Bovine Interleukin 10,IL-10 ELISA Kit

Code CSB-E12917B
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P43480
Lead Time 3-5 working days
Abbreviation IL10
Target Name interleukin 10
Alias CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 pg/mL-1000 pg/mL
Sensitivity 2.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
HGNC 5962
RGD 2886
MGI 96537
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 89-98  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 96  
Range % 87-102  
1:8 Average % 97  
Range % 91-104  
Recovery
The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 95-100  
EDTA plasma (n=4) 96 92-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.098 0.095 0.097    
250 0.301 0.285 0.293    
80 0.487 0.458 0.473    
20 0.972 0.975 0.974    
5 1.364 1.316 1.340    
0 2.128 2.211 2.170    
References
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Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: bta:281246

STRING: 9913.ENSBTAP00000008793

UniGene: Bt.4723

Pathway Jak-STAT signaling pathway
T cell receptor signaling pathway
FoxO signaling pathway

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