Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) Rac1 Polyclonal antibody

Uniprot No.

P40792

Target Names

Rac1

Alternative Names

Rac1 antibody; RacA antibody; CG2248 antibody; Ras-related protein Rac1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) Rac1 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

During various developmental processes, regulates changes in cell morphology in response to extracellular signals. During oogenesis, mediates signaling from the tyrosine kinase (RTK) chemoattractant receptors (Egfr and Pvr) to the guidance pathway that control the directional persistent collective migration of the border cell (BC) cluster through the nurse cells to the oocyte. Once activating by Pvr and Egfr, promotes the formation of forward-directed actin protrusions which stabilize the DE-cadherin (shg)-mediated adhesions. In turn, DE-mediated adhesion between the leader border cells and nurse cells further promotes Rac1 signaling creating a positive feedback loop that amplifies the output of RTK activity and leads to higher Rac activity at the front, thus promoting polarization of the border cell cluster and directionally persistent migration. Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.

Gene References into Functions

Behavior-evoked activation of Rac1-dependent forgetting has a converging function for autism-risk genes. PMID: 27335463
Rac1, a small GTPase, is specifically required in Eiger-mediated cell death. rac1 loss of function blocks Eiger-induced cell death, whereas Rac1 overexpression enhances Eiger-induced killing. PMID: 27054336
PERK/ATF4 activated the JNK pathway through Rac1 and Slpr activation in apoptotic cells. PMID: 25299777
The small GTPase RAC1 is a driver of intestinal stem cell proliferation through the production of reactive oxygen species. PMID: 23974108
Rac is asymmetrically activated in germline stem cells. PMID: 22802725
These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing. PMID: 22449966
Apical proteins including beta(H) are negatively regulated by Rac1 activation. PMID: 21111816
Mbc is crucial for the integrity of the F-actin foci and the FCM cytoskeleton, presumably via its activation of Rac1 in these cells. PMID: 21389053
Study reports that a small G protein Rac-dependent forgetting mechanism contributes to both passive memory decay and interference-induced forgetting in Drosophila. PMID: 20178749
Rac1, Rac2 and Mtl, have overlapping functions in the control of epithelial morphogenesis, myoblast fusion, and axon growth and guidance PMID: 11919634
axon growth, guidance and branching could be controlled by differential activation of Rac signalling pathways PMID: 11919635
Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila. PMID: 11973353
Rac1 is partially responsible for mediating the effect of Fmr1 on dendritic development. PMID: 14530299
Both Rac1 and Cdc42 activation induced large growth cones and long filopodia, but Cdc42 did so more efficiently than Rac1. Only Rac1 activation, however, induced thick actin bundles in the filopodia. PMID: 14750960
Rac plays multiple roles in epithelial sheet fusion, including dorsal closure. PMID: 15936337
Rac1 is involved in the regulation of myoblast proliferation and segregation during adult myogenesis. PMID: 16125691
Rac1 induces Bsk activity and stable actin formation for cellular immune activation, leading to sessile hemocyte release and an increase in the number of circulating hemocytes. PMID: 16621891
Reducing activity of the Egfr, Vav, or Rac1 from somatic support cells enhanced the germ cell enclosure defects of a conditional Spitz allele. PMID: 17629483

Hide All

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Rho family

Tissue Specificity

Detected in oocyte border cells (BC), with increased expression at the front of the BC cluster.

Database Links

KEGG: dme:Dmel_CG2248

STRING: 7227.FBpp0304252

UniGene: Dm.4299

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA331223XA01DLU
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

Rac1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background