GALE Antibody

Code CSB-PA613499HA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: GALE antibody at 2µg/ml
    Lane 1: HepG2 whole cell lysate
    Lane 2: Hela whole cell lysate
    Lane 3: A549 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 39, 31 kDa
    Observed band size: 39 kDa

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA613499HA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA613499HA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA613499HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) GALE Polyclonal antibody
Uniprot No. Q14376
Target Names GALE
Alternative Names FLJ95174 antibody; FLJ97302 antibody; Galactose 4 epimerase UDP antibody; Galactowaldenase antibody; galE antibody; GALE_HUMAN antibody; OTTHUMP00000002991 antibody; OTTHUMP00000002994 antibody; OTTHUMP00000037931 antibody; OTTHUMP00000044857 antibody; SDR1E1 antibody; short chain dehydrogenase/reductase family 1E member 1 antibody; UDP galactose 4 epimerase antibody; UDP galactose 4' epimerase antibody; UDP glucose 4 epimerase antibody; UDP-galactose 4-epimerase antibody; UDP-glucose 4-epimerase antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human UDP-glucose 4-epimerase protein (1-348AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Overexpression of UDP-Glucose 4-Epimerase Is Associated with Differentiation Grade of Gastric Cancer. de Souza M F D, et al,Disease Markers,2019

Sample type: Gastric adenocarcinoma
Sample species: Human
Antibody dilution factor: 1:100
Application: IHC
Review: Histological types of gastric adenocarcinoma evidenced by GALE immunohistochemistry. Tubular region (a), papillary region (b), poorly differentiated region (c), and delimited mucin region (d). Scale bar: 100μm.

Target Data

Function Catalyzes two distinct but analogous reactions
Gene References into Functions
  1. Mutation in UDP-galactose-4'-epimerase gene is associated with UDP-galactose-4'-epimerase deficiency. PMID: 26565537
  2. Data show the protein structure of GALE and its substrate binding and specificity. It is mutated in type III galactosemia. [review] PMID: 26162744
  3. human UDP-galactose 4'-epimerase stability is increased by variants associated with type III galactosemia but decreased by substrate and cofactor binding PMID: 25150110
  4. These data indicated a critical role of GALE in maintaining cartilage homeostasis, and suggested that GALE inhibition might contribute to OA progress. PMID: 25201731
  5. GALE variants can be arranged into three groups depending on the severity of enzyme impairment. PMID: 23644136
  6. P.K161N-hGALE causes its effects by abolishing an important interaction between the protein and the cofactor. PMID: 22613355
  7. study of hGALE crystal structure and demonstration that residue 307 acts as a gatekeeper mediating substrate access to the hGALE active site PMID: 15175331
  8. Resulst describe the relationship among UDP-galactose 4'-epimerase activity, substrate specificity, metabolic balance, and galactose sensitivity in mammalian cells. PMID: 15701638
  9. Data suggest that reduced catalytic efficiency and increased proteolytic susceptibility of UDP-galactose 4-epimerase are causative factors in type III galactosemia. PMID: 16302980
  10. Subtle biochemical and metabolic abnormalities detected in patients expressing these GALE alleles likely reflect, at least in part, the reduced enzymatic activity of the encoded GALE proteins. PMID: 18188677
  11. Our observations show that altered protein stability is due to misfolding and that loss or reduction of enzyme activity is responsible for the molecular defects underlying GALE-deficiency galactosemia. PMID: 19250319
  12. Disease-causing mutations result in a variety of changes to the steady-state parameters. Mostly these are changes in turnover number, kcat. The ability to dimerize is not affected, but some mutants have increased sensitivity to protease digestion. PMID: 16302980

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Involvement in disease Epimerase-deficiency galactosemia (EDG)
Protein Families NAD(P)-dependent epimerase/dehydratase family
Database Links

HGNC: 4116

OMIM: 230350

KEGG: hsa:2582

STRING: 9606.ENSP00000363621

UniGene: Hs.632380

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