Mouse Interleukin-7,IL-7 ELISA kit

Instructions
Code CSB-E10257m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 7
Alternative Names Il7 ELISA Kit; Il-7 ELISA Kit; Interleukin-7 ELISA Kit; IL-7 ELISA Kit
Abbreviation IL7
Uniprot No. P10168
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 105
Range % 100-110
1:2 Average % 88
Range % 84-92
1:4 Average % 107
Range % 100-113
1:8 Average % 83
Range % 80-85
Recovery
The recovery of mouse IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 93-99
EDTA plasma (n=4) 89 85-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
200 2.681 2.600 2.641 2.511
100 2.042 2.102 2.072 1.942
50 1.448 1.400 1.424 1.294
25 0.885 0.901 0.893 0.763
12.5 0.552 0.562 0.557 0.427
6.25 0.333 0.341 0.337 0.207
3.12 0.274 0.268 0.271 0.141
0 0.131 0.129 0.130  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Gene References into Functions
  1. We engineered CAR-T cells to express interleukin (IL)-7 and CCL19 (7 x 19 CAR-T cells), as these factors are essential for the maintenance of T-cell zones in lymphoid organs... Following treatment of mice with 7 x 19 CAR-T cells, both recipient conventional T cells and administered CAR-T cells generated memory responses against tumors. PMID: 29505028
  2. A key role of the IL7 and Interferon type I receptor axis in the regulation of intratumoral t-cell functions and in the development of primary breast tumor growth and metastasis. PMID: 29070614
  3. BMP4 and IL7 appear to be involved in the interaction between intestinal epithelial cells and intestinal epithelial cells and in the mechanism underlying intestinal mucosal barrier dysfunction. PMID: 29436597
  4. This finding warrants future development of IL-21 and IL-7 co-expressing whole-cell cancer vaccines and their relevant combinatorial regimens. PMID: 27571893
  5. both Flt3 ligand (FL) and IL-7 regulate B-cell commitment in a permissive manner: FL by inducing proliferation of Ly6D(+)CD135(+)CD127(+)CD19(-) progenitors and IL-7 by providing survival signals to these progenitors PMID: 27911806
  6. the in vivo biological role of m(6)A modification in T-cell-mediated pathogenesis and reveals a novel mechanism of T cell homeostasis and Il-7 signal-dependent induction of mRNA degradation PMID: 28792938
  7. IL-7/IL-7R signaling pathway plays a possible role in recurrent pregnancy loss by upregulating Th17 immunity while downregulating Treg immunity. PMID: 27767237
  8. this study shows that IL-7 homeostasis is achieved through consumption by multiple subsets of innate and adaptive immune cells PMID: 28723549
  9. lymphatic vessel expansion occurs in two distinct phases; the first wave of expansion is dependent on IL-7; the second phase, responsible for leukocyte exit from the glands, is regulated by lymphotoxin (LT)betaR signaling PMID: 27474071
  10. DNM2 mutations cooperate with Lmo2 T-cell oncogenes by enhancing IL-7 signalling. PMID: 27118408
  11. IL-7 responsiveness in RTE is designed to maximize survival at the expense of reduced proliferation, consistent with RTE serving as a subpopulation of T cells rich in diversity but not in frequency. PMID: 27129922
  12. Expression of IL-7 is beneficial for induction of potent and long-lasting humoral immune responses. PMID: 28100620
  13. findings support a redundant role for adaptive Th17 cell- and innate gammadeltaT17 cell-derived IL17 in bacteria-induced colon carcinogenesis, stressing the importance of therapeutically targeting the cytokine itself rather than its cellular sources PMID: 26880802
  14. functional Gimap5 is required for optimal signaling through TCR and IL-7R in T cells. PMID: 27023180
  15. continuous IL7 signaling was not required for tumor regression, although LIP of naive CD8+ T cells is usually regulated by IL7 PMID: 26880265
  16. IL7 represses the follicular helper T cell gene program. PMID: 26743592
  17. This study uncovers the metabolic mechanisms by which IL-7 tailors the metabolism of memory T cells to promote their longevity and fast response to rechallenge. PMID: 25957683
  18. IL-7 reduced IRAK-M expression and attenuated immune tolerance induced by either LPS or CpGA PMID: 26218271
  19. Poly I:C induces IL-7 production, early inflammatory responses, and characteristic pathologies of SS-like dacryoadenitis in non-autoimmune-prone C57BL/6 mice. PMID: 26658504
  20. Data show that interleukin 7 (IL-7) signaling is a prerequisite for optimal CD4(+) T cell activation. PMID: 26319414
  21. reduced surface expression of IL-7R and concomitant limited responsiveness to IL-7 signals as a common mechanism resulting in reduced cell survival of common lymphoid progenitors and thymocytes at the double-negative to double-positive transition PMID: 26475928
  22. Hair follicle expression of IL-7 was required for CD8+ and CD4+ skin-resident memory T (TRM) cells to exert tropism for the epidermis. In a cutaneous T cell lymphoma model, CD4+ TRM lymphoma cell localization depended on hair follicle-derived IL-7. PMID: 26479922
  23. Data show the contribution of IL-23/IL-23 receptor and IL-7/IL-7 receptor signaling in Th17 and Th1 cell dynamics during experimental autoimmune encephalomyelitis (EAE). PMID: 26223651
  24. IL-7 plays a major role in innate immunity against Citrobacter rodentium infection. PMID: 26034215
  25. The effects of 6-formylindolo (3,2-b) carbazole (Ficz), a ligand of aryl hydrocarbon receptor, on IL-7 expression, colitis and lymphocyte phenotypes are reported. PMID: 25799939
  26. IL-7 critically acts cooperatively with signaling via the pre-TCR and Notch1 to coordinate proliferation, differentiation and TCRalpha recombination during beta-selection. PMID: 25729925
  27. The enhanced thymic reconstitution in the rIL-7/HGFbeta-treated allogeneic BMT recipients results in increased number and functional activities of peripheral T cells. PMID: 24349415
  28. these results suggest that PU.1 and Spi-B activate Btk to oppose IL-7 responsiveness in developing B cells. PMID: 25505273
  29. Results provided evidence that IL-7/IL-7R induce VEGF-D upregulation and promote lymphangiogenesis via c-Fos/c-Jun pathway in lung cancer. PMID: 24115038
  30. OX40 and IL-7 play synergistic, but distinct roles in the homeostatic proliferation of CD4(+) effector memory T cells PMID: 25103720
  31. IL-7 holds promise as a critical cytokine for selectively inducing Tfh cell generation. PMID: 24899182
  32. NF-kappaB has a role in controlling IL-7 responsiveness of quiescent naive T cells PMID: 24799710
  33. IL-12 induces the expression of IL-7 in microglia and macrophages via both IL-12Rbeta2 and IL-12Rbeta1. PMID: 24224652
  34. Ikaros is a central regulator of IL-7 signaling and pre-B cell development PMID: 24297995
  35. our data point toward an unexpected new role for IL-7 as a potential autocrine mediator of lymphatic drainage PMID: 23963040
  36. KGF could up-regulate IL-7 expression through the STAT1/IRF-1, IRF-2 signaling pathway, which is a new insight in potential effects of KGF on the intestinal mucosal immune system. PMID: 23554911
  37. -7 enhances the Th1 response to promote the development of Sjogren's syndrome-like autoimmune exocrinopathy in mice. PMID: 23666710
  38. IRFs activated by lymphocyte adhesion induce IL-7 transcription through ISRE in stromal cells. PMID: 23376291
  39. our results suggest that thymic epithelial cell-derived IL-7 plays a major role in proliferation, survival, and maturation of thymocytes and is indispensable for gammadelta T cell development PMID: 23686483
  40. We have analyzed the discrete contributions of the antibody constant (Fc) and IL-7-binding (Fab) domains to the mechanism. PMID: 23610371
  41. expression of IL-7/IL-7R is strongly correlated with rheumatoid arthritis activity and ligation of IL-7 to IL-7R contributes to monocyte homing, differentiation of osteoclasts, and vascularization in the collagen-induced arthritis effector phase. PMID: 23606539
  42. IL-7 could be an important mediator in arthritic conditions PMID: 22676399
  43. Cessation of the IL-7 response of pre-B cell signaling components is controlled via a cell-autonomous mechanism that operates at a discrete developmental transition marked by transient expression of c-Myc. PMID: 23420891
  44. Interleukin-7, but not thymic stromal lymphopoietin, plays a key role in the T cell response to influenza A virus. PMID: 23189186
  45. Interleukin-7 supports survival of T-cell receptor-beta-expressing CD4(-) CD8(-) double-negative thymocytes. PMID: 23215679
  46. poly I:C boosts the T cell immune response in the lung by inducing local IL-7 production, which in turn, enhances T cell-derived IFN-gamma to promote macrophage recruitment, CXCR3 ligand expression, and T cell infiltration. PMID: 23271706
  47. Data show that lymphatic endothelial cells (LECs) are a prominent source of IL-7 both in human and murine lymph nodes. PMID: 22955921
  48. This is the first demonstration that high levels of IL-7 antagonize Notch-1 signaling and suggest that IL-7 may affect T- versus B-lineage choice in the thymus. PMID: 22899673
  49. Although IL-7 is crucial for naive CD4+ T cell homeostatic proliferation in response to lymphopenia, it has minimal impact on the homeostatic proliferation of regulatory CD4+ (Treg) cells. PMID: 22933631
  50. These observations establish a key role for IL-7 in the complex mechanisms by which immune mediators modulate metabolic functions. PMID: 22768283
  51. Loss of total Bim in interleukin-7-deprived T-cells resulted in delayed apoptosis. PMID: 22728771
  52. Topical cilomilast suppresses the generation of IL-17-associated immunity in experimental DED. PMID: 22577075
  53. Interleukin-7 links T lymphocyte and intestinal epithelial cell homeostasis PMID: 22384106
  54. JunD/AP-1-mediated gene expression promotes lymphocyte growth dependent on interleukin-7 signal transduction PMID: 22384197
  55. We propose that activity of the IL-7/STAT5 pathway plays a critical protective role in pro-b cells development PMID: 22581861
  56. we document a critical requirement for in vivo IL-7 in T cell maintenance and specifically in Foxp3(+) cell homeostasis. PMID: 22593613
  57. SI-IEL and lamina propria lymphocytes do not express the receptor for IL-7 (IL-7Ralpha/CD127). PMID: 21928282
  58. Different levels of IL-7 signalling in vivo evoked distinct molecular mechanisms to regulate homeostatic fitness. PMID: 21932447
  59. Attenuation of IL-7 signaling resulted in upregulation of the transcription factors Foxo1 and Pax5 PMID: 22267219
  60. IL-7 blocks the differentiation of double-negative 3 thymocytes to double-positive cells PMID: 21882187
  61. IL-7 is critical for the development of the intrinsic function of marginal zone B cells PMID: 21873520
  62. SOCS-1 inhibits IL-7 responses in pre-B cell lines PMID: 21873521
  63. IL-7 is essential for normal development of Ly6D(+) common lymphoid progenitors and Ebf-1 is crucial for lineage restriction in early lymphoid progenitors. PMID: 21652681
  64. matures suppressive CD127(+) forkhead box P3 (FoxP3)(+) T cells into CD127(-) CD25(high) FoxP3(+) regulatory T cells PMID: 21413939
  65. Aging leads to a significantly altered composition of lymphocyte subsets, and the lack of IL-7 seems to accelerate this process. PMID: 21388913
  66. cytokine IL-7 plays an important role in hypothalamic body weight regulation PMID: 20376352
  67. Following syngeneic bone marrow transplantion, administration of hybrid IL-7/hepatocyte growth factorbeta chain supports rapid and complete regeneration of the thymus, reconstituting the naive T cell pool and its normally diverse receptor repertoire. PMID: 21209281
  68. Following proliferation mediated by either self-peptide-histocompatibility (MHC) interaction alone or in combination with IL-7, this study finds that T cell reconstitution is most efficient in the presence of increased IL-7 availability. PMID: 21239710
  69. Post-positive selection signaling by IL-7 through mitogen-activated protein kinase ERK5 leads to the expression of Kruppel-like factor KLF2 and resulting emigration of T cells from the thymus. PMID: 21187442
  70. IL-7 drives Cdc25A-mediated T-cell proliferation, which prevents the nuclear translocation of Foxo1, leading to reduced expression of CD62L and the migration of T cells into circulation. PMID: 20831893
  71. bone-invading cells express and produce IL-7, which is known to promote osteoclast activation and osteolytic lesions PMID: 20067635
  72. IL7 and Kit signaling synergize in positively regulating lymphoid tissue cell numbers in lymph node (LN) anlage and in promoting LN organogenesis. PMID: 20709954
  73. gammadelta T cells are actively involved in the generation of alphabetaTCR+IL-17+ T cells in uveitis PMID: 20375337
  74. IL-7-dependent B cells are required for the response to pneumococcal polysaccharide in adults. PMID: 20505146
  75. FLT3 and CD43 signaling pathways involve STAT5A via Fiz1 and Hipk2 in B lymphoblastic lymphomas. PMID: 20360400
  76. our findings identify a negative feedback mechanism in which signals derived from TCRbeta-selected thymocytes modulate Thymic epithelial cell-dependent IL-7 expression PMID: 20439914
  77. Exposure of CD4(+) T cells to IL-7 during priming results in an increased frequency of CD4(+) memory T cells. PMID: 20064560
  78. the IL-7 signaling pathway regulates lymph node organogenesis by controlling the size of the hematopoietic lymphoid tissue inducer cell pool. PMID: 20207995
  79. control of glycolysis by IL-7R may contribute to the well-described roles of IL-7 in T cell development, homeostatic proliferation, and survival. PMID: 20194717
  80. analysis of a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus PMID: 19907640
  81. Results demonstrated that utilizing nonlytic Fc-fused IL-7 (IL-7-Fc(m)) as a genetic adjuvant significantly enhanced not only CD4(+) but also CD8(+) T-cell responses. PMID: 19950168
  82. Findings show that adult LTi-like cells require extrinsic signals from podoplanin(+) splenic stromal cells to survive and suggest that IL-7 is not necessary to mediate their survival in the adult spleen. PMID: 19950181
  83. the maintenance of peripheral T cells by IL-7 occurs partly through inhibition of Bim activity at the posttranslational level. PMID: 19933849
  84. STAT5 and IL-7 signaling control cell survival and the developmental ordering of immunoglobulin gene rearrangements PMID: 19946273
  85. IL-7 regulates chromatin accessibility for V(D)J recombination by specifically altering histone acetylation within the T cell receptor gamma locus. PMID: 11714763
  86. enhances the survival and maintains the size of naive T cells PMID: 11739504
  87. IL7 activates the nuclear localizing signal in SHP-1, increasing nuclear localization of SHP-1, which, in turn, regulates nuclear signaling in cell growth. PMID: 11987243
  88. role in regulating homeostatic proliferation of memory phenotype CD8+ cells in conjunction with IL-15 PMID: 12070280
  89. overexpression leads to IL-15-independent generation of memory phenotype CD8+-T cells PMID: 12070281
  90. PPBSF is the operative form of IL-7 that normally induces IL-7Ralpha(lo) pre-pro-B cells to proliferate and differentiate into IL-7Ralpha(hi) pro-B cells, which then proliferate and differentiate into pre-B cells on stimulation with monomeric IL-7. PMID: 12184921
  91. The earliest step in B lineage differentiation from common lymphoid progenitors is critically dependent upon interleukin 7. PMID: 12208884
  92. soluble factors produced by the thymic microenvironment affect the ability of pro-B cells to respond optimally to IL-7, constituting a specific inhibition of intrathymic B lymphopoiesis PMID: 12393710
  93. IL-7 induces substantial expression of latent HIV while having minimal effects on the T-cell phenotype. PMID: 12438635
  94. Increased production uncouples bone formation from bone resorption during estrogen deficiency PMID: 12464669
  95. Before kappa chain rearrangement occurs, TRANCE together with IL-7 induces pre-B cells to proliferate and makes this rearrangement more efficient. PMID: 12548564
  96. Transient IL-7/IL-7R signaling provides a mechanism for feedback inhibition of immunoglobulin heavy chain gene rearrangements. PMID: 12594950
  97. Study with transgenic IL-7(-/-) mice reveal that although IL-7 is not required for TCR gamma delta cell proliferation, it is required to prolong the life span of mature TCR gamma delta cells. PMID: 12682238
  98. Tyrosine phosphorylation of clathrin heavy chain was strongly induced by interleukin 7(IL-7). PMID: 14636898
  99. the progeny derived from myeloid-biased hematopoietic stem cells failed to respond to IL-7 in vitro. PMID: 14976059
  100. Pattern of sensitivity of developing thymocytes to IL-7 suggests a two-signal mechanism by which survival of thymocytes between the TCR beta-chain rearrangement process (beta-selection) and the positive selection checkpoint is governed. PMID: 15034036

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Subcellular Location Secreted
Protein Families IL-7/IL-9 family
Database Links

KEGG: mmu:16196

STRING: 10090.ENSMUSP00000126219

UniGene: Mm.3825

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