Code | CSB-RA225579A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
CUSABIO employed a human MAP2K1-derived peptide to immunize an animal, stimulating an immune response. Following immunization, B cells were isolated from the animal and fused with myeloma cells, resulting in the creation of hybridoma cells. From these cells, a single hybridoma clone producing the desired MAP2K1-specific antibody was carefully screened and selected. RNA was subsequently extracted from the chosen hybridoma clone, and the MAP2K1 antibody-encoding genes were specifically amplified using reverse transcription PCR techniques. These amplified genes were then successfully cloned into an expression vector, which was subsequently introduced into a host system for efficient expression. Through affinity chromatography, the resulting MAP2K1 recombinant monoclonal antibodies were purified from the cell culture supernatant. Rigorous validation, employing ELISA, WB, IHC, and IP techniques, confirmed the binding specificity and affinity of the recombinant monoclonal MAP2K1 antibody, which notably recognizes human MAP2K1 protein.
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