Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays a key role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10, which suppresses inflammatory responses by inhibiting pro-inflammatory cytokine production and limiting antigen presentation. This immunosuppressive activity makes IL-10 essential for preventing excessive immune activation. It also maintains tissue integrity during inflammatory processes. Research into IL-10 levels provides valuable insights into immune dysfunction, autoimmune diseases, and inflammatory conditions across various species.
The Bovine Interleukin 10, IL-10 ELISA Kit (CSB-E12917B) is designed for quantitative measurement of IL-10 in Bos taurus samples. This competitive assay works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 1000 pg/mL with sensitivity of 2.5 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for bovine IL-10 quantification in immunological studies.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This bovine interleukin 10 ELISA kit has been used in research investigating immune responses and inflammatory processes in cattle. The kit allows quantitative measurement of IL-10 levels in various biological samples to determine anti-inflammatory cytokine profiles and immune system function.
• Immune response studies: Measurement of immunological parameters alongside other immune markers including immunoglobulins and pro-inflammatory cytokines in serum samples
• Antioxidant and inflammation research: Analysis of anti-inflammatory cytokine levels in conjunction with oxidative stress markers to understand the relationship between antioxidant capacity and immune function
• Pro-inflammatory response analysis: Measurement of anti-inflammatory cytokines in plasma samples as part of comprehensive cytokine profiling studies
• Tissue-based cytokine analysis: Quantification of IL-10 levels in lymph node tissue samples for localized immune response studies
