Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

Code CSB-E04568h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P04141
Lead Time 3-5 working days
Abbreviation CSF2
Target Name colony stimulating factor 2 (granulocyte-macrophage)
Alias GMCSF, MGC131935, MGC138897, colony stimulating factor 2|granulocyte-macrophage colony stimulating factor|molgramostin|sargramostim
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
HGNC 2436
RGD 620189
MGI 1339759
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 82-99
1:2 Average % 95
Range % 88-102
1:4 Average % 95
Range % 86-99
1:8 Average % 95
Range % 90-105
Recovery
The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 86-100
EDTA plasma (n=4) 96 90-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.987 2.913 2.950 2.792
500 2.417 2.429 2.423 2.265
250 1.674 1.686 1.680 1.522
125 0.954 0.963 0.959 0.801
62.5 0.576 0.562 0.569 0.411
31.2 0.386 0.394 0.390 0.232
15.6 0.261 0.268 0.265 0.107
0 0.156 0.159 0.158  
References
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Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subcellular Location Secreted
Protein Families GM-CSF family
Database Links

HGNC: 2434

OMIM: 138960

KEGG: hsa:1437

STRING: 9606.ENSP00000296871

UniGene: Hs.1349

Pathway Jak-STAT signaling pathway
TNF signaling pathway
Fc epsilon RI signaling pathway
Hematopoietic cell lineage
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
T cell receptor signaling pathway

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