Monkey Insulin-like Growth Factor 1(IGF-1) ELISA KIT

Code CSB-E13401Mk
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Insulin-like Growth Factor 1(IGF-1)
Alternative Names
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation
IGF-1
Uniprot No.
Species
Monkey
Sample Types
serum, plasma
Detection Range
62.5 ng/mL-4000 ng/mL
Sensitivity
15.6 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
40002.063 2.115 2.089 2.000
20001.611 1.642 1.627 1.538
10001.107 1.154 1.131 1.042
5000.725 0.698 0.712 0.623
2500.398 0.375 0.387 0.298
1250.296 0.286 0.291 0.202
62.50.188 0.179 0.184 0.095
00.086 0.091 0.089
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Monkey IGF-1 ELISA Kit was designed for the quantitative measurement of Monkey IGF-1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.

Customer Reviews and Q&A

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 Q&A
Q:

Can you please let me know if this kit was tested for Rhesus Monkey?

A:
Thanks for your inquiry.
CSB-E13401Mk
This kit is designed against Rhesus Monkey.
We tested undiluted serum and plasma samples before and the test value is 1000ng/ml.
Pls let me know if you have any further questions. Thank you.
Q:

Thanks for your response.
What is the dynamic range for concentration in standard protein calibration curve?

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
The detection range of this kit is 62.5 ng/ml-4000 ng/ml.Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Subcellular Location
Secreted.
Protein Families
Insulin family
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