Code | CSB-RA825742A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
CUSABIO extracted B lymphocytes from an animal immunized with a synthetic peptide derived from human TP53. The B cells were then fused with myeloma cells to produce hybridomas. The variable light and variable heavy domains of TP53 antibody-producing hybridomas were sequenced to construct a vector for a recombinant generation. Subsequently, the TP53 monoclonal antibody gene-containing vector was transfected into cells for cultivation, and the TP53 recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. The specificity of the purified antibody for human TP53 protein detection was evaluated through ELISA, WB, IHC, and IFC applications.
The TP53 protein, also known as p53, is a transcription factor that maintains genomic stability and prevents the accumulation of mutations that could lead to cancer. Dysfunction or loss of TP53 is commonly associated with cancer development and progression. When a cell experiences DNA damage or other stresses that could potentially lead to cancer, the p53 protein is activated and triggers a series of responses to prevent the damaged cell from replicating and potentially becoming a tumor. These responses include cell cycle arrest, apoptosis, and DNA repair.
There are currently no reviews for this product.