Code | CSB-PA07889A0Rb |
Size | US$166 |
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The TP53 Antibody (Product code: CSB-PA07889A0Rb) is Non-conjugated. For TP53 Antibody with conjugates, please check the following table.
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:20-1:200 |
Applications : Immunoblotting
Sample type: Cell
Review: The protein levels of proliferation, EMT, and apoptosis transcription factors (p53, c-Myc, NF-κB, Zeb1, Zeb2, Snail, Twist1) in BPH rats\' prostate were determined using Immunoblotting.
By Anonymous
Applications : Western Blot (WB)
Sample type: Human fallopian tube epithelium cell line
Sample dilution: 1:2000
Review: It worked decently. I was positively surprised by the result given the high dilution of 1:2000 There was a slight additional band around 70kDa, otherwise fairly specific. Image: Green=P53, red=Beta-Actin FT282 cell line, 30ug input BOLT Bis-Tris Gel 4-12%, BioRad BOLT Transfer buffer with 10% Methanol, PVDF membrane Blocking: 1hr at RT, 5% BLOTTO milk powder in TBS-T anti P53 1:2000, 4C overnight secondary: anti Rabbit 680 IRDye 1:5000, 1hr RT Imaged with Licor scanner
By Anonymous