Code | CSB-RA024077A33phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
In the production of the phospho-TP53 (S33) recombinant monoclonal antibody, the initial phase entails the extraction of genes responsible for coding the TP53 antibody from rabbits previously subjected to immunization with a synthesized peptide originating from the human TP53 protein phosphorylated at S33. Subsequently, these antibody genes are introduced into specialized expression vectors. Following this step, the genetically modified vectors are meticulously transfected into host suspension cells. Once this transfection is successfully achieved, positive cells are cultivated to facilitate the robust expression and subsequent secretion of the antibodies. Following this cell culture phase, the phospho-TP53 (S33) recombinant monoclonal antibody undergoes a rigorous purification process employing affinity chromatography techniques, which effectively isolates the antibody from the surrounding cell culture supernatant. Ultimately, the antibody's efficacy is comprehensively assessed through a battery of examinations, spanning ELISA, IHC, and IF tests, conclusively affirming its ability to interact effectively with human TP53 protein phosphorylated at S33.
Phosphorylation of p53 at S33 is a critical mechanism for coordinating DNA repair, cell cycle regulation, and cell fate decisions in response to stress and damage. Dysregulation of this phosphorylation event can lead to uncontrolled cell proliferation and is often observed in cancer cells.
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