Recombinant Human Interleukin-15 (IL15) is expressed in E. coli and represents the full length of the mature protein, spanning amino acids 49-162. This tag-free protein is produced with a purity exceeding 95%, as verified by SDS-PAGE analysis. It demonstrates biological activity, with an effective dose (ED50) in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells ranging from 40 to 200 pg/ml. The endotoxin level is maintained below 1.0 EU/µg, as determined by the LAL method.
Interleukin-15 (IL15) appears to be a central player in immune response regulation. This cytokine is particularly important for the proliferation and survival of natural killer (NK) cells and CD8+ T cells, which may contribute significantly to adaptive immunity. Researchers studying immune cell function, cancer immunotherapy, and vaccine development often turn to IL15 for insights into potential therapeutic strategies.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. T Cell Proliferation and Activation Studies
Scientists can use this recombinant IL-15 to explore T cell biology, especially how CD8+ T cells and NK cells proliferate and become activated in laboratory conditions. The biological activity confirmed through CTLL-2 cells - with an ED50 of 40-200 pg/ml - suggests this protein can effectively stimulate cytotoxic T lymphocytes. Research teams might study T cell memory formation, how effector functions develop, or the molecular mechanisms that drive IL-15-mediated signaling. Given its high purity and low endotoxin levels, it appears suitable for sensitive immunological assays where contamination could potentially skew results.
2. Natural Killer Cell Functional Assays
This protein may serve as an important reagent for investigating NK cell development, survival, and cytotoxic capabilities in preclinical research. Since IL-15 appears essential for NK cell homeostasis, researchers could use this recombinant protein to maintain NK cell cultures or boost their activation for functional studies. Scientists might investigate NK cell-mediated cytotoxicity, cytokine production patterns, or how receptor expression changes under different conditions. The defined activity range offers a reasonable starting point for dose-response experiments in NK cell biology.
3. Cytokine Signaling Pathway Analysis
Researchers can potentially use this biologically active IL-15 protein to dissect receptor signaling cascades and downstream molecular events across different cell types. The tag-free design likely ensures that experimental results reflect genuine IL-15 signaling without interference from fusion tags. Scientists could study JAK-STAT pathway activation, track phosphorylation events, or monitor gene expression changes after IL-15 stimulation. The high purity specification supports its use in biochemical assays where precise protein concentrations matter and background interference needs to stay minimal.
4. Antibody Development and Validation
The recombinant IL-15 could work as an antigen for developing and testing anti-IL-15 antibodies in research settings. Since the mature protein sequence (49-162aa) represents the biologically relevant form, it seems appropriate for generating antibodies that recognize native IL-15. Research groups might use this protein in ELISA development, Western blot validation, or antibody specificity testing. The confirmed biological activity also allows functional validation of neutralizing antibodies through competition assays using the established CTLL-2 proliferation method.
5. Immunotherapy Mechanism Studies
Scientists could use this IL-15 protein in preclinical studies exploring immune-based therapeutic approaches, particularly those targeting T cell and NK cell responses. Research teams might study how it combines with other immunomodulatory agents, evaluate immune cell expansion protocols, or investigate why cancer cell lines sometimes develop resistance mechanisms. The low endotoxin content and high purity may make it suitable for complex co-culture experiments involving multiple interacting cell types. These defined activity parameters could provide a foundation for establishing reproducible experimental conditions across different research laboratories.
