GALE Antibody

Datasheet

Citations (1)

Code	CSB-PA613499HA01HU
Size	US$166
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Image	Western blot All lanes: GALE antibody at 2µg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 31 kDa Observed band size: 39 kDa Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA613499HA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA613499HA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA613499HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GALE Polyclonal antibody

Uniprot No.

Q14376

Target Names

GALE

Alternative Names

FLJ95174 antibody; FLJ97302 antibody; Galactose 4 epimerase UDP antibody; Galactowaldenase antibody; galE antibody; GALE_HUMAN antibody; OTTHUMP00000002991 antibody; OTTHUMP00000002994 antibody; OTTHUMP00000037931 antibody; OTTHUMP00000044857 antibody; SDR1E1 antibody; short chain dehydrogenase/reductase family 1E member 1 antibody; UDP galactose 4 epimerase antibody; UDP galactose 4' epimerase antibody; UDP glucose 4 epimerase antibody; UDP-galactose 4-epimerase antibody; UDP-glucose 4-epimerase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human UDP-glucose 4-epimerase protein (1-348AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GALE Antibody (Product code: CSB-PA613499HA01HU) is Non-conjugated. For GALE Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA613499HB01HU	GALE Antibody, HRP conjugated	ELISA
FITC	CSB-PA613499HC01HU	GALE Antibody, FITC conjugated
Biotin	CSB-PA613499HD01HU	GALE Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Overexpression of UDP-Glucose 4-Epimerase Is Associated with Differentiation Grade of Gastric Cancer de Souza M F D, et al,Disease Markers,2019

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : IHC

Sample type: Human Gastric adenocarcinoma

Sample dilution: 1:100

Review: Histological types of gastric adenocarcinoma evidenced by GALE immunohistochemistry. Tubular region (a), papillary region (b), poorly differentiated region (c), and delimited mucin region (d). Scale bar: 100μm.

By Anonymous

Target Background

Function

Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

Gene References into Functions

Mutation in UDP-galactose-4'-epimerase gene is associated with UDP-galactose-4'-epimerase deficiency. PMID: 26565537
Data show the protein structure of GALE and its substrate binding and specificity. It is mutated in type III galactosemia. [review] PMID: 26162744
human UDP-galactose 4'-epimerase stability is increased by variants associated with type III galactosemia but decreased by substrate and cofactor binding PMID: 25150110
These data indicated a critical role of GALE in maintaining cartilage homeostasis, and suggested that GALE inhibition might contribute to OA progress. PMID: 25201731
GALE variants can be arranged into three groups depending on the severity of enzyme impairment. PMID: 23644136
P.K161N-hGALE causes its effects by abolishing an important interaction between the protein and the cofactor. PMID: 22613355
study of hGALE crystal structure and demonstration that residue 307 acts as a gatekeeper mediating substrate access to the hGALE active site PMID: 15175331
Resulst describe the relationship among UDP-galactose 4'-epimerase activity, substrate specificity, metabolic balance, and galactose sensitivity in mammalian cells. PMID: 15701638
Data suggest that reduced catalytic efficiency and increased proteolytic susceptibility of UDP-galactose 4-epimerase are causative factors in type III galactosemia. PMID: 16302980
Subtle biochemical and metabolic abnormalities detected in patients expressing these GALE alleles likely reflect, at least in part, the reduced enzymatic activity of the encoded GALE proteins. PMID: 18188677
Our observations show that altered protein stability is due to misfolding and that loss or reduction of enzyme activity is responsible for the molecular defects underlying GALE-deficiency galactosemia. PMID: 19250319
Disease-causing mutations result in a variety of changes to the steady-state parameters. Mostly these are changes in turnover number, kcat. The ability to dimerize is not affected, but some mutants have increased sensitivity to protease digestion. PMID: 16302980

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Involvement in disease

Epimerase-deficiency galactosemia (EDG)

Protein Families

NAD(P)-dependent epimerase/dehydratase family

Database Links

HGNC: 4116

OMIM: 230350

KEGG: hsa:2582

STRING: 9606.ENSP00000363621

UniGene: Hs.632380

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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