Code | CSB-RA592348A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
The synthesis of the TP53 recombinant monoclonal antibody comprises a multistep process. The initial stage involves harvesting the TP53 monoclonal antibody and analyzing its gene sequence. A vector containing the TP53 monoclonal antibody gene is then constructed and transfected into a host cell line for culturing. During the TP53 monoclonal antibody production, a synthesized peptide that is derived from human TP53 is utilized as an immunogen. The TP53 recombinant monoclonal antibody is subsequently isolated and purified through affinity chromatography from the cell culture supernatant. Finally, the specificity of the TP53 recombinant monoclonal antibody is evaluated via ELISA and IF assays. It only detects human TP53 protein.
The TP53 protein, commonly referred to as p53, is a tumor suppressor activated in response to various stress signals, including DNA damage, oxidative stress, and hypoxia, leading to the stabilization and accumulation of the protein in the nucleus. Once activated, TP53 regulates the expression of various target genes involved in cell cycle arrest, DNA repair, and apoptosis. TP53 can also inhibit the formation of new blood vessels by regulating the expression of angiogenesis-related genes. Mutations in the TP53 gene are associated with the development of various types of cancer, as they can lead to the loss or reduction of TP53 function.
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