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| Code | CSB-E11178r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-100 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 90-103 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-92 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-98 | |||||
| The recovery of rat sCD14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 94-108 | ||||
| EDTA plasma (n=4) | 96 | 89-101 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E11178r is a sandwich ELISA kit developed to measure concentrations of rat sCD14 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of sCD14 in samples. The color intensity is measured at 450 nm via a microplate reader.
CD14 is a GPI-anchored receptor expressed by macrophages (MΦ) and neutrophils, facilitating pro- and anti-inflammatory cytokine production in response to various DAMPs. It functions in the TLR4-MD2 complex to initiate and activate proinflammatory signaling events in response to gram-negative bacteria through recognition of lipopolysaccharide (LPS). It plays a critical role in the phagocytic clearance of apoptotic cells and in the reactivation and immune recognition of microbial cell wall components of Gram-positive and Gram-negative bacteria. CD14 has also been reported to be upregulated during renal ischemia-reperfusion injury and in tubular epithelial cells of the kidney after unilateral ureteral obstruction.
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