Bovine Interleukin 8,IL-8 ELISA Kit

Instructions
Code CSB-E13052B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 8
Alternative Names CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic
Abbreviation IL8
Uniprot No. P79255
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 50 pg/mL-2000 pg/mL
Sensitivity 50 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-95  
1:2 Average % 94  
Range % 86-100  
1:4 Average % 88  
Range % 80-94  
1:8 Average % 92  
Range % 86-100  
Recovery
The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 95-106  
EDTA plasma (n=4) 98 94-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
0 2.655 2.514 2.585    
50 1.279 1.232 1.256    
150 0.611 0.638 0.625    
460 0.342 0.363 0.353    
1000 0.218 0.224 0.221    
2000 0.125 0.131 0.128    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (By similarity).
Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: bta:280828

STRING: 9913.ENSBTAP00000026275

UniGene: Bt.49740

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