Recombinant Apis mellifera Vitellogenin (Vg), partial

Code CSB-YP768142DNK
MSDS
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Source Yeast
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Code CSB-EP768142DNK-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768142DNK
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP768142DNK
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vg
Uniprot No.
Alternative Names
Vg; Vitellogenin
Species
Apis mellifera (Honeybee)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Precursor of the egg-yolk proteins that are sources of nutrients during embryonic development. Involved in the differentiation of honeybee larvae into queens.
Gene References into Functions
  1. A tight coregulatory relationship exists between Vg and juvenile hormone (JH) in the regulation of honeybee behavioral maturation that is manifest at the genomic level; acting through common pathways, they regulate brain gene expression and behavior. PMID: 23889463
  2. The study demonstrates a causal link between the Vg knockdown forager phenotype and variation in the abundance of microRNAs in different tissues, with possible consequences for the regulation of foraging behavior. PMID: 23788711
  3. spectroscopic results strengthen our model of the Vg polyserine tract as a flexible domain linker shielded by phosphorylation PMID: 22573762
  4. identify vitellogenin protein in the hypopharyngeal glands (brood food producing head glands) of nursing worker bees and in adjacent head fat body cells for the first time PMID: 20337562
  5. Data show that silencing of vitellogenin expression causes a significant increase in juvenile hormone titer and its putative receptor. PMID: 16122739
  6. Vitellogenin protein, which is synthesized at high levels in honey bee queens and is abundant in long-lived workers, protects worker bees from oxidative stress PMID: 16418279
  7. In precocious foragers, downregulation of vitellogenin gene activity apparently has little effect on the juvenile hormone titer. PMID: 18545981
  8. Workers in the queenless colonies with active ovaries that have an upregulation of vit transcript expression levels, and thus assumed vitellogenin protein levels. PMID: 19096928
  9. Bacteria- or water-injection induce an equivalent and strong depression in the levels of Vg protein, which was corroborated in M. luteus-injected bees, but not in S. marcescens-injected ones, by a statistically significant decrease in vg mRNA levels. PMID: 19309002

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Subcellular Location
Secreted.
Tissue Specificity
Accumulates in the hemolymph. Represents up to 70% of the queen's hemolymph proteins. During the first week of the worker adult life, when it becomes a nurse bee and performs brood-rearing tasks, the vitellogenin titer increases and may account for up to
Database Links

KEGG: ame:406088

UniGene: Ame.206

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