The expression of recombinant Macaca fascicularis IL4 protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the yeast, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 25-153aa of the Macaca fascicularis IL4 protein. This N-terminal 6xHis-tagged recombinant Macaca fascicularis IL4 protein is also characterized by high purity, >90%. Under SDS-PAGE condition, this recombinant IL4 protein migrated to the bands of about 30 kDa and 17 kDa molecular weight, corresponding to the glycosylated and deglycosylated forms, respectively.
As the B cell-stimulatory factor-1, IL-4 is mainly produced by activated T cells. In T-cells, IL-4 induces the differentiation of naïve CD4 T cells into Th2 cells, in B cells, IL-4 drives the immunoglobulin (Ig) class switch to IgG1 and IgE, and in macrophages, IL-4 and IL-13 induce alternative macrophage activation. It has been well-documented that it plays a major role in hormone control and adaptive immunity. In addition, IL-4 a key regulator in inflammation response and wound repair. Many papers give insights into the functional formation of IL-4. Studies found that IL-4 coordinates the Th2-type immune response in inflammatory diseases such as asthma. IL-27 can inhibit the development of both Th2 and Th1. Prolonged residence of an albumin–IL-4-fusion protein in secondary lymphoid organs ameliorates experimental autoimmune encephalomyelitis. IL-4 absence could trigger the distinct pathways in apical periodontitis development. Association between IL-4 gene polymorphisms and IL-4 serum levels in patients with allergic rhinitis. Status of IL‐4 and IL‐10 driven markers in experimental models of visceral leishmaniasis.
||Greater than 90% as determined by SDS-PAGE.
||IL4Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
||Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
||Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| Tris-based buffer,50% glycerol
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.