MAP2K1, mitogen-activated protein kinase kinase 1, alias MEK1, MAPKK1, is a bispecific protein kinase, a member of the MAP kinase kinase kinase family, which is encoded by its own genes in human cells.MAP2K1 plays a key role in extracellular signaling, especially in its function in the Ras-Raf-MEK-ERK signaling pathway, which is involved in the regulation of cell proliferation, differentiation, and apoptosis. MAP2K1 plays a key role in extracellular signaling, especially in the Ras-Raf-MEK-ERK signaling pathway, which is involved in the regulation of cell proliferation, differentiation, apoptosis, etc. MAP2K1 acts by receiving the phosphorylation signals from the upstream RAF kinase, which phosphorylates and activates the downstream ERK1/2, and then transmits the signals to the nucleus and affects the expression of genes.
The biological significance of MAP2K1 is very broad, and it is involved in the regulation of a variety of cellular processes, such as cell proliferation, differentiation, transcriptional regulation, and development.The activity of MAP2K1 is critical for cellular response to a variety of external and internal signals. In addition, aberrant function of MAP2K1 has been associated with a variety of diseases, especially closely related to tumor development. For example, mutations in genes such as Raf and MEK1 may lead to the sustained activation of the Ras-Raf-MEK-ERK signaling pathway, which is considered to be one of the important causes of the infinite proliferative properties of tumors. Therefore, MEK1/MEK2 is often used as a target for tumor therapy in ERK signaling pathway research to develop novel anti-tumor drugs, such as selumetinib, an oral MEK inhibitor.
MAP2K1 Recombinant Monoclonal Antibody (CSB-RA225579A0HU)
Validated Data
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate
All lanes: MAP2K1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 41 kDa
Observed band size: 44 kDa
IHC image of CSB-RA225579A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating MAP2K1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA225579A0HU in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA225579A0HU(2µg)+ Hela whole cell lysate(500µg)
Lane 3: Hela whole cell lysate (10µg)
The following MAP2K1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
MAP2K1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA439786 | Phospho-MAP2K1 (Ser221) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA297521 | Phospho-MAP2K1 (Ser217/Ser221) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA241335 | Phospho-MAP2K1 (Thr291) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA920819 | MAP2K1 (Ab-221) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA065412 | MAP2K1 (Ab-217/221) Antibody | Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA965933 | MAP2K1 (Ab-291) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA577205 | MAP2K1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA003217 | MAP2K1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA030296 | Phospho-MAP2K1 (T292) Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA030297 | Phospho-MAP2K1 (S298) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA030298 | Phospho-MAP2K1 (T386) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA030299 | Phospho-MAP2K1 (T286) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA030300 | MAP2K1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA080228 | MAP2K1 Antibody | Human,Mouse,Rat | WB, IHC |
CSB-PA173019 | MAP2K1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA925811 | MAP2K1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-PA439945 | MAP2K1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA570222 | MAP2K1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA146432 | MAP2K1 Antibody | Human,Mouse,Rat | WB,ELISA |
CSB-PA013409LA01HU | MAP2K1 Antibody | Human, Mouse | ELISA, WB, IHC |
CSB-PA013409LB01HU | MAP2K1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA013409LC01HU | MAP2K1 Antibody, FITC conjugated | Human | |
CSB-RA013409A292phHU | Phospho-MAP2K1 (T292) Recombinant Monoclonal Antibody | Human | ELISA, WB |
CSB-RA225579A0HU | MAP2K1 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IP |
CSB-RA957619A0HU | MAP2K1 Recombinant Monoclonal Antibody | Human, Rat | ELISA, WB, IHC, IP |
MAP2K1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP013409MO CSB-EP013409MO CSB-BP013409MO CSB-MP013409MO CSB-EP013409MO-B |
Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAP2K1 Proteins for Oryctolagus cuniculus (Rabbit)
Code | Product Name | Source |
---|---|---|
CSB-YP013409RB CSB-EP013409RB CSB-BP013409RB CSB-MP013409RB CSB-EP013409RB-B |
Recombinant Rabbit Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAP2K1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP013409RA CSB-EP013409RA CSB-BP013409RA CSB-MP013409RA CSB-EP013409RA-B |
Recombinant Rat Dual specificity mitogen-activated protein kinase kinase 1 (Map2k1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAP2K1 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Source |
---|---|---|
CSB-YP726940DXU CSB-EP726940DXU CSB-BP726940DXU CSB-MP726940DXU CSB-EP726940DXU-B |
Recombinant Cricetulus griseus Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAP2K1 Proteins for Serinus canaria (Island canary) (Fringilla canaria)
Code | Product Name | Source |
---|---|---|
CSB-YP849582SYL CSB-EP849582SYL CSB-BP849582SYL CSB-MP849582SYL CSB-EP849582SYL-B |
Recombinant Serinus canaria Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAP2K1 Proteins for Pan troglodytes (Chimpanzee)
Code | Product Name | Source |
---|---|---|
CSB-YP893171EQV CSB-EP893171EQV CSB-BP893171EQV CSB-MP893171EQV CSB-EP893171EQV-B |
Recombinant Pan troglodytes Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |