Product Details

Uniprot No.

Target Names

Alternative Names

Neurogenic locus notch homolog protein 1 (Notch 1) (hN1) (Translocation-associated notch protein TAN-1) [Cleaved into: Notch 1 extracellular truncation (NEXT), Notch 1 intracellular domain (NICD)], NOTCH1, TAN1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Notch1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NOTCH1 serves as a critical transmembrane receptor in the evolutionarily conserved Notch signaling pathway, governing fundamental processes including cell fate determination, stem cell maintenance, and tissue homeostasis. Upon ligand binding, NOTCH1 undergoes sequential proteolytic cleavages that release the intracellular domain (NICD), which translocates to the nucleus to regulate transcription of target genes. Dysregulation of this pathway has been implicated in numerous developmental disorders and malignancies, making NOTCH1 a compelling target for researchers investigating cardiovascular development, neurogenesis, and cancer biology.

This recombinant monoclonal antibody, clone 4F6, offers the reproducibility and consistency that demanding experimental workflows require. Generated against a synthetic peptide derived from human NOTCH1 and produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. The affinity-chromatography purification ensures high purity suitable for sensitive detection methods.

Validation studies demonstrate robust performance across multiple applications. Western blot analysis at 1:1000 dilution successfully detects NOTCH1 in HepG2, THP-1, and HL-60 whole cell lysates, with an observed band at approximately 120 kDa rather than the predicted 273 kDa full-length protein. This molecular weight corresponds to processed NOTCH1 fragments, consistent with the receptor's characteristic proteolytic activation and the generation of cleaved forms such as NEXT or NICD. Immunohistochemical staining has been validated in paraffin-embedded human brain tissue at 1:100 dilution using citrate buffer antigen retrieval, confirming utility for tissue-based investigations.

Researchers studying epigenetic regulation, neural development, cardiovascular biology, or stem cell differentiation will find this antibody a reliable tool for interrogating NOTCH1 expression and processing in human samples.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

Gene References into Functions

beta1,4GalTV stimulates transdifferentiation of glioma stem-like cells into endothelial cells by activating Notch1 signaling PMID: 29269413
Expression of translocation-associated notch protein (Notch1) is elevated in patients with chronic hepatitis C (HCV). Inhibition of Notch signaling downregulates HCV-specific Th22 cells and interleukin-22 production, which is accompanied by the reduction of aryl hydrocarbon receptor and modulatory cytokines (IL-6 and tumor necrosis factor-alpha). PMID: 28410452
MiR-497 approximately 195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch1 and HIF1A activity. PMID: 28685750
The results demonstrated that treatment with norepinephrine(NE) enhanced cell viability and invasion, and inhibited apoptosis of Pancreatic ductal adenocarcinoma (PDAC) cells; however, these effects were suppressed following treatment with Notch1specific siRNA and DAPT. In conclusion, NE may enhance the malignant biological behaviors of PDAC via activating the Notch1 pathway. PMID: 30226612
sinonasal inverted papillomas lesions presented reduced Notch-1 expression compared to nasal polyposis. Dysplastic lesions presented low Notch-1 immunopositivity. Enhancement of Notch-1 gene expression was also associated with inflammation. PMID: 30297114
NOTCH signalling can modulate chromatin structure autonomously and non-autonomously. PMID: 29743479
Notch1 activation and epithelial-mesenchymal transition are coupled to promote squamous cell carcinomas tumor initiation in concert with transforming growth factor (TGF)-beta present in the tumor microenvironment. PMID: 29170450
the reported Dowling-Degos mutations of POFUT1, except for M262T, fail to rescue Notch1 signaling efficiently in the CRISPR-engineered POFUT1-/- background; these studies identify POFUT1 as a potential target for cancers driven by Notch1 mutations and provide a structural roadmap for its inhibition PMID: 28334865
Study reports that IL-17 stimulation induces NOTCH1 activation in oligodendrocyte progenitor cells, contributing to Th17-mediated demyelinating disease. PMID: 28561022
High NOTCH1 expression is associated with endometriosis. PMID: 29398419
Mir-34a regulates cigarette smoke extract-induced respiratory endothelial cell apoptosis by targeting Notch-1 protein. PMID: 29373969
shear stress was able to induce arterial endothelial differentiation of stem cells from human exfoliated deciduous teeth, and VEGF-DLL4/NotchEphrinB2 signaling was involved in this process. PMID: 30015843
Notch1 receptor intracellular domain and Ets-1 cooperatively bind to the DNA of the TRPA1 promoter to up-regulate transcription in the context of living cells. PMID: 28220825
miR-139-5p was identified as a tumour suppressor by negatively targeting Notch1, and this work suggests a possible molecular mechanism of the miR-139/Notch1/EMT axis for glioma treatment. PMID: 30170559
Data suggest that CBX4 expression is up-regulated in breast cancer and is correlated with unfavorable overall survival; CBX4 promotes cell growth and migration via transcriptionally suppressing expression of miR137 to trigger Notch1 signaling pathway. (CBX4 = chromobox homolog 4; miR137 = microRNA 137; Notch1 = neurogenic locus notch homolog protein-1) PMID: 29229426
Data indicate that stanniocalcin 1 (STC1) is a non-canonical NOTCH1 ligand and acts as a crucial regulator of stemness in glioblastoma (GBM). PMID: 29196129
showed here that 3' UTR NOTCH1 mutations are associated with low CD20 expression and with relative resistance to anti-CD20 immunotherapy in vitro PMID: 28550186
MiR92a3p blocks the progression of Wilms tumor by targeting NOTCH1. PMID: 29845267
identified a branch of the XIST/miR-137/Notch-1 pathway that regulates proliferation and TGF-beta1-induced EMT in NSCLC, which could be involved in NSCLC progression PMID: 29812958
In conclusion, we demonstrated that EV71 infection induced elevated expressions of TLR3/4 and Notch1/2 in CD14+ monocytes. PMID: 29702280
Data show that fluid shear stress activates NOTCH signaling, which upregulates GJA4 (commonly, Cx37) and downstream cell cycle inhibitor CDKN1B (p27). PMID: 29247167
EZH2 mutations coexisted with mutations of NOTCH1, IL7R, and PHF6 in the two Adult T-cell Acute Lymphoblastic Leukemia patients, and they responded poorly to chemotherapy and experienced difficult clinical histories and inferior outcomes PMID: 28747286
Crosstalk between TLR4 and Notch1 signaling regulates the inflammatory response in the IgAN and maybe plays an important role in the progression of IgAN. PMID: 29230705
These results suggest that gastric cancer progression is not associated with a unique signaling pathway and that a feedback loop may exist between the HGF/c-Met and Notch1 signaling pathways, which may result in therapeutic resistance. PMID: 29781036
Results indicated that CRNDE functioned as an oncogene in osteosarcoma cell lines, and CRNDE may exert its oncogenic role via regulating Notch1 signaling and EMT in osteosarcoma. PMID: 29246789
NOTCH1 is a central mediator of TGFbetamediated FOXP3 expression and NOTCH1 inhibition produces a significant reduction of melanoma cell proliferation and viability. PMID: 29620159
Study observed membranous Notch1 expression in 31% of the oral leukoplakia (OL) samples. Membranous Notch1 expression was significantly associated with the severity of dysplasia and development of oral squamous cell carcinoma (OSCC). Also, the extent of membranous Notch1 expression was found to increase during carcinogenesis. PMID: 29620248
High NOTCH1 expression is associated with head and neck squamous cell carcinoma. PMID: 29047105
Notch1 role in the angiomyolipoma differentiation.Rheb transcription is regulated by direct Notch1 binding to the Rheb promoter. PMID: 29184052
Notch1 signaling may contribute to the pathogenesis of PV by regulating Th17/Treg immune imbalance. PMID: 29686529
we demonstrate for the first time the presence of NOTCH1 mutation in cases of Hodgkin transformation of B-CLL and outline the clinicopathological characteristics and treatment outcomes for these patients. PMID: 27686521
Endothelial NOTCH1 is responsive to shear stress, and is necessary for the maintenance of junctional integrity, cell elongation, and suppression of proliferation, phenotypes induced by laminar shear stress. PMID: 29158473
Notch1 activation in glioma stem cells specifically induces expression of the long noncoding RNA, TUG1. PMID: 27922002
B-AP15 induced cytotoxic response to hepatocellular carcinoma cells by augmenting ER stress/UPR and inhibiting Wnt/Notch1 signaling pathways. PMID: 29454609
Notch1 is a direct target of miR-449a and positively regulated by circRNA-000911 in breast cancer cell lines. PMID: 29431182
human thymopoiesis involves complex spatiotemporal regulation of Notch ligand expression, which ensures the coordinated delivery of niche-specific NOTCH1 signals required for dynamic T-cell development. PMID: 30042180
Notch1 was confirmed as a target of miR1395p and, showed a marked downregulated expression together with its pathway downstream factors during mesenchymal stem cells osteogenesis. PMID: 29565453
High expression of NOTCH1 was associated with better overall survival in head and neck squamous cell carcinoma. PMID: 29533972
This study identifies the unique role of JAG1-induced Notch activation in the pathogenesis of multiple myeloma PMID: 29242532
Results indicate that lncRNA SNHG1 may be a potential predictor of prognosis in esophageal squamous cell cancer (ESCC) patients, and that knockdown of SNHG1 suppressed the Notch signaling pathway by reducing the Notch1 expression levels in ESCC cells. PMID: 29081407
ATRX, NOTCH1 and NOTCH2 expression varies in angiosarcomas and shows significant correlations with site of origin and poor clinical outcome PMID: 28796347
CK2 inhibitor CX-4945 destabilizes NOTCH1 and synergizes with JQ1 against human T-acute lymphoblastic leukemic cells. PMID: 27758824
Western blotting demonstrated that DUSP1 dephosphorylated pERK and PTEN dephosphorylated pAKT. Collectively, we found a link among HBx, the Notch1 pathway, DUSP1/PTEN, and ERK/AKT pathways, which influenced hepatocellular carcinoma (HCC) cell survival and could be a therapeutic target for HCC treatment. PMID: 29048612
Notch 1 was key in the progression of breast cancer, and knocking down the expression of Notch 1 significantly suppressed the proliferation and invasion of breast cancer cells. PMID: 29207146
Results provide insight into the interrelationship between T-ALL oncogenic networks and the therapeutic efficacy of dual PI3Kgamma/delta inhibition in the context of NOTCH1 and cMYC signaling. PMID: 28716817
Notch1 signaling is an essential downstream pathway of MDM2 in mediating high glucose-induced mitotic catastrophe in podocytes. PMID: 28643424
Effect of Notch1 gene on proliferation and chemo sensitivity of lung cancer A549 cells was studied. PMID: 28678318
results indicate that an ATP2C1/NOTCH1 axis might be critical for keratinocyte function and cutaneous homeostasis, suggesting a plausible model for the pathological features of Hailey-Hailey disease PMID: 27528123
MAFB enhanced leukemogenesis by the naturally occurring Notch1 mutants, decreased disease latency, and increased disease penetrance. PMID: 29138297
the tumorsuppressive roles of miR455 in modulating EOC proliferation and invasion through regulation of Notch1 expression. PMID: 29039517

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Involvement in disease

Aortic valve disease 1 (AOVD1); Adams-Oliver syndrome 5 (AOS5)

Subcellular Location

Cell membrane; Single-pass type I membrane protein.; [Notch 1 intracellular domain]: Nucleus.

Protein Families

NOTCH family

Tissue Specificity

In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Database Links

HGNC: 7881

OMIM: 109730

KEGG: hsa:4851

STRING: 9606.ENSP00000277541

UniGene: Hs.495473

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Code	CSB-RA599445A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate All lanes: Notch1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 273 kDa Observed band size: 120 kDa IHC image of CSB-RA599445A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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NOTCH1 Recombinant Monoclonal Antibody

Product Details

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Target Background