Phospho-PRKCB (Thr641) Antibody

Code CSB-PA206081
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  • Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCβ (phospho-Thr641) antibody.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody.
  • Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody.
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PRKCB Polyclonal antibody
Uniprot No. P05771
Target Names PRKCB
Alternative Names KPCB_HUMAN antibody; PKC Beta antibody; PKC-B antibody; PKC-beta antibody; PKCB antibody; Prkcb antibody; PRKCB II antibody; PRKCB2 antibody; Protein kinase C beta antibody; Protein kinase C beta type antibody
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from Human PKCb
Immunogen Species Homo sapiens (Human)
Clonality Polyclonal
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration It differs from different batches. Please contact us to confirm it.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications ELISA,WB,IHC,IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
IHC 1:50-1:100
IF 1:100-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
Gene References into Functions
  1. Tumor suppressor activity of protein kinase C-beta.[review] PMID: 28571764
  2. The results demonstrate a direct relationship between SP1 binding and protein kinase CbetaII (PKCbetaII) transcription, and further suggest that this transcription factor is a contributor to the pathobiology of chronic lymphocytic leukaemia and potentially other malignant cells where PKCbetaII is overexpressed. PMID: 28233872
  3. An exaggerated vasoconstriction response to dexmedetomidine, an alpha-2 adrenergic agonist, has been associated with SNP rs9922316 in the gene for protein kinase C type beta ( PRKCB). PMID: 28482761
  4. PKC beta would sensitize cervical cancer cells to chemotherapy via reducing the chemotherapy induced autophagy in cancer cells. PMID: 28246354
  5. Loss of PRKCB2 expression is associated with colorectal cancer. PMID: 26989024
  6. significantly different gene expressions of BECN1 and PRKCB between the control and the Alzheimer's disease (AD) groups and of CDKN2A between the control and the preclinical AD groups, are reported. PMID: 26510741
  7. a primary functional variant of PRKCB (rs35015313) was identified by genotype imputation using a phased panel of 1,070 Japanese individuals from a prospective, general population cohort study and subsequent in vitro functional analyses. These results may lead to improved understanding of the disease pathways involved in primary biliary cholangitis. PMID: 28062665
  8. our findings identify PRKCB gene as a novel candidate gene for familial Meniere's Disease (MD )and its expression gradient in supporting cells of the organ of Corti deserves attention, given the role of supporting cells in K(+ )recycling within the endolymph, and its apical turn location may explain the onset of hearing loss at low frequencies in MD PMID: 27329761
  9. Activation of the Pro-Oxidant PKCbetaII-p66Shc Signaling Pathway Contributes to Pericyte Dysfunction in Skeletal Muscles of Patients With Diabetes With Critical Limb Ischemia PMID: 27600065
  10. Taken together, these data argue for a complex mechanism of PKC-beta-dependent regulation of SHCA (p66) activation involving Ser(139) and a motif surrounding Ser(213). PMID: 27624939
  11. The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
  12. Bone marrow stroma-induced resistance of chronic lymphocytic leukemia cells to arsenic trioxide involves Mcl-1 upregulation and is overcome by inhibiting the PI3Kdelta or PKCbeta signaling pathways. PMID: 26540567
  13. PPAR-delta and NKIRAS1 are downstream mediators in the PRKCB pathway in human umbilical vein endothelial cells. PMID: 26459836
  14. Lower hydrogen sulfide is associated with cardiovascular mortality, which involves PKCBII/Akt pathway in chronic hemodialysis patients. PMID: 26439941
  15. Study found a significant decrease of PRCKB1 mRNA expression in subsyndromal symptomatic depression, suggesting PRKCB1 might be a candidate gene and biomarker PMID: 26343587
  16. PKCbetaII inhibits the ubiquitination of beta-arrestin2 in an autophosphorylation-dependent manner PMID: 26545496
  17. Ionizing radiation-induced eNOS activation in human vascular endothelial cells is attributed to both the up-regulation of PKC-betaII and the increase in ROS generation which were independent of each other. PMID: 25869503
  18. Effect of PKC-beta Signaling Pathway on Expression of MCP-1 and VCAM-1 in Different Cell Models in Response to Advanced Glycation End Products PMID: 26008233
  19. Direct interaction between the two proteins leads to Apoptin-induced activation of PKC and consequently activated PKCbetaI mediates phosphorylation of Apoptin to promote its tumour-specific nuclear translocation and cytotoxic function. PMID: 25828882
  20. Gene fusions involving PRKC genes occur in several morphological and clinical subsets of benign fibrous histiocytoma, but they seem to account for only a minority of the cases. PMID: 26121314
  21. PMA primed PBTLs for polarization under flow, with protein kinase C (PKC)-delta enriched in the leading edge, PKC-betaI in the microtubule organizing center, and PKC-betaII in the uropod and peripheral region. PMID: 25548371
  22. Our data indicate a new direction for LOX-1 regulation by the modulation of the PKCbeta/NAPDH oxidase/SIRT1/HSF1 mechanism PMID: 25982096
  23. Hyperoxia can increase the expression of PKCbeta in alveolar epithelial cells and production of mitochondrial reactive oxygen species and decrease mitochondrial membrane potential. PMID: 25815500
  24. human AKAP79-anchored PKC selectively phosphorylates the Robo3.1 receptor subtype on serine 1330 PMID: 25882844
  25. that the high-concentration glucose-induced disruption of endothelial adherens junctions is mediated by tyrosine phosphorylation of vascular endothelial cadherin through PKC-beta and myosin light chain phosphorylation PMID: 25927959
  26. PKCbeta2 inhibition protects mice from gut ischemia-reperfusion injury by suppressing the adaptor p66(Shc)-mediated oxidative stress and subsequent apoptosis. PMID: 24722289
  27. The detected PDPN-PRKCB, CD63-PRKCD and LAMTOR1-PRKCD gene fusions are all predicted to result in chimeric proteins consisting of the membrane-binding part of PDPN, CD63 or LAMTOR1 PMID: 24721208
  28. PRKCB2 is specifically required for mTORC2-dependent AC9 activation and back retraction during neutrophil chemotaxis. PMID: 24600048
  29. Pharmacological inhibition PRKCB1 via their specific inhibitors and neutralisation of O2 (*-) by a cell-permeable superoxide dismutase mimetic. PMID: 24936444
  30. isoform betaII plays a central role in the PKC-dependent regulation of Kv1.5/Kvbeta1.2 channels. PMID: 24682423
  31. shRNA knockdown of PKCbeta reduced cellular proliferation, colony formation, and migratory capacity of melanoma cells and also reduced lung colonization of stably transduced melanoma cells in mice. PMID: 24406113
  32. STAT3 bound to previously undescribed negative regulatory elements within the promoter of PRKCB, which encodes PKCbetaII. PMID: 24550541
  33. Protein kinase C beta is important for the regulation of NHE-1 activity, which is associated with ERK1/2-p90RSK signaling pathways as a kinase of NHE-1 in cortical neuronal cells exposed to glutamate. PMID: 24378530
  34. Vimentin is a phosphorylation target of PKC-beta in MCP-1-treated monocytes and that PKC-beta phosphorylation is essential for vimentin secretion. PMID: 23974215
  35. CD40L may contribute to atherogenesis via PRKCB by activating endothelial cells and recruiting monocytes to them. PMID: 24039784
  36. established an important role for PKCbeta1 in PANC1 cells suggesting it would act as a suppressor of tumorigenic behavior in pancreatic cancer PMID: 23695799
  37. Data suggest that hyperglycemia promotes cerebral-barrier dysfunction through activation of PKCbeta and consequent stimulation of oxidative stress and tight junction dissolution. PMID: 23617822
  38. This review summarizes the current knowledge of both PKCbeta and PKCdelta isoforms during atherogenesis, and addresses differential roles and disputable observations of PKC isoforms. PMID: 24440741
  39. The translocation of PKCBetaII was induced by soluble amyloid Beta precursor protein alpha. PMID: 23905995
  40. Normalization of glucose levels and silencing PKCB activity neutralized the effects of hyperglycemia on occludin and RhoA/Rho-kinase/MLC2 expression, localization, and activity and consequently improved in vitro blood-brain barrier integrity and function. PMID: 23963366
  41. HIF1alpha and PKCbeta have roles in mediating the effect of oxygen and glucose during wound healing PMID: 23562913
  42. PKCbeta is the isoform responsible for Syk negative regulation PMID: 23960082
  43. PKCalpha and PKCbeta cooperate in IL-2 transcriptional transactivation in primary mouse T cells independently of the actions of PKCtheta;. PMID: 23439007
  44. Protein kinase Cbeta critically regulates dopamine D2 autoreceptor-activated dopamine transporter trafficking. PMID: 23458603
  45. Genetic variation in protein kinase C type beta may contribute toward the interindividual variation in DHV constriction responses to alpha2-AR activation by the agonist dexmedetomidine PMID: 23337848
  46. PKCbeta plays an important role in Acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin lymphoma survival. PMID: 21997316
  47. Inhibition of PKCbeta enhanced apoptosis of human autoreactive B cells. PMID: 23280626
  48. The expression of protein kinase C (PKC)-betaII and the subsequent activation of NF-kappaB in bone marrow stromal cells are prerequisites to support the survival of malignant B cells. PMID: 23328482
  49. Comp of gene expression between PROX1-overexpressing and mock-transfected cells revealed that the expression of PRKCB2 is down-regulated in PROX1-overexpressing cells. A PRKCB inhibitor suppressed growth of control cells more than PROX1-expressing cells. PMID: 22833470
  50. Estrogen receptor-alpha, RBCK1, and protein kinase C beta 1 cooperate to regulate estrogen receptor-alpha gene expression. PMID: 23042805

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Subcellular Location Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
Database Links

HGNC: 9395

OMIM: 176970

KEGG: hsa:5579

STRING: 9606.ENSP00000305355

UniGene: Hs.460355

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