Bovine Interleukin 8,IL-8 ELISA Kit

Instructions
Code CSB-E13052B
Size 96T,5×96T,10×96T
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Product Details

Target Name interleukin 8
Alternative Names CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
Abbreviation IL8
Uniprot No. P79255
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 50 pg/mL-2000 pg/mL
Sensitivity 50 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 82-95  
1:2 Average % 94  
Range % 86-100  
1:4 Average % 88  
Range % 80-94  
1:8 Average % 92  
Range % 86-100  
Recovery
The recovery of bovine IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 100 95-106  
EDTA plasma (n=4) 98 94-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
0 2.655 2.514 2.585    
50 1.279 1.232 1.256    
150 0.611 0.638 0.625    
460 0.342 0.363 0.353    
1000 0.218 0.224 0.221    
2000 0.125 0.131 0.128    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus (By similarity).
Gene References into Functions
  1. Molecular characterization of the bovine IER3 gene: Down-regulation of IL-8 by blocking NF-kappaB activity mediated by IER3 overexpression in MDBK cells infected with bovine viral diarrhea virus-1. PMID: 29101849
  2. STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342
  3. As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease. PMID: 27813832
  4. Blood levels of cortisol, immunoglobulin G, and IL-8 in relationship to genetics and the incidence of bovine respiratory complex disease are reported. PMID: 27482664
  5. Genetic variation at the IL8 locus explains a proportion of the inter-breed and inter-individual variation in immunity between neonatal calves which is likely to influence their resistance to infection. PMID: 27863538
  6. In response to C. abortus infection, both ovine and bovine turbinate cells produce CXCL8 mRNA and protein late in the bacterial developmental cycle. PMID: 26342452
  7. Studied association of IL8 -105G/A with mastitis somatic cell score in Chinese Holstein dairy cows. Results suggest genotype GG may be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein dairy cows. PMID: 25380467
  8. Data implicate a complex cascade of events during luteolysis, involving Il8 signaling, neutrophil recruitment, and immune cell action within the corpus luteum. PMID: 24686456
  9. Cadmium exposure induced pulmonary inflammation and increased lung interleukin 8 secretion. PMID: 22094458
  10. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
  11. study demonstrates that a small experimental rodent model can be utilized for non-invasively monitoring, through a reporter gene system, the activation of an IL-8 promoter region derived from a larger size animal (bovine) PMID: 22761878
  12. Finding of genetic variation in the IL8 promoter that differentially regulates its expression has significant functional implications for IL8 expression in vitro and which may impact on susceptibility to infectious disease and inflammation. PMID: 22244152
  13. Polymorphonuclear neutrophils migrate into the early corpus luteum(CL) partially due to its major chemoattractant IL-8 produced at high levels in the CL. PMID: 21651784
  14. IL8 genotype significantly correlated with mastitis resistance and the locus could be a useful genetic marker for mastitis resistance selection and breeding in Chinese Holstein. PMID: 21113675
  15. IL8 promoter serial deletion analysis revealed a specific region responsive to ORF50/Rta; may represent an endometrial defense mechanism against viral infection PMID: 20720165
  16. IL8 gene genetic diversity has a great genetic effect on milk traits and mastitis resistance PMID: 21513151
  17. The role of NF-kappaB and C/EBP factors in regulating basal and pathogen-induced expression of both genes from cattle, is investigated. PMID: 21255844
  18. Data describe the expression of IL-8, IL-1beta and their respective receptors, CXCR1 and IL-1R1 in bovine theca cells, and suggest that VEGF is associated with the IL system in theca cells in ovary. PMID: 20156197
  19. Levels in milk are altered in the presence of bacteria PMID: 15372997
  20. C-terminal of both bovine IL-8R showed 100% identity, whereas they differ in most other species, suggesting that the two bovine IL-8R initiate similar signal transduction. PMID: 17727952
  21. the pathogen causing subclinical mastitis impairs NF-kappaB activation in MEC thereby severely weakening the immune response,induction of IL-8 and TNFalpha, in the udder PMID: 17936907

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: bta:280828

STRING: 9913.ENSBTAP00000026275

UniGene: Bt.49740

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