Recombinant Mouse Transcription factor AP-1(Jun)

Code CSB-YP011972MO
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Source Yeast
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Code CSB-EP011972MO
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Source E.coli
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Code CSB-EP011972MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011972MO
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Source Baculovirus
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Code CSB-MP011972MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Jun
Uniprot No. P05627
Alternative Names Jun; Transcription factor AP-1; AH119; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; Jun A
Species Mus musculus (Mouse)
Expression Region 1-334
Target Protein Sequence MTAKMETTFY DDALNASFLQ SESGAYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVSGA GMVAPAVASV AGAGGGGGYS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPSQ PQQQQQPPQP PHHLPQQIPV QHPRLQALKE EPQTVPEMPG ETPPLSPIDM ESQERIKAER KRMRNRIAAS KCRKRKLERI ARLEEKVKTL KAQNSELAST ANMLREQVAQ LKQKVMNHVN SGCQLMLTQQ LQTF
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells (By similarity).
Gene References into Functions
  1. NOS1 inhibition prevents nuclear translocation of the AP1 transcription factor subunits. PMID: 29605772
  2. these results highlight the metabolic oversight of the nerve injury response via the regulation of JUN activity by O-GlcNAcylation, a pathway that could be important in the neuropathy associated with diabetes and aging. PMID: 30012597
  3. These results suggest JUN and DDIT3 are independently regulated pro-death signaling molecules in retinal ganglion cells and together account for the vast majority of apoptotic signaling in retinal ganglion cells after axonal injury PMID: 28969695
  4. persistent distention/stretch on colonic smooth muscle cells could suppress SCF production probably through Ca(2+) -ERK-AP-1-miR-34c deregulation. PMID: 28580775
  5. Jun is a major regulator of RGC somal degeneration after glaucomatous ocular hypertensive injury. These results suggest in glaucomatous neurodegeneration, JNK-JUN signaling has a major role as a pro-death signaling pathway between axonal injury and somal degeneration. PMID: 28726785
  6. Loss of epidermal AP1 reduces filaggrin level, alters chemokine expression and produces an ichthyosis-related phenotype. PMID: 28569792
  7. Study provide evidence that c-jun expression is regulated by WDR13. PMID: 28222755
  8. The positive feedback regulation of OCT4 and c-JUN, resulting in the continuous expression of oncogenes such as c-JUN, seems to play a critical role in the determination of the cell fate decision from induced pluripotent stem cells to cancer stem cells in liver cancer. PMID: 27341307
  9. In transgenic mice with graded elevation of Schwann cell c-Jun, high c-Jun elevation is a potential pathogenic mechanism because it inhibits myelination. There was no link between c-Jun elevation and tumorigenesis. Modest c-Jun elevation, which is beneficial for regeneration, is well tolerated during Schwann cell development and in the adult and is compatible with restoration of myelination and function after injury. PMID: 29109239
  10. Overall, our results provide the first evidence that HDAC6 is capable of inducing expression of pro-inflammatory genes by regulating the ROS-MAPK-NF-kappaB/AP-1 pathways and serves as a molecular target for inflammation. PMID: 27208785
  11. c-jun expression patterns suggest that c-jun has a pivotal role in the proliferation of embryonic neural precursor cells, but it has also other roles in adult neurogenesis PMID: 28091742
  12. Data show that miR-200b and miR-200c could directly bind the 3' UTR of JUN, and JUN activated the transcription of srebp1 to increase lipid accumulation. PMID: 27166182
  13. transgenic mice overexpressing sPLA2 -IIA keratinocytes showed enhanced activation of EGFR and JNK1/2 that led to c-Jun activation. PMID: 27299855
  14. ths work provides transcriptional catalog for the developing inner circular smooth muscle and suggests that cJun regulates gene expression in the ICM downstream of Hh signaling. PMID: 26930384
  15. Jun is required to upregulate a programme of genes associated with cell adhesion as Embryonic stem cells exit the pluripotent ground state. PMID: 26850660
  16. ANXA11 level regulates lymph node metastases and 5-FU resistance of Hca-P murine hepatocarcinoma cells via c-Jun pathway PMID: 26908448
  17. Taken together, these findings indicate that LT reduces c-Jun protein levels via two distinct mechanisms, thereby inhibiting critical cell functions, including cellular proliferation. PMID: 28893904
  18. Ca(2+) signaling pathway increases Nr4a1 expression in MA-10 Leydig cells, at least in part, by enhancing the recruitment of coactivator most likely through the MEF2, AP1, and CREB transcription factors thus demonstrating an important interplay between the Ca(2+) and cAMP pathways in regulating Nr4a1 expression. PMID: 26647388
  19. we performed cotransfections of AP-1 expression plasmids with different mouse Gja1 promoter/luciferase reporter constructs within TM3 Leydig and TM4 Sertoli cells.We showed that a functional cooperation between cJun and cFos activates Gja1 expression and requires an AP-1 DNA regulatory element located between -132 and -26 bp PMID: 28903063
  20. AP1 factors are important regulators of adult taste cell renewal and their downregulation negatively impacts taste maintenance. PMID: 27787515
  21. these data indicate that MEF2 and AP-1 confer antagonistic regulation of Hspb7 gene expression in skeletal muscle, with implications for autophagy and muscle atrophy. PMID: 27632998
  22. results suggest that fibroblasts, c-Jun, and IGF-1 play key roles in mediating stromal-epithelial interactions that are required for the therapeutic effects of finasteride in benign prostate epithelial cells PMID: 28196103
  23. These findings highlight a key role of the TLR4-NOS1-AP1 signaling axis in regulating macrophage polarization PMID: 28013342
  24. Data suggest that Sf1 and c-jun interact and cooperate to activate the Fdx1 promoter in MA-10 (tumorigenic cell line) and TM3 (non-tumorigenic cell line) Leydig cells; such activation requires different regulatory elements located between -124 and -306 bp of Fdx1 promoter and involves recruitment of Sf1 to this region. (Sf1 = splicing factor 1; c-jun = proto-oncogene c-jun; Fdx1 = ferredoxin 1) PMID: 28274746
  25. NF-kappaB and c-Jun coregulate lipopolysaccharide-induced Fra-1 transcription. PMID: 27286066
  26. BATF/JUN-B and BATF/C-JUN complexes play important roles in OA cartilage destruction through regulating anabolic and catabolic gene expression in chondrocytes. PMID: 27147707
  27. this study shows that c-Jun regulates the activation state of macrophages and promotes arthritis via differentially regulating cyclooxygenase-2 and arginase-1 levels PMID: 28298526
  28. PLIO treatment inhibited nuclear factorkappaB (NFkappaB) nuclear translocation in B16F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun Nterminal kinases and AKT PMID: 27666322
  29. Anxa5 mediates the in vitro malignant behaviours of murine hepatocarcinoma Hca-F cells via ERK2/c-Jun/p-c-Jun(Ser73) and ERK2/E-cadherin pathways. PMID: 27697636
  30. These findings indicate that c-Jun has important functions during HBV-associated tumorigenesis by promoting hepatocyte proliferation as well as progression of dysplasia PMID: 26470729
  31. The TLR4 pathway may play an important role in regulating AP-1 activation PMID: 26803521
  32. These observations suggest that the antiinflammatory properties of crebanine may stem from the inhibition of proinflammatory mediators via suppression of the NF-kappaB, AP-1, MAPKs, and Akt signaling pathways. PMID: 26499331
  33. In a model of heavy metal poisoning, copper contamination of drinking water (as can occur with corrosion in aging plumbing systems) up-regulates binding of transcription factor AP-1 in neurons of brain, even in low concentrations. PMID: 23719850
  34. Transplant-induced reactivation of murine cytomegalovirus immediate early gene expression is associated with recruitment of NF-kappaB and AP-1 to the major immediate early promoter. PMID: 26795571
  35. ERK contributes to the morphine-induced hyperalgesia by regulating the activation of c-JUN PMID: 26165762
  36. These results indicate that both NF-kappaB and c-Jun/AP-1 are involved in regulating anti-beta2GPI-induced expression of prothrombotic and proinflammatory molecules in vivo. PMID: 26829121
  37. Tumorigenesis by Meis1 overexpression is accompanied by a change of DNA target-sequence specificity which allows binding to the AP-1 element. PMID: 26259236
  38. BMP7 and FGF9 coordinately regulate AP-1 transcription to promote G1-S cell cycle progression and nephron progenitor cells proliferation. PMID: 26634297
  39. Our findings conclude that pharmacological actions of CoQ0 are mediated via inhibition of NFkappaB/AP-1 activation and induction of Nrf2/ARE-signaling. PMID: 26548719
  40. Taken together, our results suggest that LPS induces PDK4 expression and alters glucose metabolism via the JNK pathway. PMID: 26740179
  41. In the heterologous mouse model loss of c-Jun expression led to ablation of dissemination of Theileria annulata-infected and transformed macrophages. PMID: 25375322
  42. Our results demonstrate that c-Jun can suppress adipocyte differentiation through the down-regulation of KLF15 at the transcriptional level. PMID: 26692489
  43. the regulation of synaptic-vesicle (SV) recycling via early endosomes by the interdependent regulation of AP-2-mediated endocytosis and AP-1/sigma1B-mediated SV reformation, is reported. PMID: 25128028
  44. A reciprocal antagonism between the MITF and c-Jun interconnects inflammation-induced dedifferentiation with pro-inflammatory cytokine responsiveness of melanoma cells favouring myeloid cell recruitment. PMID: 26530832
  45. Data indicate that Sp1 and AP-1-related factors are involved in the regulation of MFG-E8 gene transcription by targeting their binding sites in the 5'-flanking region under physiological and inflammatory states. PMID: 25711369
  46. Data show that p38 MAP Kinase (p38MAPK)-shRNA transfection and the p38MAPK inhibitor SB203580 significantly inhibited activator protein-1 (AP-1) activity. PMID: 25966080
  47. AS-IV effectively inhibits LPS-induced acute inflammatory responses by modulating NF-kappaB and AP-1 signaling pathways PMID: 25960613
  48. suggest that TLR-mediated Sesn2 induction is dependent on AP-1, Nrf2, and the inhibition of ubiquitin-mediated degradation of Sesn2 and might protect cells against endotoxin toxicity PMID: 25637945
  49. Dioxin exposure markedly inhibited c-Jun phosphorylation in Map3k1-deficient embryonic eyelid epithelium, suggesting that dioxin-induced AHR pathways can synergize with gene mutations to inhibit MAP3K1 signaling. PMID: 26109068
  50. The NF-kappaB-to-AP-1 switch is a protective mechanism to ensure timely transition from endothelial barrier injury to repair, accelerating barrier restoration following MOI. PMID: 24986487

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Subcellular Location Nucleus
Protein Families BZIP family, Jun subfamily
Database Links

KEGG: mmu:16476

STRING: 10090.ENSMUSP00000054270

UniGene: Mm.275071

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