Recombinant Dog Nuclear factor NF-kappa-B p105 subunit(NFKB1)

Code CSB-YP739094DO
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Source Yeast
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Code CSB-EP739094DO
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Source E.coli
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Code CSB-EP739094DO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP739094DO
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Source Baculovirus
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Code CSB-MP739094DO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NFKB1
Uniprot No. Q6F3J0
Alternative Names NFKB1; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) [Cleaved into: Nuclear factor NF-kappa-B p50 subunit]
Species Canis lupus familiaris (Dog) (Canis familiaris)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105 (By similarity).
Gene References into Functions
  1. These findings suggest that stimulation of MEK/ERK signaling pathway by NF-kappaB activation regulates IL-1beta-induced COX-2 expression and subsequent prostaglandin E2 release in canine dermal fibroblasts. PMID: 26549149
  2. The dog p65 and cat p65 subunits of NF-kappaB complementary DNA encoded 542 and 555 amino acids, respectively. The p65 messenger RNA is highly expressed in the dog stomach and cat heart and adipose tissue. PMID: 25770673
  3. Non-cytotoxic levels of propofol pretreatment can down-regulate LPS-induced inflammatory responses in canine PBMCs, possibly by inhibiting the nuclear translocation of the NF-kappaB p65 protein. PMID: 25312048
  4. These results suggest that NFkappaB and CAMs may play important roles in the pathogenesis of canine lymphocytic-plasmacytic enteritis. PMID: 23786670
  5. The results indicated that activation of NF-kappaB could contribute to the proliferation of neoplastic lymphoid cells, and bortezomib would have suppressive effects on the NF-kappaB activation and the proliferation of neoplastic lymphoid cells in dogs. PMID: 23337362
  6. These data suggest that NOD2 and NFkappaB play an important role in the pathogenesis of LPC. PMID: 23600687
  7. IGF-1 and PDGF-bb are potent inhibitors of IL-1beta-mediated activation of NF-kappaB and apoptosis in chondrocytes, may be mediated in part through suppression of Src/PI-3K/AKT pathway. PMID: 22194879

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Subcellular Location Nucleus, Cytoplasm
Database Links

KEGG: cfa:442859

STRING: 9615.ENSCAFP00000015815

UniGene: Cfa.10766

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