Product Details

Uniprot No.

Target Names

Alternative Names

KPCB_HUMAN antibody; PKC Beta antibody; PKC-B antibody; PKC-beta antibody; PKCB antibody; Prkcb antibody; PRKCB II antibody; PRKCB2 antibody; Protein kinase C beta antibody; Protein kinase C beta type antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PRKCB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Protein kinase C beta (PRKCB) serves as a critical signaling hub in cellular processes ranging from B cell activation and immune responses to neuronal function and metabolic regulation. As a calcium-dependent serine/threonine kinase, PRKCB transduces signals from numerous receptor pathways, making it a compelling target for researchers investigating signal transduction, autoimmunity, diabetes complications, and certain malignancies where aberrant PKC signaling contributes to disease progression.

This recombinant monoclonal antibody, clone 14E3, offers the reproducibility that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human PRKCB, with the antibody raised against a synthetic peptide immunogen and purified by affinity chromatography for optimal performance.

Validation by flow cytometry demonstrates reliable intracellular detection of PRKCB in K562 cells, a human erythroleukemia line commonly used in signal transduction research. The clear separation between specific staining and isotype control confirms robust target recognition, with recommended dilutions of 1:50 to 1:200 providing flexibility to optimize signal-to-background ratios for your specific experimental conditions. The antibody is also validated for ELISA applications, supporting quantitative detection approaches.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this unconjugated format allows pairing with your preferred secondary detection system. Whether investigating PKC-mediated signaling cascades, characterizing immune cell populations, or exploring PRKCB's role in disease models, this antibody provides the consistency essential for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Gene References into Functions

Tumor suppressor activity of protein kinase C-beta.[review] PMID: 28571764
The results demonstrate a direct relationship between SP1 binding and protein kinase CbetaII (PKCbetaII) transcription, and further suggest that this transcription factor is a contributor to the pathobiology of chronic lymphocytic leukaemia and potentially other malignant cells where PKCbetaII is overexpressed. PMID: 28233872
An exaggerated vasoconstriction response to dexmedetomidine, an alpha-2 adrenergic agonist, has been associated with SNP rs9922316 in the gene for protein kinase C type beta ( PRKCB). PMID: 28482761
PKC beta would sensitize cervical cancer cells to chemotherapy via reducing the chemotherapy induced autophagy in cancer cells. PMID: 28246354
Loss of PRKCB2 expression is associated with colorectal cancer. PMID: 26989024
significantly different gene expressions of BECN1 and PRKCB between the control and the Alzheimer's disease (AD) groups and of CDKN2A between the control and the preclinical AD groups, are reported. PMID: 26510741
a primary functional variant of PRKCB (rs35015313) was identified by genotype imputation using a phased panel of 1,070 Japanese individuals from a prospective, general population cohort study and subsequent in vitro functional analyses. These results may lead to improved understanding of the disease pathways involved in primary biliary cholangitis. PMID: 28062665
our findings identify PRKCB gene as a novel candidate gene for familial Meniere's Disease (MD )and its expression gradient in supporting cells of the organ of Corti deserves attention, given the role of supporting cells in K(+ )recycling within the endolymph, and its apical turn location may explain the onset of hearing loss at low frequencies in MD PMID: 27329761
Activation of the Pro-Oxidant PKCbetaII-p66Shc Signaling Pathway Contributes to Pericyte Dysfunction in Skeletal Muscles of Patients With Diabetes With Critical Limb Ischemia PMID: 27600065
Taken together, these data argue for a complex mechanism of PKC-beta-dependent regulation of SHCA (p66) activation involving Ser(139) and a motif surrounding Ser(213). PMID: 27624939
The study aimed to identify a small set of genetic signatures that may reliably predict the individuals with a high genetic propensity to heroin addiction. A set of 4 genes (JUN, CEBPB, PRKCB, ENO2, or CEBPG) could predict the diagnosis of heroin addiction with the accuracy rate around 85% in our dataset. PMID: 27495086
Bone marrow stroma-induced resistance of chronic lymphocytic leukemia cells to arsenic trioxide involves Mcl-1 upregulation and is overcome by inhibiting the PI3Kdelta or PKCbeta signaling pathways. PMID: 26540567
PPAR-delta and NKIRAS1 are downstream mediators in the PRKCB pathway in human umbilical vein endothelial cells. PMID: 26459836
Lower hydrogen sulfide is associated with cardiovascular mortality, which involves PKCBII/Akt pathway in chronic hemodialysis patients. PMID: 26439941
Study found a significant decrease of PRCKB1 mRNA expression in subsyndromal symptomatic depression, suggesting PRKCB1 might be a candidate gene and biomarker PMID: 26343587
PKCbetaII inhibits the ubiquitination of beta-arrestin2 in an autophosphorylation-dependent manner PMID: 26545496
Ionizing radiation-induced eNOS activation in human vascular endothelial cells is attributed to both the up-regulation of PKC-betaII and the increase in ROS generation which were independent of each other. PMID: 25869503
Effect of PKC-beta Signaling Pathway on Expression of MCP-1 and VCAM-1 in Different Cell Models in Response to Advanced Glycation End Products PMID: 26008233
Direct interaction between the two proteins leads to Apoptin-induced activation of PKC and consequently activated PKCbetaI mediates phosphorylation of Apoptin to promote its tumour-specific nuclear translocation and cytotoxic function. PMID: 25828882
Gene fusions involving PRKC genes occur in several morphological and clinical subsets of benign fibrous histiocytoma, but they seem to account for only a minority of the cases. PMID: 26121314
PMA primed PBTLs for polarization under flow, with protein kinase C (PKC)-delta enriched in the leading edge, PKC-betaI in the microtubule organizing center, and PKC-betaII in the uropod and peripheral region. PMID: 25548371
Our data indicate a new direction for LOX-1 regulation by the modulation of the PKCbeta/NAPDH oxidase/SIRT1/HSF1 mechanism PMID: 25982096
Hyperoxia can increase the expression of PKCbeta in alveolar epithelial cells and production of mitochondrial reactive oxygen species and decrease mitochondrial membrane potential. PMID: 25815500
human AKAP79-anchored PKC selectively phosphorylates the Robo3.1 receptor subtype on serine 1330 PMID: 25882844
that the high-concentration glucose-induced disruption of endothelial adherens junctions is mediated by tyrosine phosphorylation of vascular endothelial cadherin through PKC-beta and myosin light chain phosphorylation PMID: 25927959
PKCbeta2 inhibition protects mice from gut ischemia-reperfusion injury by suppressing the adaptor p66(Shc)-mediated oxidative stress and subsequent apoptosis. PMID: 24722289
The detected PDPN-PRKCB, CD63-PRKCD and LAMTOR1-PRKCD gene fusions are all predicted to result in chimeric proteins consisting of the membrane-binding part of PDPN, CD63 or LAMTOR1 PMID: 24721208
PRKCB2 is specifically required for mTORC2-dependent AC9 activation and back retraction during neutrophil chemotaxis. PMID: 24600048
Pharmacological inhibition PRKCB1 via their specific inhibitors and neutralisation of O2 (*-) by a cell-permeable superoxide dismutase mimetic. PMID: 24936444
isoform betaII plays a central role in the PKC-dependent regulation of Kv1.5/Kvbeta1.2 channels. PMID: 24682423
shRNA knockdown of PKCbeta reduced cellular proliferation, colony formation, and migratory capacity of melanoma cells and also reduced lung colonization of stably transduced melanoma cells in mice. PMID: 24406113
STAT3 bound to previously undescribed negative regulatory elements within the promoter of PRKCB, which encodes PKCbetaII. PMID: 24550541
Protein kinase C beta is important for the regulation of NHE-1 activity, which is associated with ERK1/2-p90RSK signaling pathways as a kinase of NHE-1 in cortical neuronal cells exposed to glutamate. PMID: 24378530
Vimentin is a phosphorylation target of PKC-beta in MCP-1-treated monocytes and that PKC-beta phosphorylation is essential for vimentin secretion. PMID: 23974215
CD40L may contribute to atherogenesis via PRKCB by activating endothelial cells and recruiting monocytes to them. PMID: 24039784
established an important role for PKCbeta1 in PANC1 cells suggesting it would act as a suppressor of tumorigenic behavior in pancreatic cancer PMID: 23695799
Data suggest that hyperglycemia promotes cerebral-barrier dysfunction through activation of PKCbeta and consequent stimulation of oxidative stress and tight junction dissolution. PMID: 23617822
This review summarizes the current knowledge of both PKCbeta and PKCdelta isoforms during atherogenesis, and addresses differential roles and disputable observations of PKC isoforms. PMID: 24440741
The translocation of PKCBetaII was induced by soluble amyloid Beta precursor protein alpha. PMID: 23905995
Normalization of glucose levels and silencing PKCB activity neutralized the effects of hyperglycemia on occludin and RhoA/Rho-kinase/MLC2 expression, localization, and activity and consequently improved in vitro blood-brain barrier integrity and function. PMID: 23963366
HIF1alpha and PKCbeta have roles in mediating the effect of oxygen and glucose during wound healing PMID: 23562913
PKCbeta is the isoform responsible for Syk negative regulation PMID: 23960082
PKCalpha and PKCbeta cooperate in IL-2 transcriptional transactivation in primary mouse T cells independently of the actions of PKCtheta;. PMID: 23439007
Protein kinase Cbeta critically regulates dopamine D2 autoreceptor-activated dopamine transporter trafficking. PMID: 23458603
Genetic variation in protein kinase C type beta may contribute toward the interindividual variation in DHV constriction responses to alpha2-AR activation by the agonist dexmedetomidine PMID: 23337848
PKCbeta plays an important role in Acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin lymphoma survival. PMID: 21997316
Inhibition of PKCbeta enhanced apoptosis of human autoreactive B cells. PMID: 23280626
The expression of protein kinase C (PKC)-betaII and the subsequent activation of NF-kappaB in bone marrow stromal cells are prerequisites to support the survival of malignant B cells. PMID: 23328482
Comp of gene expression between PROX1-overexpressing and mock-transfected cells revealed that the expression of PRKCB2 is down-regulated in PROX1-overexpressing cells. A PRKCB inhibitor suppressed growth of control cells more than PROX1-expressing cells. PMID: 22833470
Estrogen receptor-alpha, RBCK1, and protein kinase C beta 1 cooperate to regulate estrogen receptor-alpha gene expression. PMID: 23042805

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Subcellular Location

Cytoplasm. Nucleus. Membrane; Peripheral membrane protein.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Database Links

HGNC: 9395

OMIM: 176970

KEGG: hsa:5579

STRING: 9606.ENSP00000305355

UniGene: Hs.460355

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Code	CSB-RA074719A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing K562 cells stained with CSB-RA074719A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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PRKCB Recombinant Monoclonal Antibody

Product Details

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Target Background