Bovine Interleukin 10,IL-10 ELISA Kit

Bovine Interleukin 10,IL-10 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

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Customer Reviews and Q&A

Target Background

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Bovine Interleukin 10,IL-10 ELISA Kit

Citations (15) Protocol

Code	CSB-E12917B
Size	96T,5×96T,10×96T
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Product Details
Citations (15)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 10

Alternative Names

IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit

Abbreviation

IL10

Uniprot No.

P43480

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

5 pg/mL-1000 pg/mL

Sensitivity

2.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-98
1:2	Average %	95
	Range %	91-98
1:4	Average %	96
	Range %	87-102
1:8	Average %	97
	Range %	91-104

Recovery

The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	95-100
EDTA plasma (n=4)	96	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.098	0.095	0.097
250	0.301	0.285	0.293
80	0.487	0.458	0.473
20	0.972	0.975	0.974
5	1.364	1.316	1.340
0	2.128	2.211	2.170

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Bovine IL10 ELISA Kit was designed for the quantitative measurement of Bovine IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.

Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea ZL Fu,Journal of dairy science,2024
Antimicrobial Resistance of Staphylococcus aureus and Coagulase‑negative Staphylococci from Bovine Mastitis Milk with Detection of Interleukins in Milk and Serum of Infected Cows A Shehab,Journal of Advanced Veterinary Research,2023
The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
Tropical study on Clinical and Host Cell Responses of Acute Phase Proteins, Pro-Inflammatory Cytokines and Antibodies’ in Lactating Dairy Cows Vaccinated and Challenged with S. aureus IU Hambali,ResearchGate,2022
Phyllanthus emblica (Amla) Fruit Powder as a Supplement to Improve Preweaning Dairy Calves’ Health: Effect on Antioxidant Capacity, Immune Response, and Gut Bacterial Diversity M Nguse,Biology,2022
Effect of non-encapsulated and encapsulated active dried yeast on blood cell count, blood metabolites and immune response of finishing beef heifers P Jiao,Canadian Journal of Animal Science ja,2021
In-vitro analysis of Interleukin-10 expression in cell cultures of Crossbred cattle, Tharparkar cattle and Murrah buffalo in response to mastitis causing antigens derived from Staphylococcus aureus and Escherichia coli Sourabh Sulabh, et al,Biological Rhythm Research,2019
Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows Hambali IU, et al,Tropical animal health and production,2018
Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows Hambali IU, et al,Tropical animal health and production,2018
Effect of in vitro zinc supplementation on HSPs expression and Interleukin 10 production in heat treated peripheral blood mononuclear cells of transition Sahiwal and Karan Fries cows Sheikh AA.et al,J Therm Biol.,2016
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016
Differential IFN-Gamma (IFN-r), Interleukin 10 (IL-10) and Cardiac Troponin I (cTnI) Responses in Natural Bovine Trypanosomosis in Nigeria Michael I. Takeet1.et al,Open Journal of Veterinary Medicine,2016
Molecular and pathological studies on natural cases of bovine theileriosis Gupta A.et al,Journal of Parasitic Diseases,2016
Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,2015
Increased blood-circulating interferon-r, interleukin-17, and osteopontin levels in bovine paratuberculosis Dudemaine PL et al,J Dairy Sci,2014

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Function
(From Uniprot)

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.

Gene References into Functions

Studied expression of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows. Found IL-10 expression to be significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. PMID: 28267404
This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10. PMID: 25286662
IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes. PMID: 22052007
Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation. PMID: 21464967
Levels in milk are altered in the presence of bacteria. PMID: 15372997
Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses. PMID: 16111147

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Subcellular Location

Secreted.

Protein Families

IL-10 family

Database Links

KEGG: bta:281246

STRING: 9913.ENSBTAP00000008793

UniGene: Bt.4723

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Email: [email protected]
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Related pathways

Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.