Bovine Interleukin 8,IL-8 ELISA Kit

Bovine Interleukin 8,IL-8 ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

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ELISA Kit
Bovine Interleukin 8,IL-8 ELISA Kit

Citations (10) Protocol

Code	CSB-E13052B
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Citations (10)
Related Products
Customer Reviews and Q&A
Target Background

Target Name

interleukin 8

Alternative Names

CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit

Abbreviation

CXCL8

Uniprot No.

P79255

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

50 pg/mL-2000 pg/mL

Sensitivity

50 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	94
	Range %	86-100
1:4	Average %	88
	Range %	80-94
1:8	Average %	92
	Range %	86-100

Recovery

The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-106
EDTA plasma (n=4)	98	94-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
0	2.655	2.514	2.585
50	1.279	1.232	1.256
150	0.611	0.638	0.625
460	0.342	0.363	0.353
1000	0.218	0.224	0.221
2000	0.125	0.131	0.128

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Bovine IL8 ELISA Kit was designed for the quantitative measurement of Bovine IL8 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 50 pg/mL-2000 pg/mL and the sensitivity is 50 pg/mL.

Screening of Some Changes in Hematological, Serum Biochemical, Inflammatory and Oxidative Parameters Associated with Pathogenesis of Retained Placenta in Holstein Dairy Heifers D El-Karim, Journal of Applied Veterinary Sciences,2023
The effect of staphylococcal mastitis including resistant strains on serum procalcitonin, neopterin, acute phase response and stress biomarkers in Holstein dairy cows W El-Deeb,PeerJ,2021
Penta-acetyl Geniposide Suppresses Migration, Invasion, and Inflammation of TNF-α-Stimulated Rheumatoid Arthritis Fibroblast-Like Synoviocytes Involving Wnt/β-Catenin Signaling Pathway L Cai,Inflammation,2021
Modelling early events in Mycobacterium bovis infection using a co-culture model of the bovine alveolus DF Lee,Scientific reports,2020
Subclinical endometritis and postpartum ovarian resumption in respect to TNF-α, IL-8 and CRP in Egyptian buffaloes DH Elsayed,animal reproduction,2020
Caffeic Acid Prevented LPS-Induced Injury of Primary Bovine Mammary Epithelial Cells through Inhibiting NF-κB and MAPK Activation Mingjiang Liu,Mediators of Inflammation,2019
Supplementation with fat-coated rumen-protected glucose during the transition period enhances milk production and influences blood biochemical parameters of liver function and inflammation in dairy cows X.P.Li,Animal Feed Science and Technology,2019
Interferon tau stimulated gene expression and proinflammatory cytokine profile relative to insemination in dairy cows Aasif Ahmad Sheikh.et al,Biological Rhythm Research ,2018
Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,2017
A small scale study on the effects of oral administration of the ß-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves Hirofumi Uchiyama et al,BMC Research Notes,2012

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Function
(From Uniprot)

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.

Gene References into Functions

Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-kappaB activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1. PMID: 29101849
STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342
As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease. PMID: 27813832
Blood levels of cortisol, immunoglobulin G, and IL-8 in relationship to genetics and the incidence of bovine respiratory complex disease are reported. PMID: 27482664
Genetic variation at the IL8 locus explains a proportion of the inter-breed and inter-individual variation in immunity between neonatal calves which is likely to influence their resistance to infection. PMID: 27863538
In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
Studied association of IL8 -105G/A with mastitis somatic cell score in Chinese Holstein dairy cows. Results suggest genotype GG may be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein dairy cows. PMID: 25380467
Data implicate a complex cascade of events during luteolysis, involving Il8 signaling, neutrophil recruitment, and immune cell action within the corpus luteum. PMID: 24686456
Cadmium exposure induced pulmonary inflammation and increased lung interleukin 8 secretion. PMID: 22094458
Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
study demonstrates that a small experimental rodent model can be utilized for non-invasively monitoring, through a reporter gene system, the activation of an IL-8 promoter region derived from a larger size animal (bovine) PMID: 22761878
Finding of genetic variation in the IL8 promoter that differentially regulates its expression has significant functional implications for IL8 expression in vitro and which may impact on susceptibility to infectious disease and inflammation. PMID: 22244152
Polymorphonuclear neutrophils migrate into the early corpus luteum(CL) partially due to its major chemoattractant IL-8 produced at high levels in the CL. PMID: 21651784
IL8 genotype significantly correlated with mastitis resistance and the locus could be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein. PMID: 21113675
IL8 promoter serial deletion analysis revealed a specific region responsive to ORF50/Rta; may represent an endometrial defense mechanism against viral infection PMID: 20720165
IL8 gene genetic diversity has a great genetic effect on milk traits and mastitis resistance PMID: 21513151
The role of NF-kappaB and C/EBP factors in regulating basal and pathogen-induced expression of both genes from cattle, is investigated. PMID: 21255844
Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
Levels in milk are altered in the presence of bacteria PMID: 15372997
C-terminal of both bovine IL-8R showed 100% identity, whereas they differ in most other species, suggesting that the two bovine IL-8R initiate similar signal transduction. PMID: 17727952
the pathogen causing subclinical mastitis impairs NF-kappaB activation in MEC thereby severely weakening the immune response,induction of IL-8 and TNFalpha, in the udder PMID: 17936907

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

KEGG: bta:280828

STRING: 9913.ENSBTAP00000026275

UniGene: Bt.49740

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Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway Cellular senescence IL-17 signaling pathway NOD-like receptor signaling pathway Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Oxidative Phosphorylation Phospholipase D signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.