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Rat soluble cluster of differentiation 14,sCD14 ELISA Kit

Citations (1) Protocol
Code CSB-E11178r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name CD14 molecule
Alternative Names Cd14Monocyte differentiation antigen CD14 ELISA Kit; Myeloid cell-specific leucine-rich glycoprotein ELISA Kit; CD antigen CD14 ELISA Kit
Abbreviation CD14
Uniprot No. Q63691
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 28 ng/mL-1800 ng/mL
Sensitivity 7 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %86-100
1:2Average %99
Range %90-103
1:4Average %89
Range %83-92
1:8Average %95
Range %84-98
Recovery
The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10294-108
EDTA plasma (n=4)9689-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
18002.530 2.401 2.466 2.283
9002.066 1.986 2.026 1.843
4501.311 1.356 1.334 1.151
2250.759 0.779 0.769 0.586
112.50.456 0.478 0.467 0.284
560.352 0.344 0.348 0.165
280.271 0.263 0.267 0.084
00.181 0.185 0.183
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
Gene References into Functions
  1. This study shows that sCD14 impairs contractile function and insulin action in primary adult rat cardiomyocytes. PMID: 27816522
  2. CD 14 mRNA expression increased significantly after five days of bile duct ligation in liver, lung, spleen, and ileum. PMID: 25093541
  3. biliary drainage affects expression of inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats PMID: 23613625
  4. Burn injury-induced mitochondrial damage in cardiomyocytes was prevented in CD14 knockout mice. Burn injury produces CD14-dependent mitochondrial damage via oxidative stress in myocardium. PMID: 20348223
  5. Acute cerebral hemorrhage results in endotoxemia and widespread increases in CD14 and IL-1beta expression. PMID: 19534684
  6. Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia. PMID: 12046090
  7. Increase of CD14 mRNA expression may result in greater sensitivity to endotoxin and thus lead to liver injury. PMID: 12113681
  8. Although microglial-derived CD14 does not contribute to early events triggered by intrastriatal endotoxin treatment, it modulates the duration of inflammation within the cerebral tissue. PMID: 12218159
  9. Ethanol administration led to a significant synthesis of endotoxin receptor CD14 protein and its gene expression in Kupffer cells, which maybe result in the pathological changes of liver tissue and hepatic functional damages. PMID: 12632533
  10. the CD14 antigen system appeared to be an early trigger for Kupffer cell activation followed by induction of inflammatory cytokine interleukin-1beta synthesis leading to hepatic injury after partial hepatectomy PMID: 14572767
  11. CD14 glycoprotein is present on the cell membranes of vascular smooth muscle cells of rat aorta, where it acts as a lipopolysaccharide receptor. PMID: 15153652

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Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus.
Tissue Specificity Detected in macrophages and peripheral blood monocytes.
Database Links

KEGG: rno:60350

STRING: 10116.ENSRNOP00000023977

UniGene: Rn.42942
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