TP53, also known as Tumor Protein p53, and by other names such as CC7, Cellular tumor antigen p53, LFS1, Mutant tumor protein 53, and p53, is a transcription factor encoded by the TP53 gene located on human chromosome 17. It is a key regulator of the cell cycle. The mechanism of action for TP53 involves responding to cellular stress signals, such as DNA damage, hypoxia, and metabolic stress, by regulating the expression of multiple downstream genes, inducing cell cycle arrest, apoptosis, senescence, DNA repair, or metabolic changes.
TP53 is involved in the regulation of the cell cycle, DNA damage repair, apoptosis, and senescence, and is widely considered the "guardian of the genome." Mutations in TP53 are associated with the development of various human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Additionally, TP53 regulates cellular metabolism, ferroptosis, the tumor microenvironment, and autophagy, all of which contribute to tumor suppression. Mutations in TP53 not only impair its tumor-suppressive function but may also confer oncogenic properties to mutant p53, making it an attractive target for the development of new anticancer drugs.
Popular Product:
TP53 Recombinant Monoclonal Antibody (CSB-RA236796A0HU)
Validated Data
Western Blot
Positive WB detected in: COLO320 whole cell lysate, 293 whole cell lysate
All lanes: TP53 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
Observed band size: 53 kDa
IHC image of CSB-RA236796A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 Cells with CSB-RA236796A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The following TP53 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
TP53 Proteins
TP53 Proteins for Equus caballus (Horse)
TP53 Proteins for Bos taurus (Bovine)
TP53 Proteins for Gallus gallus (Chicken)
TP53 Proteins for Felis catus (Cat) (Felis silvestris catus)
TP53 Proteins for Ovis aries (Sheep)
TP53 Proteins for Macaca mulatta (Rhesus macaque)
TP53 Proteins for Homo sapiens (Human)
TP53 Proteins for Mus musculus (Mouse)
TP53 Proteins for Bos indicus (Zebu)
TP53 Proteins for Oryzias latipes (Japanese rice fish) (Japanese killifish)
TP53 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
TP53 Proteins for Rattus norvegicus (Rat)
TP53 Proteins for Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)
TP53 Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
TP53 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
TP53 Proteins for Macaca fuscata fuscata (Japanese macaque)
TP53 Proteins for Xenopus laevis (African clawed frog)
TP53 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
TP53 Proteins for Platichthys flesus (European flounder) (Pleuronectes flesus)
TP53 Proteins for Marmota monax (Woodchuck)
TP53 Proteins for Xiphophorus helleri (Green swordtail)
TP53 Proteins for Ictalurus punctatus (Channel catfish) (Silurus punctatus)
TP53 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
TP53 Proteins for Equus asinus (Donkey) (Equus africanus asinus)
TP53 Proteins for Spermophilus beecheyi (California ground squirrel) (Otospermophilus beecheyi)
TP53 Proteins for Xiphophorus maculatus (Southern platyfish) (Platypoecilus maculatus)
TP53 Proteins for Delphinapterus leucas (Beluga whale)
TP53 Proteins for Oryctolagus cuniculus (Rabbit)
TP53 Proteins for Tupaia belangeri (Common tree shrew) (Tupaia glis belangeri)
TP53 Proteins for Sus scrofa (Pig)
TP53 Proteins for Cavia porcellus (Guinea pig)
TP53 Proteins for Barbus barbus (Barbel) (Cyprinus barbus)
TP53 Proteins for Tetraodon miurus (Congo puffer)
