CASP3 Antibody

Datasheet
Code CSB-PA240326
Product Type Polyclonal Antibody
Former Code(s) CSB-PA021967
Size US$297
Uniprot No. P42574
Image
  • Western blot analysis of extracts from 293, Hela and MDA cells using Caspase 3 Antibody.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.
Raised in Rabbit
Species Reactivity Human
Specificity The antibody detects endogenous level of total Caspase 3 protein.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Gervais, F. G et al.Cell 97: 395-406, 1999.
Nicholson, D. W. et al. (1995) Nature 376, 37-43.

Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias Apopain
Immunogen Species Homo sapiens (Human)
Target Names CASP3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

Pathway MAPK signaling pathway
p53 signaling pathway
TNF signaling pathway
Apoptosis
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
AGE-RAGE signaling pathway in diabetic complications
ER Stress pathway

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