Code | CSB-RA214807A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The production of the TP53 recombinant monoclonal antibody involves a complex process that begins with the collection of the TP53 monoclonal antibody and the determination of its genetic sequence through gene sequencing. The next step involves the construction of a vector containing the TP53 monoclonal antibody gene and its transfection into a host cell line for culturing. A synthesized peptide derived from human p53 (acetyl K370) is used to create the TP53 monoclonal antibody. The resulting TP53 recombinant monoclonal antibody undergoes purification using affinity chromatography to ensure its high purity. It is then tested for its specificity in ELISA and IHC assays to validate its ability to accurately detect TP53. It only detects human TP53 protein.
The TP53 protein, also known as p53, plays a critical role in maintaining genomic stability and preventing the development of cancer. It functions as a tumor suppressor by regulating the cell cycle, DNA repair, cellular stress response, senescence, and development and differentiation. TP53 regulates the cell cycle and prevents the proliferation of cells with DNA damage or other mutations that could lead to cancer. When DNA damage occurs, TP53 activates pathways that either promote DNA repair or, if repair is not possible, initiate apoptosis to eliminate the damaged cells. TP53 also plays a role in DNA repair, by activating pathways that help to correct damaged DNA. TP53 can directly bind to damaged DNA and recruit proteins that help to repair it. TP53 can also induce senescence, a state of irreversible growth arrest, in cells that have undergone too many cell divisions or have suffered DNA damage. TP53 plays a role in normal development and differentiation, by regulating the expression of genes that control cell fate and differentiation.
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