Mouse Interleukin-7,IL-7 ELISA kit

Instructions
Code CSB-E10257m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 7
Alternative Names IL-7, IL7 nirs variant 6
Abbreviation IL7
Uniprot No. P10168
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 100-110
1:2 Average % 88
Range % 84-92
1:4 Average % 107
Range % 100-113
1:8 Average % 83
Range % 80-85
Recovery
The recovery of mouse IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 93-99
EDTA plasma (n=4) 89 85-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.681 2.600 2.641 2.511
100 2.042 2.102 2.072 1.942
50 1.448 1.400 1.424 1.294
25 0.885 0.901 0.893 0.763
12.5 0.552 0.562 0.557 0.427
6.25 0.333 0.341 0.337 0.207
3.12 0.274 0.268 0.271 0.141
0 0.131 0.129 0.130  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Subcellular Location Secreted
Protein Families IL-7/IL-9 family
Database Links

KEGG: mmu:16196

STRING: 10090.ENSMUSP00000126219

UniGene: Mm.3825

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