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Product Name:

Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

Product Type : ELISA Kit
Code : CSB-E11718h
Size : 96T,5×96T,10×96T
Uniprot NO. : P15692
Abbreviation : VEGFA
Protein Biological Process 1 : Cytokine
Alias : RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
Species : Human
Protein Biological Process 3 : Angiogenesis
Sample Types : serum, plasma, cell culture supernates, tissue homogenates, cell lysates and urine
Detect Range : 31.25 pg/ml-2000 pg/ml
Sensitivity : 25.297 pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
HGNC : 12680
RGD : 619991
MGI : 103178
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %98
Range %94-106
1:2Average %93
Range %86-97
1:4Average %87
Range %82-93
1:8Average %96
Range %91-105
Recovery :
The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8985-93
EDTA plasma (n=4)9290-96
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
20002.651 2.751 2.701 2.547
10001.729 1.829 1.779 1.625
5001.067 1.087 1.077 0.923
2500.579 0.589 0.584 0.430
1250.372 0.382 0.377 0.223
62.50.255 0.265 0.260 0.106
31.250.207 0.209 0.208 0.054
00.153 0.154 0.154  
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