Recombinant Mouse Interleukin-13 (Il13) is expressed in a mammalian cell system and features a C-terminal 6xHis-tag for ease of purification. The protein represents a partial sequence spanning amino acids 26-131 and shows purity greater than 95% as verified by SDS-PAGE analysis. Biological activity appears robust, with an ED50 of 2-13 ng/ml in cell proliferation assays using TF-1 human erythroleukemic cells. Endotoxin levels remain below 1.0 EU/µg, as determined by the LAL method.
Interleukin-13 is a cytokine that plays a role in immune regulation and inflammation. T-helper type 2 cells primarily produce this protein, and it influences various cell types. This likely contributes to the pathophysiology of allergic responses and asthma. The protein serves as an important component for studying immune responses and understanding regulatory mechanisms in inflammatory processes.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Cell Proliferation and Viability Assays
This recombinant mouse IL-13 protein can stimulate cell proliferation in responsive cell lines, as shown by its validated activity on TF-1 human erythroleukemic cells with an ED50 of 2-13 ng/ml. Researchers may use this protein to investigate IL-13-mediated cellular responses in various mouse and cross-reactive human cell types. The high purity (>95%) and low endotoxin levels (<1.0 EU/μg) appear suitable for sensitive cell culture experiments where contamination could confound results. The C-terminal His-tag allows for straightforward detection and quantification in experimental setups.
2. Cytokine Signaling Pathway Studies
The biologically active mouse IL-13 protein serves as a useful tool for investigating IL-13 receptor binding and downstream signaling cascades in vitro. This protein enables researchers to study STAT6 phosphorylation, JAK activation, and other IL-13-mediated signaling events in mouse cell lines or primary cells. The mammalian expression system likely ensures proper protein folding and post-translational modifications that are crucial for authentic receptor interactions. The defined activity range provides a reasonable starting point for dose-response experiments in signaling studies.
3. Antibody Development and Validation
This recombinant mouse IL-13 protein can serve as an antigen for developing and validating anti-IL-13 antibodies in research applications. The C-terminal His-tag enables straightforward immobilization on nickel-based surfaces for ELISA development, antibody screening, and binding affinity measurements. High purity suggests minimal cross-reactivity with contaminating proteins during antibody characterization. Researchers may use this protein as a positive control in immunoassays and for antibody specificity testing in mouse-based research models.
4. Protein-Protein Interaction Studies
The His-tagged mouse IL-13 protein appears well-suited for pull-down assays and other protein interaction studies to identify IL-13 binding partners or receptor components. The tag enables protein immobilization on nickel-coated beads or columns for affinity purification experiments. Researchers can investigate IL-13 interactions with its receptors (IL-13Rα1, IL-13Rα2, IL-4Rα) or other potential binding proteins in cell lysates or purified protein systems. Low endotoxin content suggests that observed interactions are specific to IL-13 rather than contamination-induced artifacts.
5. Comparative Species and Cross-Reactivity Studies
This mouse IL-13 protein enables researchers to conduct comparative studies examining species-specific responses and cross-reactivity patterns between mouse and human IL-13 systems. The demonstrated activity on human TF-1 cells suggests potential cross-species functionality that can be systematically investigated across different cell types. Researchers may compare the potency and specificity of mouse IL-13 versus human IL-13 in various experimental systems. Such studies appear valuable for translational research where mouse models are used to study human IL-13-related pathways.
