Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

Instructions
Code CSB-E04568h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name colony stimulating factor 2 (granulocyte-macrophage)
Alternative Names GMCSF, MGC131935, MGC138897, colony stimulating factor 2|granulocyte-macrophage colony stimulating factor|molgramostin|sargramostim
Abbreviation CSF2
Uniprot No. P04141
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 82-99
1:2 Average % 95
Range % 88-102
1:4 Average % 95
Range % 86-99
1:8 Average % 95
Range % 90-105
Recovery
The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-100
EDTA plasma (n=4) 96 90-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.987 2.913 2.950 2.792
500 2.417 2.429 2.423 2.265
250 1.674 1.686 1.680 1.522
125 0.954 0.963 0.959 0.801
62.5 0.576 0.562 0.569 0.411
31.2 0.386 0.394 0.390 0.232
15.6 0.261 0.268 0.265 0.107
0 0.156 0.159 0.158  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subcellular Location Secreted
Protein Families GM-CSF family
Database Links

HGNC: 2434

OMIM: 138960

KEGG: hsa:1437

STRING: 9606.ENSP00000296871

UniGene: Hs.1349

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