Monkey Insulin-like Growth Factor 1(IGF-1) ELISA KIT

Code CSB-E13401Mk
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q68LC0
Lead Time 3-5 working days
Abbreviation IGF-1
Target Name Insulin-like Growth Factor 1(IGF-1)
Species Monkey
Sample Types serum, plasma
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
40002.063 2.115 2.089 2.000
20001.611 1.642 1.627 1.538
10001.107 1.154 1.131 1.042
5000.725 0.698 0.712 0.623
2500.398 0.375 0.387 0.298
1250.296 0.286 0.291 0.202
62.50.188 0.179 0.184 0.095
00.086 0.091 0.089
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Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular Location Secreted
Protein Families Insulin family
Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway

Q&A and Customer Reviews

Q&A
Q:

Can you please let me know if this kit was tested for Rhesus Monkey?

A:
Thanks for your inquiry.
CSB-E13401Mk
This kit is designed against Rhesus Monkey.
We tested undiluted serum and plasma samples before and the test value is 1000ng/ml.
Pls let me know if you have any further questions. Thank you.
Q:

Thanks for your response.
What is the dynamic range for concentration in standard protein calibration curve?

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
The detection range of this kit is 62.5 ng/ml-4000 ng/ml.Pls let me know if you have any further questions. Thank you.
Customer Reviews

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