involvement in disease Microvascular complications of diabetes 1 (MVCD1)
Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
Database Links

HGNC: 12680

OMIM: 192240

KEGG: hsa:7422

STRING: 9606.ENSP00000361125

UniGene: Hs.73793

Pathway HIF-1 signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Ras signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Rap1 signaling pathway

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