Mouse Interleukin-7,IL-7 ELISA kit

Product Details

Target Name

interleukin 7

Alternative Names

IL-7, IL7 nirs variant 6

Abbreviation

IL7

Uniprot No.

P10168

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	105
	Range %	100-110
1:2	Average %	88
	Range %	84-92
1:4	Average %	107
	Range %	100-113
1:8	Average %	83
	Range %	80-85

Recovery

The recovery of mouse IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	89	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.681	2.600	2.641	2.511
100	2.042	2.102	2.072	1.942
50	1.448	1.400	1.424	1.294
25	0.885	0.901	0.893	0.763
12.5	0.552	0.562	0.557	0.427
6.25	0.333	0.341	0.337	0.207
3.12	0.274	0.268	0.271	0.141
0	0.131	0.129	0.130

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Target Data

Function	Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
Subcellular Location	Secreted
Protein Families	IL-7/IL-9 family
Database Links	KEGG: mmu:16196 STRING: 10090.ENSMUSP00000126219 UniGene: Mm.3825

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Check Code：

Code	CSB-E10257m
Size	96T,5×96T,10×96T
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Mouse Interleukin-7,IL-7 ELISA kit

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