Code | CSB-E10257m |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
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Trial Size |
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 105 | ||||
Range % | 100-110 | |||||
1:2 | Average % | 88 | ||||
Range % | 84-92 | |||||
1:4 | Average % | 107 | ||||
Range % | 100-113 | |||||
1:8 | Average % | 83 | ||||
Range % | 80-85 |
The recovery of mouse IL-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 93-99 | ||||
EDTA plasma (n=4) | 89 | 85-93 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
10 | 2.681 | 2.600 | 2.641 | 2.511 | |||
5 | 2.042 | 2.102 | 2.072 | 1.942 | |||
2.5 | 1.448 | 1.400 | 1.424 | 1.294 | |||
1.25 | 0.885 | 0.901 | 0.893 | 0.763 | |||
0.625 | 0.552 | 0.562 | 0.557 | 0.427 | |||
0.312 | 0.333 | 0.341 | 0.337 | 0.207 | |||
0.156 | 0.274 | 0.268 | 0.271 | 0.141 | |||
0 | 0.131 | 0.129 | 0.130 |
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This Mouse IL7 ELISA Kit was designed for the quantitative measurement of Mouse IL7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/ml - 10 ng/ml and the sensitivity is 0.039 ng/ml.
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